1. CHARACTERIZATION OF PURIFIED MS2 BACTERIOPHAGE BY THE PHYSICAL COUNTING METHODOLOGY USED IN THE INTEGRATED VIRUS DETECTION SYSTEM (IVDS).
- Author
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Wick, Charles H. and McCubbin, Patrick E.
- Subjects
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BACTERIOPHAGES , *DETECTION of microorganisms , *VIRUS identification - Abstract
A new physically based methodology-the integrated virus detection system (IVDS)-was used to characterize a high-concentration, 10.2 mg protein/ mL, sample preparation of MS2 bacteriophage with a reported 10[sup 14] plaque-forming units (pfu)/mL (DPM14) virus count in a common TNME buffer. Virus counts were made using the IVDS instrument following serial dilution. Results indicated virus counts of 1.5 x 10[sup 5] for the neat sample (DPM14), followed by 6.5 x 10[sup 4] viruses (DPM13),1.2 x 10[sup 4] viruses (DPM12),9.3 x 10[sup 2] viruses (DPM11), 88 viruses (DPM10), and 5 viruses (DPM9), respectively. Lower concentrations displayed a consistent multiplier and were consistent with target dilutions. Increases in virus concentration appear to decrease the multiplier, probably through aggregation. The results demonstrate a consistent and simple-to-use methodology. The results further indicate that the IVDS instrument can be used for characterization of other virus preparations with equal ease and similar results. [ABSTRACT FROM AUTHOR] more...
- Published
- 1999
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