Search

Your search keyword '"Zhao, Qinjian"' showing total 15 results
Start Over Author "Zhao, Qinjian" Journal vaccine
15 results on '"Zhao, Qinjian"'

4. Characterization and immunogenicity of SARS-CoV-2 spike proteins with varied glycosylation

Author

Deng, Tingting, primary, Li, Tingting, additional, Chen, Gege, additional, Zhu, Yuhe, additional, Xu, Lang, additional, Lin, Yanlin, additional, Sun, Hui, additional, Zhang, Hui, additional, Fang, Qianjiao, additional, Hong, Junping, additional, Wu, Dinghui, additional, Gao, Shuangquan, additional, Li, Shaoyong, additional, Wang, Yingbin, additional, Zhang, Tianying, additional, Chen, Yixin, additional, Yuan, Quan, additional, Zheng, Qingbing, additional, Yu, Hai, additional, Zhao, Qinjian, additional, Zhang, Jun, additional, Li, Shaowei, additional, Xia, Ningshao, additional, and Gu, Ying, additional

Published
2022
Full Text
View/download PDF

8. Global vaccine action plan lessons learned I: Recommendations for the next decade

Author

MacDonald, Noni, primary, Mohsni, Ezzeddine, additional, Al-Mazrou, Yagob, additional, Kim Andrus, Jon, additional, Arora, Narendra, additional, Elden, Susan, additional, Madrid, Marie-Yvette, additional, Martin, Rebecca, additional, Mahmoud Mustafa, Amani, additional, Rees, Helen, additional, Salisbury, David, additional, Zhao, Qinjian, additional, Jones, Ian, additional, Steffen, Christoph A., additional, Hombach, Joachim, additional, O'Brien, Katherine L., additional, and Cravioto, Alejandro, additional

Published
2020
Full Text
View/download PDF

10. Epitope clustering analysis for vaccine-induced human antibodies in relationship to a panel of murine monoclonal antibodies against HPV16 viral capsid

Author

Zhang, Cai, primary, Huang, Xiaofen, additional, Chen, Siyi, additional, Li, Yike, additional, Li, Yufang, additional, Wang, Xin, additional, Tang, Jixian, additional, Xia, Lin, additional, Lin, Zhijie, additional, Luo, Wenxin, additional, Li, Tingdong, additional, Li, Shaowei, additional, Zhang, Jun, additional, Xia, Ningshao, additional, and Zhao, Qinjian, additional

Published
2018
Full Text
View/download PDF

11. Antigenic analysis of divergent genotypes human Enterovirus 71 viruses by a panel of neutralizing monoclonal antibodies: Current genotyping of EV71 does not reflect their antigenicity

Author

Chen, Yixin, primary, Li, Chuan, additional, He, Delei, additional, Cheng, Tong, additional, Ge, Shengxiang, additional, Shih, James Wai-Kuo, additional, Zhao, Qinjian, additional, Chen, Pei-Jer, additional, Zhang, Jun, additional, and Xia, Ningshao, additional

Published
2013
Full Text
View/download PDF

13. In-depth process understanding of RECOMBIVAX HB® maturation and potential epitope improvements with redox treatment: Multifaceted biochemical and immunochemical characterization

Author

Zhao, Qinjian, primary, Towne, Victoria, additional, Brown, Martha, additional, Wang, Yang, additional, Abraham, Dicky, additional, Oswald, C. Brent, additional, Gimenez, Juan A., additional, Washabaugh, Michael W., additional, Kennedy, Ronald, additional, and Sitrin, Robert D., additional

Published
2011
Full Text
View/download PDF

14. In-depth process understanding of RECOMBIVAX HB® maturation and potential epitope improvements with redox treatment: Multifaceted biochemical and immunochemical characterization

Author

Zhao, Qinjian, Towne, Victoria, Brown, Martha, Wang, Yang, Abraham, Dicky, Oswald, C. Brent, Gimenez, Juan A., Washabaugh, Michael W., Kennedy, Ronald, and Sitrin, Robert D.

Subjects
*OXIDATION-reduction reaction, *IMMUNOCHEMISTRY, *HEPATITIS B treatment, *MONOCLONAL antibodies, *CONFORMATIONAL analysis, *ENZYME-linked immunosorbent assay, *VIRAL vaccines
Abstract

Abstract: Recombinant Hepatitis B surface antigen virus-like particles (VLPs) produced in yeast undergo spontaneous maturation during the vaccine production process, and the biophysical characteristics of the particles with respect to maturation were described in Zhao et al. (2006) . Here we report additional biochemical and immunochemical characterization by various techniques, including the use of a panel of monoclonal antibodies (mAbs) that differ in their selectivity and conformation-sensitivity, for probing surface epitope structures. Crosslinking via interchain disulfide formation and binding of conformational specific antibodies in the mature particles were shown to be progressively enhanced. We show that redox-mediated VLP maturation is superior to heat-induced maturation in terms of generating VLPs which exhibit more complete crosslinking (>95%) and 2- to 3-fold higher antigenicity as defined by conformational antibodies. Therefore, the resulting VLPs from redox treatment resemble more closely their plasma-derived counterparts. The value of using multiple mAbs for probing surface epitopes was clearly demonstrated as different mAbs showed different degrees of sensitivity to the structural changes during HBsAg VLP maturation. The rapid, label-free technology of surface plasmon resonance performed at a single antigen concentration was shown to correlate well with a sandwich ELISA using parallel line analysis, currently implemented for product release and stability testing of RECOMBIVAX HB®. Surface plasmon resonance offers both convenience and flexibility; multiple mAbs can be tested one at a time in the same set of experiments, providing a means to assess changes to individual epitopes. Taken together, these quantitative analytical tools enable more rapid, in-depth, and comprehensive process monitoring, process optimization, and assessment of product consistency and stability. [Copyright &y& Elsevier]

Published
2011
Full Text
View/download PDF

15. Robust manufacturing and comprehensive characterization of recombinant hepatitis E virus-like particles in Hecolin®.

Author

Zhang, Xiao, Wei, Minxi, Pan, Huirong, Lin, Zhijie, Wang, Kaihang, Weng, Zusen, Zhu, Yibin, Xin, Lu, Zhang, Jun, Li, Shaowei, Xia, Ningshao, and Zhao, Qinjian

Subjects
*HEPATITIS E virus, *VIRUS-like particles, *RECOMBINANT microorganisms, *RECOMBINANT proteins, *CAPSIDS, *VIRAL proteins
Abstract

Highlights: [•] Recombinant capsid protein of hepatitis E virus as vaccine antigen. [•] Multiple epitope characterization using monoclonal antibodies. [•] Successful scale up and robust manufacturing of vaccine antigen. [•] Product consistency demonstrated with orthogonal analytical methods. [•] In-depth characterization of recombinant virus-like particles. [Copyright &y& Elsevier]

Published
2014
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results