1. Engineering Robust Production Microbes for Large-Scale Cultivation
- Author
-
Wehrs, Maren, Tanjore, Deepti, Eng, Thomas, Lievense, Jeff, Pray, Todd R, and Mukhopadhyay, Aindrila
- Subjects
Biochemistry and Cell Biology ,Biological Sciences ,Industrial Biotechnology ,Bioengineering ,Bioreactors ,Fermentation ,Genetic Association Studies ,Microbiological Techniques ,Systems Biology ,biofuel ,bioproduct ,bioreactor ,heterogeneity ,microbial hosts ,mixing ,scale-up ,strain selection ,Microbiology ,Medical Microbiology ,Biochemistry and cell biology - Abstract
Systems biology and synthetic biology are increasingly used to examine and modulate complex biological systems. As such, many issues arising during scaling-up microbial production processes can be addressed using these approaches. We review differences between laboratory-scale cultures and larger-scale processes to provide a perspective on those strain characteristics that are especially important during scaling. Systems biology has been used to examine a range of microbial systems for their response in bioreactors to fluctuations in nutrients, dissolved gases, and other stresses. Synthetic biology has been used both to assess and modulate strain response, and to engineer strains to improve production. We discuss these approaches and tools in the context of their use in engineering robust microbes for applications in large-scale production.
- Published
- 2019