1. Determination of optimum levels of binding antibody units (BAU) of new quantitative chemiluminescent immuno-assay (CLIA) in COVID-19 vaccinated volunteer blood donors.
- Author
-
Tiwari, Aseem Kumar, Aggarwal, Geet, Kale, Pratibha, Yadav, Neeti, Kumar, Virendra, Singh, Gargi, Cheirmaraj, K., and Krishnan, K.
- Subjects
- *
BLOOD donors , *CHEMILUMINESCENCE assay , *RECEIVER operating characteristic curves , *VACCINE effectiveness , *IMMUNOGLOBULINS , *HEPATITIS B vaccines - Abstract
For assessment of COVID-19 vaccine efficacy, neutralization activity of anti-SARS-CoV-2 antibody is measured. This study was undertaken to determine optimum levels of binding antibody units (BAU/ml) in new quantitative chemiluminescent assay (CLIA) that corresponded to neutralizing potential (30% inhibition) of sVNT assay. Methods: Ninety-one blood samples were analyzed by CLIA and sVNT assays. Test samples (n = 75) were collected from blood donors post-2nd vaccination dose, while control samples (n = 16) were archived pre-COVID donor samples. Correlation between CLIA and sVNT was calculated and receiver operating characteristic (ROC) curve was drawn and analyzed. Results: Results indicated excellent correlation between 57.5 BAU/ml on CLIA and 30%inhibition on sVNT assay. ROC curve analysis revealed that the area under the curve (AUC) was 0.971. Discussion: The present study determined that 57.5 BAU/ml on CLIA corresponded to 30% inhibition on sVNT assay. Periodic quantitative analysis [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF