Search

Background: Anti‐K is an alloantibody stimulated in response to the KEL1 antigen and may cause hemolytic disease of the fetus and newborn (HDFN). Provision of KEL1 negative blood to females of child‐bearing potential was not our practice. We assessed the impact of our policy and assessed feasibility of a KEL1 negative transfusion policy. Study design and methods: This is a cohort study spanning Jan 1, 2007–Jun 30, 2017 in Hamilton, Canada. Data were obtained via our institution's transfusion database. Chart reviews of females age ≤45 with anti‐K were performed; data on RBC KEL1 phenotype were obtained from the blood supplier when needed to ascertain the cause of alloimmunization. Descriptive analysis of hospital KEL1 negative inventory demand and supply was performed. Results: From Jan 2007‐Jun 2017, 8.6% of all RBC units transfused were provided to females age ≤45. There were 111 females with detectable anti‐K. Median age at time of antibody detection was 34 years (interquartile range 27–40) and 28 of 111 (25.2%) patients may have been alloimmunized by transfusion. Of 49 pregnancies, seven had complications due to anti‐K. We estimated that our existing RBC inventory (with 16% units known to be KEL1 negative in 2017) is sufficient to meet demand and support a KEL1 negative transfusion policy for females age ≤45. Conclusion: Transfusion was responsible for alloimmunization in 25% of females with anti‐K over 10 years. Analysis of supply and demand can be used to inform feasibility of a KEL1 negative transfusion policy. [ABSTRACT FROM AUTHOR]

A novel variant DO*A allele with a c.370delT mutation leading to a DO-null phenotype in a Syrian family The Dombrock antigens are clinically relevant blood group antigens, known for potentially causing delayed and acute hemolytic transfusion reactions, although no hemolytic disease of the fetus and newborn has been described.1,2 We report here a new DO allele in a family of Syrian origin caused by a c.370delT (p.Leu124Cys) nonsense mutation, leading to a DO-null phenotype (ISBT Allele Number I DO*01 N.05 i , GenBank Accession Number MT747635). In this report, we describe a novel I DO*A i allele (c.370delT [p.Leu124Cysfs*4]) involving a nonsense mutation, leading to absent expression of all Dombrock antigens or a DO-null phenotype. [Extracted from the article]