1. Two Genes Homologous with Human Protein S cDNA Are Located on Chromosome 3
- Author
-
Egbert Bakker, Pieter H. Reitsma, R M Bertina, van der Zanden Al, and Ploos van Amstel Jk
- Subjects
biology ,cDNA library ,Hematology ,Molecular biology ,Restriction fragment ,Chromosome 17 (human) ,chemistry.chemical_compound ,Chromosome 3 ,chemistry ,Complementary DNA ,biology.protein ,Chromosome 22 ,Gene ,DNA - Abstract
SummaryA cDNA coding for the carboxy-terminal region of human protein S and containing a complete 3’-untranslated region, was isolated by a combination of antibody screening of a λgt11 human liver cDNA expression library and in situ hybridization of a pUC9 human liver cDNA library.Hybridization analysis of human total DNA with radiolabelled non-overlapping cDNA restriction fragments revealed the existence of two genes per haploid genome homologous with the protein S cDNA. Both genes were mapped to chromosome 3 using human-rodent cell hybrids. Neither of the genes showed polymorphism for sixteen different enzymes upon hybridization with the protein S cDNA.
- Published
- 1987
- Full Text
- View/download PDF