1. Evaluation of the protective effect of the intranasal vaccines adjuvanted with bacterium-like particles against intestinal infection.
- Author
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Tsujii A, Takahashi K, Harada H, Kawashima S, Oikawa H, Fukushima H, Hayakawa Y, Koizumi J, Inoue N, and Koshizuka T
- Subjects
- Animals, Mice, Antibodies, Bacterial immunology, Antibodies, Bacterial blood, Female, Adjuvants, Immunologic administration & dosage, Adhesins, Bacterial immunology, Adjuvants, Vaccine administration & dosage, Immunity, Mucosal, Immunoglobulin A, Secretory immunology, Immunoglobulin A immunology, Disease Models, Animal, Intestinal Diseases prevention & control, Intestinal Diseases immunology, Administration, Intranasal, Mice, Inbred C57BL, Citrobacter rodentium immunology, Citrobacter rodentium pathogenicity, Enterobacteriaceae Infections prevention & control, Enterobacteriaceae Infections immunology, Bacterial Vaccines immunology, Bacterial Vaccines administration & dosage
- Abstract
Mucosal vaccination presents a promising complement to parenteral vaccination. Bacterium-like particles (BLPs), peptidoglycan structures prepared from lactic acid bacteria, are explored as potential nasal vaccine adjuvants for respiratory infections. To date, studies on BLP-adjuvanted nasal vaccines against intestinal infections have remained limited. In this study, we demonstrated the efficacy of intranasal BLP-adjuvanted vaccination in controlling intestinal infections using the Citrobacter rodentium (C. rodentium) model in C57BL/6 mice. Intranasal vaccination of Intimin, an adhesin critical for intimate bacterial adhesion to colonic epithelial cells, combined with BLP (BLP+I) elicited robust Intimin-specific intestinal secretory IgA production, reduced bacterial load in feces and almost completely inhibited colonic hyperplasia, a characteristic symptom of C. rodentium infection in mice. Conversely, parenteral vaccination with Alhydrogel-adjuvanted Intimin failed to induce intestinal Intimin-specific IgA production, resulting in poor protection against C. rodentium infection. This underscores the pivotal role of mucosal IgA responses elicited by intranasal immunization in its protective efficacy. As this study did not delineate the precise protective mechanism conferred by BLP+I intranasal immunization against C. rodentium infection, further elucidation of the mechanisms underlying intranasal BLP+I immunization is required., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
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