5 results on '"Tazzari PL"'
Search Results
2. Patients with neoplastic and nonneoplastic hematologic diseases acquire CTLA-4 antibodies after blood transfusion.
- Author
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Pistillo MP, Tazzari PL, Gaudiano C, Cilla V, Kato T, Matsui T, Nishioka K, Capanni P, Conte R, and Ferrara GB
- Subjects
- Abatacept, Adolescent, Adult, Aged, Antibodies blood, Antibody Specificity, Antigens, CD, Antigens, Differentiation blood, CTLA-4 Antigen, Epitope Mapping, Female, Hematologic Diseases blood, Humans, Male, Middle Aged, Recombinant Fusion Proteins immunology, Transfusion Reaction, Antibodies immunology, Antigens, Differentiation immunology, Blood Transfusion, Hematologic Diseases immunology, Hematologic Diseases therapy, Immunoconjugates
- Abstract
Background: The presence of antibodies to CTLA-4, a negative regulator of T-cell activation, was investigated in multiply transfused patients with malignant and non- malignant hematologic diseases. A previous study showed that, in multiply transfused patients, an immune response against nuclear matrix proteins can be induced by WBCs undergoing apoptosis during RBC unit storage. This study evaluated whether the same phenomenon could be involved in the induction of CTLA-4 antibodies in the patients analyzed., Study Design and Methods: Patient sera were tested for binding to the recombinant full-length CTLA-4 beta-galactosidase fusion protein by an ELISA. Immuno-fluorescence stainings were performed to analyze the CTLA-4 epitopes recognized by the antibodies and to detect such epitopes in the apoptotic cells present in the RBC units., Results: CTLA-4 antibodies were found in multiply transfused patients with beta-thalassemia (40%) and with other hemolytic diseases (33%) including leukemias (42%). A higher incidence of CTLA-4 antibodies was found in patients receiving non-WBC-reduced blood (88%) than in those receiving WBC-reduced blood (26%). Immunofluorescence staining showed that WBCs undergoing apoptosis in the RBC unit expressed CTLA-4 epitopes., Conclusions: The apoptotic WBCs present in the RBC units, after cold storage, express CTLA-4 epitopes. These epitopes can be released and induce formation of CTLA-4 antibodies with profound implications in the development of autoimmune disorders and in facilitating tumor dissemination and metastasis.
- Published
- 2001
- Full Text
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3. Nuclear matrix protein is released from apoptotic white cells during cold (1-6 degrees C) storage of concentrated red cell units and might induce antibody response in multiply transfused patients.
- Author
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Martelli AM, Tazzari PL, Bortul R, Riccio M, Tabellini G, Santi S, Frabetti F, Musiani D, Bareggi R, and Conte R
- Subjects
- Antibodies blood, Antibody Formation, Antigens, Nuclear, Apoptosis immunology, Cold Temperature, Coloring Agents, Flow Cytometry, Fluorescent Antibody Technique, Humans, Immunoblotting, Microscopy, Confocal, Blood Preservation methods, Blood Transfusion, Erythrocytes chemistry, Leukocytes cytology, Nuclear Proteins immunology, Nuclear Proteins metabolism
- Abstract
Background: A previous study showed that white cells in blood units undergo apoptosis during storage., Study Design and Methods: The present study attempts to show the release of nuclear matrix protein (NMP) in the supernatants of red cell units and to determine whether antibodies against nuclear components may be present in multiply transfused patients; the methods employed were enzyme-linked immunosorbent assay, flow cytometry, microscopy, immunoblotting, immunofluorescence, and confocal laser-scanning microscopy., Results: NMP is released from white cells in the supernatant of packed red cell units upon cold storage (1-6 degrees C). The concentration of NMP correlates well with the degree of apoptosis, as analyzed by flow cytometry, nuclear dye staining, and DNA gel electrophoresis. Immunofluorescence also shows that white cells undergoing apoptosis (pre-G(1) peak, as seen by propidium iodide staining and flow cytometry) have an NMP content lower than control cells, which confirms an actual release of NMP. Moreover, immunoblotting analysis and immunofluorescent staining showed that, in 4 of 38 multiply transfused patients, autoantibodies against NMPs were present without any clinical or laboratory sign of autoimmune disease. One of the sera, recognizing a 64-kDa NMP, immunostained nuclear dots that were identified as coiled bodies because of their colocalization with p 80 coilin., Conclusion: NMP is released in the supernatant of red cell units. The results obtained from patients suggest that nuclear proteins released during apoptosis, once transfused, may induce an immune response in multiply transfused patients.
