1. Nanoparticle formulation enhanced protective immunity provoked by PYGPI8p-transamidase related protein (PyTAM) DNA vaccine in Plasmodium yoelii malaria model
- Author
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Mohammed Nasir Shuaibu, Kenji Hirayama, Tomoaki Kurosaki, Hitoshi Sasaki, Tetsuo Yanagi, Juntra Karbwang, Mihoko Kikuchi, Yukinobu Kodama, Katsuyuki Yui, Gideon Kofi Helegbe, Nguyen Huy Tien, Akitoyo Ichinose, and Mahamoud Sama Cherif
- Subjects
T-Lymphocytes ,Protozoan Proteins ,Antibodies, Protozoan ,law.invention ,DNA vaccination ,Interferon-gamma ,Mice ,Immune system ,Plasmid ,Immunity ,law ,Malaria Vaccines ,Vaccines, DNA ,Animals ,Polyethyleneimine ,Cells, Cultured ,Immunity, Cellular ,General Veterinary ,General Immunology and Microbiology ,biology ,Public Health, Environmental and Occupational Health ,Dendritic Cells ,Plasmodium yoelii ,biology.organism_classification ,Interleukin-12 ,Virology ,Recombinant Proteins ,Immunity, Humoral ,Malaria ,Mice, Inbred C57BL ,Vaccination ,Infectious Diseases ,Polyglutamic Acid ,Humoral immunity ,Recombinant DNA ,Nanoparticles ,Molecular Medicine ,Female ,Spleen - Abstract
We have previously reported the new formulation of polyethylimine (PEI) with gamma polyglutamic acid (γ-PGA) nanoparticle (NP) to have provided Plasmodium yoelii merozoite surface protein-1 (PyMSP-1) plasmid DNA vaccine with enhanced protective cellular and humoral immunity in the lethal mouse malaria model. PyGPI8p-transamidase-related protein (PyTAM) was selected as a possible candidate vaccine antigen by using DNA vaccination screening from 29 GPI anchor and signal sequence motif positive genes picked up using web-based bioinformatics tools; though the observed protection was not complete. Here, we observed augmented protective effect of PyTAM DNA vaccine by using PEI and γ-PGA complex as delivery system. NP-coated PyTAM plasmid DNA immunized mice showed a significant survival rate from lethal P. yoelii challenge infection compared with naked PyTAM plasmid or with NP-coated empty plasmid DNA group. Antigen-specific IgG1 and IgG2b subclass antibody levels, proportion of CD4 and CD8T cells producing IFN-γ in the splenocytes and IL-4, IFN-γ, IL-12 and TNF-α levels in the sera and in the supernatants from ex vivo splenocytes culture were all enhanced by the NP-coated PyTAM DNA vaccine. These data indicates that NP augments PyTAM protective immune response, and this enhancement was associated with increased DC activation and concomitant IL-12 production.
- Published
- 2014
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