1. FLOT1 promotes tumor development, induces epithelial–mesenchymal transition, and modulates the cell cycle by regulating the Erk/Akt signaling pathway in lung adenocarcinoma.
- Author
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Zhang, Louqian, Mao, Yuan, Mao, Qixing, Fan, Weifei, Xu, Li, Chen, Yan, Xu, Lin, and Wang, Jun
- Subjects
ADENOCARCINOMA ,CANCER treatment ,TREATMENT of lung tumors ,CELL proliferation ,APOPTOSIS ,CANCER invasiveness ,CELL cycle ,CELL motility ,CELLULAR signal transduction ,EPITHELIAL cells ,GENE expression ,MEMBRANE proteins ,RETROVIRUSES ,TRANSFERASES ,TUMOR markers ,DISEASE progression - Abstract
Background: FLOT1 is a scaffolding protein of lipid rafts that is believed to be involved in numerous cellular processes. However, few studies have explored the function of FLOT1 in the development of lung adenocarcinoma (LUAD) and the underlying mechanisms of FLOT1 activity. Methods: FLOT1 knockdown and overexpression models were constructed via lentivirus. Cell growth, invasion, migration, and apoptosis were detected to evaluate the role of FLOT1 in LUAD development. Epithelial–mesenchymal transition (EMT) and cell cycle regulatory markers were then examined. Finally, the influence of FLOT1 on the Erk/Akt signaling pathway was investigated. Results: FLOT1 promoted cell growth, invasion, and migration and inhibited cell apoptosis. In addition, FLOT1 induced EMT and modulated the cell cycle by activating the Erk/Akt signaling pathway. Conclusion: The findings indicate a significant role of FLOT1 in LUAD development. Targeting FLOT1 may be a potential therapeutic strategy for LUAD. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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