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7 results on '"Rossi-Bergmann, Bartira"'

7. Intranasal delivery of naked DNA encoding the LACK antigen leads to protective immunity against visceral leishmaniasis in mice

Author

Vicente Larraga, Ulisses Gazos Lopes, Wallace Pacienza Lima, Luiz Dione Barbosa de Melo, Bartira Rossi-Bergmann, Daniel Cláudio de Oliveira Gomes, Eduardo Fonseca Pinto, De Melo, Luiz Dione Barbosa [0000-0003-2445-9943], Lima, Wallace Pacienza [0000-0002-5489-2206], Larraga, Vicente [0000-0003-1260-7400], Lopes, Ulisses Gazos [0000-0002-0216-3482], Rossi-Bergmann, Bartira [0000-0002-3791-8996], De Melo, Luiz Dione Barbosa, Lima, Wallace Pacienza, Larraga, Vicente, Lopes, Ulisses Gazos, and Rossi-Bergmann, Bartira

Subjects
Protozoan Vaccines, Protozoan Proteins, Leishmaniasis, Cutaneous, Spleen, Antigens, Protozoan, Biology, Interferon-gamma, Mice, Cutaneous leishmaniasis, LACK, medicine, Animals, Leishmania infantum, Administration, Intranasal, Visceral leishmaniasis, Mice, Inbred BALB C, General Veterinary, General Immunology and Microbiology, Leishmania chagasi, Public Health, Environmental and Occupational Health, Leishmaniasis, DNA, DNA, Protozoan, medicine.disease, biology.organism_classification, Virology, Vaccination, Infectious Diseases, medicine.anatomical_structure, Treatment Outcome, Intranasal, Liver, Naked DNA, Immunology, Molecular Medicine, Leishmaniasis, Visceral, Interleukin-4, Vaccine, Plasmids
Abstract

5 p.-3 fig., We previously showed that intranasal (i.n.) vaccination with pCIneo plasmid encoding the leishmanial LACK gene (pCIneo-LACK) induces long-lasting protective immunity against cutaneous leishmaniasis in mice. In this work, we proposed to investigate whether the efficacy of i.n. pCIneo-LACK is extensive to visceral leishmaniasis. BALB/c mice received two i.n. doses of 30 μg pCIneo-LACK prior to intravenous (i.v.) infection with Leishmania chagasi. Vaccinated mice developed significantly lower parasite burden in the liver and spleen than control mice receiving empty pCIneo or saline. The spleen cells of vaccinated mice produced significantly increased IFN-γ and IL-4 concomitant with decreased IL-10 production during infection. Serum levels of specific IgG were elevated whereas TNF-α were decreased as compared with controls. These results show that the practical needle-free i.n. pCIneo-LACK vaccine displays potential broad-spectrum activity against leishmaniasis.

Published
2006

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