- Published
- 2000
- Full Text
- View/download PDF
4. White cell apoptosis in platelet concentrates.
- Author
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Frabetti F, Tazzari PL, Musiani D, Bontadini A, Matteini C, Roseti L, Tassi C, Viggiani M, Marini M, and Conte R
- Subjects
- Cell Membrane, Coloring Agents, Cytokines metabolism, Flow Cytometry, Humans, Platelet Activation, Plateletpheresis, Apoptosis, Blood Platelets cytology, Leukocytes cytology
- Abstract
Background: The aim of the present study was the evaluation of the apoptosis in residual white cells (WBCs) contained in platelet concentrates (PCs) and of the relationship of this apoptosis with the concentration of inflammatory cytokines in the medium and with platelet activation., Study Design and Methods: Three independent methods were used to evaluated apoptosis in WBCs present in 9 PCs, either from single donors by apheresis (SD-PCs) or from pooled buffy coats (BC-PCs). All PCs were divided in two parts, one of which was irradiated. PCs were stored up to 4 days at room temperature, and samples were withdrawn daily for analysis of apoptosis, of platelet activation (surface and soluble CD62P), and of cytokine concentration (interleukin [IL]-1alpha, IL-1beta, IL-6, IL-8, and tumor necrosis factor alpha)., Results: Apoptosis was found to occur with storage in both irradiated and nonirradiated units. Platelet activation increased with storage time and was higher in BC-PCs. The amount of released cytokines was rather variable among PC units. Only IL-8 was consistently found to increase with storage time., Conclusions: Apoptosis of residual WBCs occurred in PC units as a function of storage time. The amount and the time course of apoptosis seem to correlate with IL-8 release rather than with platelet activation or with the occurrence of febrile nonhemolytic transfusion reactions.
- Published
- 2000
- Full Text
- View/download PDF
5. White cell apoptosis in packed red cells.
- Author
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Frabetti F, Musiani D, Marini M, Fanelli C, Coppola S, Ghibelli L, Tazzari PL, Bontadini A, Tassi C, and Conte R
- Subjects
- Adenine pharmacology, Anticoagulants pharmacology, Apoptosis drug effects, Apoptosis physiology, Apoptosis radiation effects, Blood Preservation, Citrates pharmacology, DNA Fragmentation radiation effects, Flow Cytometry, Glucose pharmacology, Humans, Leukocytes cytology, Lymphocyte Activation, Phosphates pharmacology, Temperature, Time Factors, Erythrocytes, Leukocytes physiology
- Abstract
Background: After the removal of the buffy coat, packed red cell (RBC) transfusion units still contain white cells that may undergo apoptosis as a result of storage conditions (1-6 degrees C). The aim of the present study was the evaluation of this phenomenon in view of the possible influence it may have on febrile nonhemolytic transfusion reactions., Study Design and Methods: Three independent methods (microscopy, DNA electrophoresis, and cytometry) were used to evaluate apoptosis in white cells present in 13 RBC units. Of these units, 10 had been collected into CPD/saline-adenine-glucose-mannitol and 3 into CPDA-1; each bag was split in two parts, one of which was irradiated. RBCs were stored at 1 to 6 degrees C, and samples were periodically withdrawn for study. The proliferative capacity of stored lymphocytes was evaluated after phytohemagglutinin stimulation and tritiated thymidine incorporation., Results: Apoptosis was found to occur in both granulocytes and lymphocytes, starting from the first 48 to 72 hours of storage. The choice of the anticoagulant-preservative solution and the effect of irradiation did not influence the amount and the timing of the apoptotic phenomenon. Lymphocyte proliferative capacity was found to decrease sharply with storage time., Conclusion: Conditions of storage in RBCs induce consistent apoptosis in residual white cells. The possible clinical implications of the relationships between apoptosis and the induction of biologic response modifiers (that may cause interleukin-mediated febrile non-hemolytic transfusion reactions) and between apoptosis and immune reactions remain to be elucidated.
- Published
- 1998
- Full Text
- View/download PDF
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