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Introduction: The present investigation evaluated the effect of storage and packaging conditions on retention of ascorbic acid content of fenugreek leaves. Materials and methods: Fenugreek leaves (Trigonella Foenum- Graceum) with and without roots weighing 100 g were made and packed in perforated and non-perforated flexible consumer packets of 30 x 25 cm (750 sq cm) of four different types viz., LDPE -100 gauge, LDPE -200 gauge, PP-100 gauge and PP -200 gauge. For perforations 24 vents of 0.59 cm diameter were made covering an area of 1.87 sq. cm. These samples were stored at ambient (14-35° C, RH 50-60 per cent) and low temperature (0-5° C, RH 80-90 per cent) till acceptable. The samples were analyzed at regular intervals for ascorbic acid content using 2, 6 dichlorophenol indophenol method. Results and discussion: Fresh fenugreek leaves contained 51.4 mg/100g ascorbic acid. At lower temperature, fenugreek samples without root in non-perforated package (NPWORL) had a shelf life of six days and mean ascorbic acid retention of 80.95%. Packaging the samples with root (NPWRL) reduced the shelf life to four days period and retained 66.05% of ascorbic acid. Perforation in the packaging material also reduced the storage period to four days in both with (PWRL) and without root (PWORL) fenugreek samples. Fenugreek samples without and with roots stored at ambient temperature in non-perforated package (NPWORA and NPWRA) showed a maximum shelf life of two days with mean ascorbic acid retention of 69.99% and 56.47% respectively. A gradual decrease in the mean ascorbic acid content was observed at both low and ambient temperatures with the presence of roots and perforation in the packaging material. This decrease was observed to be significant at both low (p=0.004) and ambient (p= 0.055) temperatures. The ascorbic acid was also found to decrease with increase in days of storage at both low as well as ambient temperatures. The flexible packaging materials viz., LDPE and PP of 100 and 200 gauge did not show any significant difference in retention of ascorbic acid. Amongst interaction between perforations, roots, temperature, days of storage and packaging materials, the interactions between days of storage were found to be significant with perforations (p =0.023), root (p=0.053) and temperature (p= 0.00). Conclusions: Fenugreek samples stored without roots in nonperforated packaging material reflected significantly (p= 0.00) highest retention of ascorbic acid at low temperatures. [ABSTRACT FROM AUTHOR]