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Your search keyword '"Giribaldi, G"' showing total 3 results
Start Over Author "Giribaldi, G" Journal toxicology and applied pharmacology
3 results on '"Giribaldi, G"'

1. Dextran-shelled oxygen-loaded nanodroplets reestablish a normoxia-like pro-angiogenic phenotype and behavior in hypoxic human dermal microvascular endothelium

Author

Ilaria Rivolta, Monica Argenziano, Marco Soster, Giulia Rossana Gulino, Giuliana Giribaldi, Amina Khadjavi, C. Magnetto, Tullio Genova, Sarah D'Alessandro, Mauro Prato, Roberta Cavalli, Nicoletta Basilico, Alice Panariti, Caterina Guiot, Basilico, N, Magnetto, C, D'Alessandro, S, Panariti, A, Rivolta, I, Genova, T, Khadjavi, A, Gulino, G, Argenziano, M, Soster, M, Cavalli, R, Giribaldi, G, Guiot, C, and Prato, M

Subjects
Keratinocytes, Nanostructure, Matrix metalloproteinase, Toxicology, Extracellular matrix, Anoxia, Hypoxia, Skin, education.field_of_study, Endothelial Cell, Tissue inhibitor of metalloproteinase (TIMP), Medicine (all), Proteolytic enzymes, Cell migration, Dextrans, Cell biology, Human microvascular endothelial cell (HMEC), Matrix metalloproteinase (MMP), Nanodroplet, Oxygen, Angiogenesis Inducing Agents, Cell Line, Cell Survival, Chitosan, Endothelial Cells, Endothelium, Vascular, Extracellular Matrix, Gelatinases, Humans, Matrix Metalloproteinase 2, Matrix Metalloproteinase 9, Nanostructures, Phenotype, Tissue Inhibitor of Metalloproteinase-1, Tissue Inhibitor of Metalloproteinase-2, Wound Healing, Pharmacology, medicine.anatomical_structure, Gelatinase, medicine.symptom, Keratinocyte, Human, Endothelium, Population, Angiogenesis Inducing Agent, Biology, Vascular, medicine, education, Dextran, Hypoxia (medical), Immunology, Wound healing
Abstract

In chronic wounds, hypoxia seriously undermines tissue repair processes by altering the balances between pro-angiogenic proteolytic enzymes (matrix metalloproteinases, MMPs) and their inhibitors (tissue inhibitors of metalloproteinases, TIMPs) released from surrounding cells. Recently, we have shown that in human monocytes hypoxia reduces MMP-9 and increases TIMP-1 without affecting TIMP-2 secretion, whereas in human keratinocytes it reduces MMP-2, MMP-9, and TIMP-2, without affecting TIMP-1 release. Provided that the phenotype of the cellular environment is better understood, chronic wounds might be targeted by new oxygenating compounds such as chitosan- or dextran-shelled and 2H,3H-decafluoropentane-cored oxygen-loaded nanodroplets (OLNs). Here, we investigated the effects of hypoxia and dextran-shelled OLNs on the pro-angiogenic phenotype and behavior of human dermal microvascular endothelium (HMEC-1 cell line), another cell population playing key roles during wound healing. Normoxic HMEC-1 constitutively released MMP-2, TIMP-1 and TIMP-2 proteins, but not MMP-9. Hypoxia enhanced MMP-2 and reduced TIMP-1 secretion, without affecting TIMP-2 levels, and compromised cell ability to migrate and invade the extracellular matrix. When taken up by HMEC-1, nontoxic OLNs abrogated the effects of hypoxia, restoring normoxic MMP/TIMP levels and promoting cell migration, matrix invasion, and formation of microvessels. These effects were specifically dependent on time-sustained oxygen diffusion from OLN core, since they were not achieved by oxygen-free nanodroplets or oxygen-saturated solution. Collectively, these data provide new information on the effects of hypoxia on dermal endothelium and support the hypothesis that OLNs might be used as effective adjuvant tools to promote chronic wound healing processes.

Published
2015

2. Dextran-shelled oxygen-loaded nanodroplets reestablish a normoxia-like pro-angiogenic phenotype and behavior in hypoxic human dermal microvascular endothelium.

Author

Basilico N, Magnetto C, D'Alessandro S, Panariti A, Rivolta I, Genova T, Khadjavi A, Gulino GR, Argenziano M, Soster M, Cavalli R, Giribaldi G, Guiot C, and Prato M

Subjects
Angiogenesis Inducing Agents chemistry, Cell Line, Cell Survival drug effects, Chitosan chemistry, Chitosan pharmacology, Dextrans chemistry, Endothelial Cells drug effects, Endothelial Cells metabolism, Endothelium, Vascular cytology, Endothelium, Vascular metabolism, Extracellular Matrix drug effects, Extracellular Matrix metabolism, Gelatinases metabolism, Humans, Keratinocytes drug effects, Keratinocytes metabolism, Matrix Metalloproteinase 2 metabolism, Matrix Metalloproteinase 9 metabolism, Oxygen chemistry, Phenotype, Skin drug effects, Skin metabolism, Tissue Inhibitor of Metalloproteinase-1 metabolism, Tissue Inhibitor of Metalloproteinase-2 metabolism, Wound Healing drug effects, Angiogenesis Inducing Agents pharmacology, Dextrans pharmacology, Endothelium, Vascular drug effects, Hypoxia drug therapy, Nanostructures chemistry, Oxygen pharmacology
Abstract

