1. The potential mechanism of Bursal-derived BPP-II on the antibody production and avian pre-B cell
- Author
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Feng, Xiuli, Cao, Ruibing, Zhou, Bin, Liu, Qingtao, Liu, Ke, Liu, Xiaodong, Zhang, Yuanpeng, Gu, Jinyan, Miao, Denian, and Chen, Puyan
- Subjects
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ANTIBODY formation , *B cells , *BURSA fabricii , *BIOACTIVE compounds , *BIRD diseases , *IMMUNE response , *GENE expression , *GENETIC transcription - Abstract
Abstract: The bursa of Fabricius is critical for the normal development of the B lymphocytes responsible for antibody production. However, the mechanism of the bursal-derived bioactive factor on B cell development is little reported. In this paper, chicks were immunized with BPP-II and AIV vaccine or AIV antigen, and antibody and IL-4 production were detected. The results showed that BPP-II played strongly inducing roles on the humoral immune responses. To investigate the gene expression at transcriptional level, avian pre-B lymphocyte DT40 cells were treated with BPP-II, and were analyzed with the gene microarray. The results proved that BPP-II treatment regulated 11 pathways, in which homologous recombination is a vital mechanism which is involved in antibody Ig gene conversion and diversification during B cell development. These results suggested Bursal-derived biological active factor BPP-II might be involved in the antibody production processes and B cell development, which is vital to the humoral central immune organ, the bursa of Fabricius. [Copyright &y& Elsevier]
- Published
- 2013
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