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Your search keyword '"Badylak, Stephen F."' showing total 18 results
Start Over Author "Badylak, Stephen F." Journal tissue engineering
18 results on '"Badylak, Stephen F."'

2. Contributors

Author

Aldana, Ana A., primary, Alexander, Morgan R., additional, Atala, Anthony, additional, Badylak, Stephen F., additional, Baker, Matthew B., additional, Bauer, Jurica, additional, Black, Cameron, additional, Bobbala, Sharan, additional, Brittberg, Mats, additional, Brook, Gary A., additional, Buchanan, Fraser, additional, Carlier, Aurélie, additional, Chen, Saray, additional, Claes, Evan, additional, Cohen, Smadar, additional, Connelly, John, additional, Dalby, Matthew J., additional, Dalton, Paul D., additional, Dawson, Jonathan I., additional, de Boer, Jan, additional, Desmet, Tim, additional, Donnelly, Hannah, additional, Donvil, Filip, additional, Dziki, Jenna L., additional, Egger, Dominik, additional, Filippi, Miriam, additional, Gensler, Marius, additional, Gibbs, David, additional, Gibbs, Susan, additional, Hannen, Rosalind, additional, Hansmann, Jan, additional, Harvey, Alan R., additional, Heck, Tommy, additional, Herrmann, Marietta, additional, Hook, Andrew L., additional, Hubbell, Jeffrey A., additional, Hutmacher, Dietmar W., additional, Hynes, Clara Grace, additional, Joly, Johan, additional, Jorgensen, Adam M., additional, Kanczler, Janos, additional, Karperien, Marcel, additional, Kasper, Cornelia, additional, Kerr, Candace L., additional, Kilian, Kristopher A., additional, Kreß, Sebastian, additional, LaPointe, Vanessa, additional, Laschke, Matthias W., additional, Lindahl, Anders, additional, Londono, Ricardo, additional, Luyten, Frank P., additional, Marechal, Marina, additional, Martino, Mikaël M., additional, Moos, Malcolm, additional, Moroni, Lorenzo, additional, Morra, Emily, additional, Myers, Simon, additional, Nebel, Sabrina, additional, Nie, Minghao, additional, Nisbet, David R., additional, O'Neill, Kelly L., additional, Ojeh, Nkemcho, additional, Oreffo, Richard OC., additional, Oudega, Martin, additional, Passier, Robert, additional, Pêgo, Ana Paula, additional, Pittenger, Mark F., additional, Plant, Giles W., additional, Rice, Jeffrey J., additional, Roelen, Bernard A.J., additional, Schaschkow, Anaïs, additional, Scherberich, Arnaud, additional, Schrooten, Jan, additional, Scott, Evan A., additional, Sicari, Brian M., additional, Sonnaert, Maarten, additional, Später, Thomas, additional, Takeuchi, Shoji, additional, Tandon, Biranche, additional, Tare, Rahul, additional, Truckenmüller, Roman, additional, Tsimbouri, Monica, additional, Uquillas, Jorge Alfredo, additional, Van Assche, Dieter, additional, Vermeulen, Steven, additional, Verrier, Sophie, additional, Walsh, Pamela, additional, and Winkler, David A., additional

Published
2023
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4. Contributors

Author

Asnaghi, M. Adelaide, primary, Aerts, Jean-Marie, additional, Alakpa, Enateri V., additional, Alexander, Morgan R., additional, Alini, Mauro, additional, Alm, Jessica J., additional, Atala, Anthony, additional, Badylak, Stephen F., additional, Groot, Florence Barrère-de, additional, Black, Cameron, additional, Brittberg, Mats, additional, Cha, Chaenyung, additional, Cohen, Smadar, additional, Dalby, Matthew J., additional, Dalton, Paul D., additional, Davison, Noel L., additional, Dawson, Jonathan I., additional, de Boer, Jan, additional, DeRuiter, Marco C., additional, Engler, Adam J., additional, Geris, Liesbet, additional, Gibbs, Susan, additional, Gibbs, David, additional, Gittenberger-de Groot, Adriana C., additional, Grijpma, Dirk W., additional, Han, Chungmin, additional, Harvey, Alan R., additional, Herrmann, Marietta, additional, Hierck, Beerend P., additional, Hook, Andrew L., additional, Hubbell, Jeffrey A., additional, Hutmacher, Dietmar W., additional, Joly, Johan, additional, Kanczler, Janos, additional, Karperien, Marcel, additional, Kerr, Candace, additional, Khademhosseini, Ali, additional, Labuz, Joseph M., additional, Lambrechts, Toon, additional, LaPointe, Vanessa L.S., additional, Laschke, Matthias W., additional, Le Blanc, Katarina, additional, Levenberg, Shulamit, additional, Lindahl, Anders, additional, Londono, Ricardo, additional, Luyten, Frank P., additional, Mantalaris, Athanasios, additional, Martin, Ivan, additional, Martino, Mikaël M., additional, McNamara, Laura E., additional, Meijer, Dies, additional, Moroni, Lorenzo, additional, Myers, Simon, additional, Navsaria, Harshad, additional, Ojeh, Nkemcho, additional, Oreffo, Richard O.C., additional, Oudega, Martin, additional, Papantoniou, Ioannis, additional, Park, Jaesung, additional, Piraino, Francesco, additional, Pittenger, Mark F., additional, Plant, Giles W., additional, Poelmann, Rob E., additional, Qian, Hong, additional, Reischl, Ilona, additional, Rice, Jeffrey J., additional, Roelen, Bernard A.J., additional, Rouwkema, Jeroen, additional, Ruvinov, Emil, additional, Sandberg, Jason M., additional, Scherberich, Arnaud, additional, Schrooten, Jan, additional, Scott, Evan A., additional, Shandalov, Yulia, additional, Sicari, Brian M., additional, Smith, Timothy, additional, Sohier, Jérôme, additional, Sonnaert, Maarten, additional, Takayama, Shuichi, additional, Tare, Rahul, additional, Truckenmüller, Roman, additional, Tsimbouri, Penelope M., additional, Turner, Lesley-Anne, additional, Van Assche, Dieter, additional, van Blitterswijk, Clemens A., additional, Vanlauwe, Johan, additional, Verrier, Sophie, additional, Welin, Stellan, additional, Wendt, David, additional, Winkler, Dave A., additional, and Woodfield, Tim B.F., additional