In chronic wounds, hypoxia seriously undermines tissue repair processes by altering the balances between pro-angiogenic proteolytic enzymes (matrix metalloproteinases, MMPs) and their inhibitors (tissue inhibitors of metalloproteinases, TIMPs) released from surrounding cells. Recently, we have shown that in human monocytes hypoxia reduces MMP-9 and increases TIMP-1 without affecting TIMP-2 secretion, whereas in human keratinocytes it reduces MMP-2, MMP-9, and TIMP-2, without affecting TIMP-1 release. Provided that the phenotype of the cellular environment is better understood, chronic wounds might be targeted by new oxygenating compounds such as chitosan- or dextran-shelled and 2H,3H-decafluoropentane-cored oxygen-loaded nanodroplets (OLNs). Here, we investigated the effects of hypoxia and dextran-shelled OLNs on the pro-angiogenic phenotype and behavior of human dermal microvascular endothelium (HMEC-1 cell line), another cell population playing key roles during wound healing. Normoxic HMEC-1 constitutively released MMP-2, TIMP-1 and TIMP-2 proteins, but not MMP-9. Hypoxia enhanced MMP-2 and reduced TIMP-1 secretion, without affecting TIMP-2 levels, and compromised cell ability to migrate and invade the extracellular matrix. When taken up by HMEC-1, nontoxic OLNs abrogated the effects of hypoxia, restoring normoxic MMP/TIMP levels and promoting cell migration, matrix invasion, and formation of microvessels. These effects were specifically dependent on time-sustained oxygen diffusion from OLN core, since they were not achieved by oxygen-free nanodroplets or oxygen-saturated solution. Collectively, these data provide new information on the effects of hypoxia on dermal endothelium and support the hypothesis that OLNs might be used as effective adjuvant tools to promote chronic wound healing processes., (Copyright © 2015 Elsevier Inc. All rights reserved.)

Published
2015
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3. Chitosan-shelled oxygen-loaded nanodroplets abrogate hypoxia dysregulation of human keratinocyte gelatinases and inhibitors: New insights for chronic wound healing.

Author

Khadjavi A, Magnetto C, Panariti A, Argenziano M, Gulino GR, Rivolta I, Cavalli R, Giribaldi G, Guiot C, and Prato M

Subjects
Cell Hypoxia drug effects, Cell Hypoxia physiology, Cell Line, Cell Survival drug effects, Cell Survival physiology, Chitosan chemistry, Drug Carriers administration & dosage, Drug Carriers chemistry, Enzyme Inhibitors pharmacology, Gelatinases metabolism, Humans, Keratinocytes enzymology, Male, Middle Aged, Nanoparticles chemistry, Oxygen chemistry, Wound Healing physiology, Chitosan administration & dosage, Gelatinases antagonists & inhibitors, Keratinocytes drug effects, Nanoparticles administration & dosage, Oxygen administration & dosage, Wound Healing drug effects
Abstract

Background: In chronic wounds, efficient epithelial tissue repair is hampered by hypoxia, and balances between the molecules involved in matrix turn-over such as matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) are seriously impaired. Intriguingly, new oxygenating nanocarriers such as 2H,3H-decafluoropentane-based oxygen-loaded nanodroplets (OLNs) might effectively target chronic wounds., Objective: To investigate hypoxia and chitosan-shelled OLN effects on MMP/TIMP production by human keratinocytes., Methods: HaCaT cells were treated for 24h with 10% v/v OLNs both in normoxia or hypoxia. Cytotoxicity and cell viability were measured through biochemical assays; cellular uptake by confocal microscopy; and MMP and TIMP production by enzyme-linked immunosorbent assay or gelatin zymography., Results: Normoxic HaCaT cells constitutively released MMP-2, MMP-9, TIMP-1 and TIMP-2. Hypoxia strongly impaired MMP/TIMP balances by reducing MMP-2, MMP-9, and TIMP-2, without affecting TIMP-1 release. After cellular uptake by keratinocytes, nontoxic OLNs abrogated all hypoxia effects on MMP/TIMP secretion, restoring physiological balances. OLN abilities were specifically dependent on time-sustained oxygen diffusion from OLN core., Conclusion: Chitosan-shelled OLNs effectively counteract hypoxia-dependent dysregulation of MMP/TIMP balances in human keratinocytes. Therefore, topical administration of exogenous oxygen, properly encapsulated in nanodroplet formulations, might be a promising adjuvant approach to promote healing processes in hypoxic wounds., (Copyright © 2015 Elsevier Inc. All rights reserved.)

Published
2015
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