1. Serological and molecular detection of Theileria equi and Babesia caballi in Philippine horses.
- Author
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Ybañez AP, Ybañez RHD, Talle MG, Arreglo RMT, Geens MJC, Villas JGI 3rd, Villar SR, Laruga CL, Cao S, Moumouni FPA, Liu M, Igarashi I, and Xuan X
- Subjects
- Animals, Babesia isolation & purification, Babesiosis diagnosis, Babesiosis epidemiology, Babesiosis parasitology, Chromatography, Affinity, DNA, Protozoan genetics, Horse Diseases blood, Horse Diseases diagnosis, Horse Diseases parasitology, Horses, Philippines epidemiology, Polymerase Chain Reaction veterinary, Serologic Tests, Theileria isolation & purification, Theileriasis diagnostic imaging, Theileriasis epidemiology, Theileriasis parasitology, Ticks parasitology, Babesia genetics, Babesia immunology, Babesiosis blood, Horse Diseases epidemiology, Theileria genetics, Theileria immunology, Theileriasis blood
- Abstract
Theileria equi and Babesia caballi are tick-borne protozoan parasites that can cause anemia in horses. In the Philippines, serological detection of these parasites has only been reported in the Northern area (Luzon). In this study, 105 horses from Cebu and Bohol, Philippines were tested using peripheral blood smear examination (PBSE), immunochromatographic test (ICT) strips, and PCR. Clinical history, presenting clinical signs and complete blood count were obtained. Results revealed that although all horses were negative using PBSE, 23 (21.9%) were positive (12 for T. equi, and 11 for B. caballi) using ICT. PCR revealed 26 and 2 horses positive for T. equi and B. caballi, respectively. All positive horses showed no clinical signs. Partial DNA sequences of representative amplicons were found 100% identical to GenBank registered T. equi and B. caballi sequences. Statistical analyses revealed that location was found associated with T. equi PCR positivity and B. caballi seropositivity. This study documents the first serological detection of T. equi and B. caballi in horses in the southern area of the Philippines, and their first molecular detection and characterization in the country., (Copyright © 2018 Elsevier GmbH. All rights reserved.)
- Published
- 2018
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