1. Genotype of 88 Toxoplasma gondii isolates associated with toxoplasmosis in immunocompromised patients and correlation with clinical findings
- Author
-
Thanh Hai Duong, C. Kauffmann-Lacroix, Sophie Pujol, Meja Rabodonirina, Luc Paris, Françoise Gay-Andrieu, Daniel Ajzenberg, Pierre Flori, B. Cuisenier, Francine Pratlong, Bernard Carme, Marie-Hélène Bessières, Hélène Yera, Jean Menotti, Dominique Aubert, Marie-Laure Dardé, Pierre Marty, N. Desbois, Marie-Elisabeth Bougnoux, Philippe Thulliez, Anne Totet, Denis Filisetti, Isabelle Villena, Dorothée Quinio, Laurence Delhaes, Hervé Pelloux, Florence Robert-Gangneux, Gilles Nevez, Jacqueline Franck, Chantal Duhamel, Frédéric Dalle, Henri Bonnabau, Université de Limoges (UNILIM), Neuroépidémiologie Tropicale et Comparée (NETEC), Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503)-Institut d'Epidémiologie Neurologique et de Neurologie Tropicale-Université de Limoges (UNILIM), Centre National de Référence (CNR) Toxoplasmose/Toxoplasma Biological Resource Center (BRC) (CNR Toxoplasmose-Toxoplasma BRC), CHU Limoges, Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (LUBEM), Université de Brest (UBO), and Service de l'Information Médicale et de l'Évaluation [CHU Limoges] (SIME)
- Subjects
medicine.medical_specialty ,Genotype ,Lung Diseases, Parasitic ,030231 tropical medicine ,Population ,Acquired Toxoplasmosis ,HIV Infections ,03 medical and health sciences ,Immunocompromised Host ,0302 clinical medicine ,Immunopathology ,Epidemiology ,parasitic diseases ,medicine ,Immunology and Allergy ,Animals ,Humans ,education ,Genotyping ,030304 developmental biology ,0303 health sciences ,education.field_of_study ,biology ,Toxoplasma gondii ,biology.organism_classification ,medicine.disease ,Virology ,Toxoplasmosis ,3. Good health ,Infectious Diseases ,Immunology ,Encephalitis ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,France ,Toxoplasma - Abstract
International audience; We report the genotyping analysis of Toxoplasma gondii isolates in samples collected from 88 immunocompromised patients, along with clinical and epidemiological data. Most of these samples were collected in France during the current decade by the Toxoplasma Biological Resource Center. Lack of specific anti-Toxoplasma treatment, pulmonary toxoplasmosis, and involvement of multiple organs were the 3 main risk factors associated with death for this patient group. Genotyping results with 6 microsatellite markers showed that type II isolates were predominant among patients who acquired toxoplasmic infection in Europe. Non-type II isolates included 13 different genotypes and were mainly collected from patients who acquired toxoplasmosis outside Europe. Type III was the second most common genotype recovered from patients, whereas type I was rare in our population. Three nonarchetypal genotypes were repeatedly recovered from different patients who acquired the infection in sub-Saharan Africa (genotypes Africa 1 and Africa 2) and in the French West Indies (genotype Caribbean 1). The distribution of genotypes (type II vs. non-type II) was not significantly different when patients were stratified by underlying cause of immunosuppression, site of infection, or outcome. We conclude that in immunocompromised patients, host factors are much more involved than parasite factors in patients' resistance or susceptibility to toxoplasmosis.
- Published
- 2009
- Full Text
- View/download PDF