Published
2015
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17. The Basement Membrane Component of Biologic Scaffolds Derived from Extracellular Matrix

Author

Brown, Bryan, Lindberg, Kristina, Reing, Janet, Stolz, Donna Beer, and Badylak, Stephen F.

Abstract

The extracellular matrix (ECM) has been successfully used as a scaffold for constructive remodeling of multiple tissues in both preclinical studies and in human clinical applications. The basement membrane is a specialized form of the ECM that supports and facilitates the growth of epithelial cell populations. The morphology and the molecular composition of the ECM, including the basement membrane, vary depending upon the organ from which the ECM is harvested and the methods by which it is processed for use as a medical device. Processing steps, such as decellularization, lyophilization, disinfection, and terminal sterilization, may affect the morphology and composition of an ECM scaffold, including, but not limited to, the integrity of a basement membrane complex. The present study evaluated the presence and integrity of a basement membrane complex in processed ECM derived from three different tissues: the urinary bladder, small intestine, and liver. Immunohistochemical determination of the presence and localization of three basement membrane molecules, collagen IV, laminin, and collagen VII, was conducted for each ECM scaffold. Scanning electron microscopy (SEM) was used to further explore the surface ultrastructure of selected ECM scaffolds. The effect of a surface basement membrane presence upon the pattern of in vitro growth of two separate cell types, NIH 3T3 fibroblasts and human microvascular endothelial cells (HMEC), was also evaluated for each ECM scaffold. Results showed that the only intact basement membrane complex was found on the luminal surface of the ECM derived from the urinary bladder and that the basement membrane was an effective barrier to penetration of the scaffold by the seeded cells. We conclude that the urinary bladder ECM but not the small intestine- or liver-derived ECM contains a surface with composition and morphology consistent with that of an intact basement membrane complex, that the basement membrane complex can survive processing, and that the basement membrane structure can modulate in vitro cell growth patterns.

Published
2006
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18. Maintenance of hepatic sinusoidal endothelial cell phenotype in vitro using organ-specific extracellular matrix scaffolds.

Author

Sellaro TL, Ravindra AK, Stolz DB, and Badylak SF

Subjects
Animals, Animals, Genetically Modified, Cells, Cultured, Phenotype, Rats, Rats, Sprague-Dawley, Biocompatible Materials, Endothelial Cells cytology, Extracellular Matrix, Liver cytology, Tissue Engineering instrumentation
Abstract

Sinusoidal endothelial cells (SECs) are notoriously difficult to culture in vitro. SECs represent a highly specialized endothelial cell (EC) population, and traditional methods of SEC isolation from the liver initiate a process of SEC dedifferentiation. Acellular extracellular matrix (ECM) scaffolds were investigated in a physiologically relevant in vitro culture model for their ability to maintain SEC phenotype. The cell culture model used SECs only or a coculture of SECs with hepatocytes on ECM substrates derived from the liver (L-ECM), bladder (UBM-ECM), or small intestine submucosa (SIS-ECM). The effect of the ECM substrate upon SEC dedifferentiation was evaluated using scanning electron microscopy (SEM) and confocal microscopy. When SECs alone were cultured on uncoated glass slides, collagen I, UBM-ECM, or SIS-ECM, SECs showed signs of dedifferentiation after 1 day. In contrast, SECs alone cultured on L-ECM maintained their differentiated phenotype for at least 3 days, indicated by the presence of many fenestrations on SEC surface, expression of anti-rat hepatic sinusoidal endothelial cells mouse IgG MoAb (SE-1), and lack of expression of CD31. When SECs were cocultured with hepatocytes on any of the ECM scaffolds, the SECs maintained a near-normal fenestrated phenotype for at least 1 day. However, SEM revealed that the shape, size, frequency, and organization of the fenestrations varied greatly depending on ECM source. At all time points, SECs cocultured with hepatocytes on L-ECM maintained the greatest degree of differentiation. The present study demonstrated that the acellular ECM scaffold derived from the liver maintained SEC differentiation in culture longer than any of the tested substrate materials. The replacement of complex tissues and 3-dimensional organs may require specialized scaffolds to support multiple, functional cell phenotypes.

Published
2007
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