1. Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry
- Author
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Wolfgang Schellenberger, Arne C. Rodloff, Christiane Rosenkranz, Reiner Schaumann, Klaus Eschrich, Kevin Losensky, Nicolas Knoop, Gelimer H. Genzel, and Catalina Suzana Stingu
- Subjects
EPEC ,Serotype ,medicine.medical_specialty ,General Immunology and Microbiology ,biology ,E. coli ,Shigella sonnei ,biology.organism_classification ,medicine.disease_cause ,Mass spectrometry ,Enterobacteriaceae ,Article ,Microbiology ,Matrix-assisted laser desorption/ionization ,Medical microbiology ,Salmonella enterica ,medicine ,EHEC ,MALDI-TOF MS ,Salmonella spp ,Escherichia coli - Abstract
Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry Matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) has proven to be an effective identification tool in medical microbiology. Discrimination to subspecies or serovar level has been found to be challenging using commercially available identification software. By forming our own reference database and using alternative analysis methods, we could reliably identify all implemented Enterobacteriaceae and non-fermenting gram negative bacilli by MALDI-TOF MS and even succeeded to distinguish Shigella sonnei from Escherichia coli (E. coli) and Salmonella enterica spp. enterica serovar Enteritidis from Salmonella enterica spp. enterica serovar Typhimurium. Furthermore, the method showed the ability to separate Enterohemorrhagic E. coli (EHEC) and Enteropathogenic E. coli (EPEC) from non-enteropathogenic E. coli.
- Published
- 2013
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