1. APOBEC3C S188I Polymorphism Enhances Context-Specific Editing of Hepatitis B Virus Genome
- Author
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Pierre Khalfi, Rodolphe Suspène, Vincent Caval, Valérie Thiers, Guillaume Beauclair, Agnès Marchio, Claudine Bekondi, Marie Amougou Atsama, Serge Magloire Camengo-Police, Dominique Noah Noah, Richard Njouom, Hervé Blanc, Thomas Vallet, Marco Vignuzzi, Pascal Pineau, Jean Pierre Vartanian, Virologie (CNRS-UMR3569), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Département de Virologie - Department of Virology, Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP), Organisation Nucléaire et Oncogenèse / Nuclear Organization and Oncogenesis, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Institut Pasteur de Bangui, Réseau International des Instituts Pasteur (RIIP), Centre Pasteur du Cameroun, Hôpital Central de Yaoundé [Yaoundé], Hôpital de l'Amitié [Bangui], Populations virales et Pathogenèse - Viral Populations and Pathogenesis, This work was supported by the Institut Pasteur (grants to R. S., V. C., V. T., G. B., H. B., T. V., M. V., and J. P. V.), Institut Pasteur International Network (Action Concertée Inter-Pasteurienne grant number 17-2010 and Grant Dedonder to C. B., M. A. A., S. M. C. P., D. N. N., and R. N.), and and Ligue Contre le Cancer (Equipe Labélisée d’Anne Dejean to A. M. and P. P.). P. K. was supported by the Ligue Contre le Cancer.
- Subjects
Hepatitis B virus ,editing ,nucleotide context ,Genome, Viral ,Hepatitis B ,Polymorphism, Single Nucleotide ,patients ,polymorphism ,Infectious Diseases ,Cytidine Deaminase ,Africa ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,Humans ,APOBEC3C ,Immunology and Allergy - Abstract
Single-nucleotide polymorphism in APOBEC3C (resulting in a serine to isoleucine in position 188) is present in approximately 10% of African populations and greatly enhances restriction against human immunodeficiency virus-1 and simian immunodeficiency virus by improving dimerization and DNA processivity of the enzyme. In this study, we demonstrated in culture and in infected patients that hepatitis B virus (HBV) could be edited by APOBEC3CS188I. Using next-generation sequencing, we demonstrated that APOBEC3CS188I led to enhanced editing activity in 5ʹTpCpA→5ʹTpTpA context. This constitutes a new hallmark of this enzyme, which could be used to determine its impact on HBV or nuclear DNA.
- Published
- 2022
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