1. ENHANCED MALIGNANT PROGRESSION OF NASOPHARYNGEAL CARCINOMA CELLS MEDIATED BY THE EXPRESSION OF EPSTEIN-BARR NUCLEAR ANTIGEN 1IN VIVO
- Author
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Chung Faye Chao, Ching Liang Meng, Fur Jiang Leu, Kuo Chieh Ho, Wei Hwa Lee, Lai Fa Sheu, and Ann Chen
- Subjects
Severe combined immunodeficiency ,biology ,biology.organism_classification ,medicine.disease ,medicine.disease_cause ,Epstein–Barr virus ,Pathology and Forensic Medicine ,Transplantation ,stomatognathic diseases ,Nasopharyngeal carcinoma ,Antigen ,Tumor progression ,hemic and lymphatic diseases ,Immunology ,medicine ,Carcinoma ,Cancer research ,Gammaherpesvirinae - Abstract
Nasopharyngeal carcinoma (NPC) is consistently associated with Epstein-Barr virus (EBV) and mostly classified as poorly differentiated squamous cell carcinoma or undifferentiated carcinoma with early metastasis and a rapidly progressive clinical course. The EBV-encoded latent proteins, Epstein-Barr nuclear antigen 1 (EBNA 1) and latent membrane proteins (LMPs), may be expressed in NPC, but their biological effects are poorly understood. EBNA 1 may predispose B lymphocytes to lymphomagenesis in transgenic mice, but its biological effects in NPC are still unknown. This study investigated the biological effects of EBNA 1 by expressing it in an EBV-negative NPC cell line (HONE-1), which was then inoculated into both nude and severe combined immunodeficiency mice. The EBNA 1 caused HONE-1 cells to grow in a less differentiated pattern and to progress more rapidly, as well as increasing their tumourigenicity and metastatic capability. These data suggest that EBNA 1 may play a critical role in the progressive evolution of NPC.
- Published
- 1996
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