1. The roles of parafibromin expression in ovarian epithelial carcinomas: a marker for differentiation and prognosis and a target for gene therapy
- Author
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Xue-feng Yang, Wen-feng Gou, Xin Liu, Junjun Li, Shuai Shi, Shuang Zhao, Lei Fang, Dao-fu Shen, Hua-chuan Zheng, Xiang-xuan Zhao, Ji-cheng Wu, and Yang Gao
- Subjects
0301 basic medicine ,Adult ,Pathology ,medicine.medical_specialty ,Parafibromin ,Apoptosis ,Biology ,Carcinoma, Ovarian Epithelial ,medicine.disease_cause ,Metastasis ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Survivin ,medicine ,Biomarkers, Tumor ,Humans ,Neoplasms, Glandular and Epithelial ,Aged ,Aged, 80 and over ,Ovarian Neoplasms ,Tumor Suppressor Proteins ,Cell Differentiation ,General Medicine ,Genetic Therapy ,Cell cycle ,Middle Aged ,medicine.disease ,Prognosis ,Vascular endothelial growth factor ,030104 developmental biology ,chemistry ,030220 oncology & carcinogenesis ,Immunohistochemistry ,Female ,Ovarian cancer ,Carcinogenesis - Abstract
Parafibromin is a protein encoded by hyperparathyroidism 2 (HRPT2) and its downregulated expression is involved in the pathogenesis of parathyroid, breast, gastric, colorectal, lung, head and neck cancers. We aimed to investigate the roles of parafibromin expression in tumorigenesis, progression, or prognostic evaluation of ovarian cancers. HRPT2-expressing plasmid was transfected into ovarian cancer cells with the phenotypes and related molecules examined. The messenger RNA (mRNA) and protein expression of parafibromin were also examined in ovarian normal tissue, benign and borderline tumors and cancers by reverse transcription-polymerase chain reaction (RT-PCR), Western blot, or immunohistochemistry respectively. It was found that parafibromin overexpression caused a lower growth, migration and invasion, higher sensitivity to cisplatin and apoptosis than the mock and control (P
- Published
- 2015