1. Antibodies directed against HLA-DR gene products exhibit the CYNAP phenomenon*
- Author
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Glenn E. Rodey, Thomas C. Fuller, John W. Oldfather, Robert W. Karr, and Howard M. Gebel
- Subjects
Genes, MHC Class II ,education ,Immunology ,Immune sera ,Biochemistry ,Antibodies ,Antigen-Antibody Reactions ,Antibody Specificity ,Genetics ,HLA-DR ,Humans ,Immunology and Allergy ,Gene ,HLA-DR Antigen ,Antiserum ,B-Lymphocytes ,biology ,Antigen-antibody reactions ,Immune Sera ,Histocompatibility Antigens Class II ,HLA-DR Antigens ,General Medicine ,Cytotoxicity Tests, Immunologic ,Virology ,Molecular biology ,Complement-dependent cytotoxicity ,biology.protein ,Antibody - Abstract
Two well characterized Eighth International Workshop sera, 8w1090 and 8w112 , described to have anti-DR3 and anti-DR5 activity, respectively, were tested by standard and antiglobulin augmented (AHG) complement dependent cytotoxicity (CDC) assays with 21 target cells. We demonstrate that both sera, in fact, have only one antibody (most likely anti-MT2), but have different CYNAP patterns. These observations are discussed with regard to a more complete analysis of antisera that detect gene products of the HLA-DR region.
- Published
- 2008
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