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• Feedforward comb filtering effect is proposed to extract the phase information of overlapped signals from immersed ultrasonic testing of coatings. • A quantitative relation between the notch frequency and interfacial stiffness is obtained from the numerical simulation. • Decimal part of the ratio of notch frequencies to interval frequencies is proposed as a novel index to evaluate the bonding strength of coatings. • A comparative study on bonding strength assessment is conducted based on ultrasonic nondestructive test and destructive cross-cut test. • A baseline-free ultrasonic nondestructive method is proposed for quantitative evaluation of bonding strength of coatings. Because of the ability to protect and decorate materials, polyurethane coatings (PU) are being applied more and more extensively in the transportation, furniture, automotive, and electronic industries. However, it is difficult to effectively evaluate the bonding quality of coatings by conventional destructive methods for high-volume products in industrial applications. In this work, an ultrasonic nondestructive method based on the feedforward comb filtering effect was proposed to assess the bonding strength of the PU coatings. The coating and substrate interface can be modeled as a distributed spring system. The phase of the reflected ultrasonic wave is highly related to the interfacial stiffness. The superimposed signals can be well analyzed based on the comb filtering effect, and an index called the ratio of notch frequencies to interval frequencies (NIR) was constructed to represent the phase difference. Finite element simulations were conducted to show the index's construction method and variation with different interfacial stiffnesses. Immersed ultrasonic testing experiments with a transducer of 39.43 MHz were then conducted on PU coatings on two kinds of substrates made of aluminum and magnesium alloys. Each specimen was scanned as a grid to mimic the procedure of the well-known cross-cut test. The notch frequencies of the scanned received signals were applied to obtain the NIR index. To avoid the interference of the thickness of coatings, the decimal part of NIR was further proposed to link with the bonding strength obtained by the cross-cut test. This index changes accordingly with aluminum- and magnesium-based coatings' bonding strength. The results prove that the proposed ultrasonic method based on the feedforward comb filter effect is high potential to assess the bonding strength of coatings nondestructively. [ABSTRACT FROM AUTHOR]

• A head-mounted transcranial ultrasound stimulation system for mice was built. • Ultrasound stimulation of DRN safely increased c-fos gene expression in the DRN. • Ultrasound stimulation of DRN increased 5-hydroxytryptamine levels in DRN. • Ultrasound stimulation of DRN promoted recovery from anesthesia and improved cognition in mice. • Antagonizing the 5-HT 2A receptor inhibited anesthesia recovery and cognition in mice after ultrasound stimulation of DRN. Delayed arousal and cognitive dysfunction are common, especially in older patients after general anesthesia (GA). Elevating central nervous system serotonin (5-HT) levels can promote recovery from GA and increase synaptic plasticity to improve cognition. Ultrasound neuromodulation has become a noninvasive physical intervention therapy with high spatial resolution and penetration depth, which can modulate neuronal excitability to treat psychiatric and neurodegenerative diseases. This study aims to use ultrasound to noninvasively modulate the brain 5-HT levels of mice to promote recovery from GA and improve cognition in mice. The dorsal raphe nucleus (DRN) of mice during GA was stimulated by the 1.1 MHz ultrasound with a negative pressure of 356 kPa, and the liquid chromatography coupled tandem mass spectrometry (LC-MS/MS) method was used to measure the DRN 5-HT concentrations. The mice's recovery time from GA was assessed, and the cognition was evaluated through spontaneous alternation Y-maze and novel object recognition (NOR) tests. After ultrasound stimulation, the mice's DRN 5-HT levels were significantly increased (control: 554.0 ± 103.2 ng/g, anesthesia + US: 664.2 ± 84.1 ng/g, *p = 0.0389); the GA recovery time (return of the righting reflex (RORR) emergence latency time) of mice was significantly reduced (anesthesia: 331.6 ± 70 s, anesthesia + US: 223.2 ± 67.7 s, *p = 0.0215); the spontaneous rotation behavior score of mice was significantly increased (anesthesia: 59.46 ± 5.26 %, anesthesia + US: 68.55 ± 5.24 %; *p = 0.0126); the recognition index was significantly increased (anesthesia: 55.02 ± 6.23 %, anesthesia + US: 78.52 ± 12.21 %; ***p = 0.0009). This study indicates that ultrasound stimulation of DRN increases serotonin levels, accelerates recovery from anesthesia, and improves cognition, which could be an important strategy for treating delayed arousal, postoperative delirium, or even lasting cognitive dysfunction after GA. [ABSTRACT FROM AUTHOR]