3 results on '"Lima Barbero, José Francisco"'
Search Results
2. A vaccinology Approach to the Identification and Characterization of Dermanyssus Gallinae Candidate Protective Antigens for the Control of Poultry Red Mite Infestations
- Author
-
Kathryn Bartley, Francisco Manuel Mata-Lorenzo, Margarita Villar, Daniel R. G. Price, Francesca Nunn, Olivier Sparagano, Lourdes Mateos-Hernández, Christina Strube, José Francisco Lima-Barbero, Robert D. Finn, Alasdair J. Nisbet, José de la Fuente, Roxana Triguero-Ocaña, Marinela Contreras, Mariana Boadella, Ursula Höfle, European Commission, Universidad de Castilla La Mancha, Ministerio de Ciencia, Innovación y Universidades (España), Lima-Barbero, José Francisco [0000-0002-2694-0215], Mateos-Hernández, Lourdes [0000-0001-9566-7710], Höfle, Ursula [0000-0002-6868-079X], Fuente, José de la [0000-0001-7383-9649], Villar, Margarita [0000-0003-4172-9079], Lima-Barbero, José Francisco, Mateos-Hernández, Lourdes, Höfle, Ursula, Fuente, José de la, Villar, Margarita, SaBio, Instituto de Investigación en Recursos Cinegéticos (IREC), Sabiotec, Polivalente UCLM, Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Department of Infectious Diseases and Public Health, City University of Hong Kong [Hong Kong] (CUHK), Department of Applied Sciences, Faculty of Health & Life Sciences, University of Northumbria at Newcastle [United Kingdom]-University of Northumbria at Newcastle [United Kingdom], St George’s International School of Medicine, Keith B. Taylor Global Scholars Program, Institute for Parasitology, Centre for Infection Medicine, University of Veterinary Medicine Hannover-University of Veterinary Medicine Hannover, Moredun Research Institute [Penicuik, UK] (MRI), Department of Veterinary Pathobiology, Center of Veterinary Health Sciences, Oklahoma State University [Stillwater] (OSU)-Oklahoma State University [Stillwater] (OSU), Biochemistry Section, Faculty of Science and Chemical Technologies, Regional Centre for Biomedical Research (CRIB), Universidad de Castilla-La Mancha = University of Castilla-La Mancha (UCLM)-Universidad de Castilla-La Mancha = University of Castilla-La Mancha (UCLM)-Regional Centre for Biomedical Research (CRIB), Universidad de Castilla-La Mancha = University of Castilla-La Mancha (UCLM)-Universidad de Castilla-La Mancha = University of Castilla-La Mancha (UCLM), Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire d'Alfort (ENVA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Oklahoma State University [Stillwater]-Oklahoma State University [Stillwater], University of Castilla-La Mancha (UCLM)-University of Castilla-La Mancha (UCLM)-Regional Centre for Biomedical Research (CRIB), and University of Castilla-La Mancha (UCLM)-University of Castilla-La Mancha (UCLM)
- Subjects
0301 basic medicine ,Proteomics ,food.ingredient ,Dermanyssus gallinae ,Poultry red mite ,viruses ,030231 tropical medicine ,Immunology ,lcsh:Medicine ,Article ,03 medical and health sciences ,Protective antigens ,0302 clinical medicine ,food ,proteomics ,Antigen ,vaccine ,Drug Discovery ,parasitic diseases ,Mite ,Pharmacology (medical) ,Dermanyssus ,Nymph ,poultry red mite ,Pharmacology ,Mite Infestations ,biology ,fungi ,lcsh:R ,dermanyssus ,biology.organism_classification ,Virology ,3. Good health ,Vaccinology ,030104 developmental biology ,Infectious Diseases ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,Rhipicephalus microplus ,vaccinology ,PEST analysis ,[SDV.IMM.VAC]Life Sciences [q-bio]/Immunology/Vaccinology ,Vaccine ,control ,protective antigens - Abstract
The poultry red mite (PRM), Dermanyssus gallinae, is a hematophagous ectoparasite considered as the major pest in the egg-laying industry. Its pesticide-based control is only partially successful and requires the development of new control interventions such as vaccines. In this study, we follow a vaccinology approach to identify PRM candidate protective antigens. Based on proteomic data from fed and unfed nymph and adult mites, we selected a novel PRM protein, calumenin (Deg-CALU), which is tested as a vaccine candidate on an on-hen trial. Rhipicephalus microplus Subolesin (Rhm-SUB) was chosen as a positive control. Deg-CALU and Rhm-SUB reduced the mite oviposition by 35 and 44%, respectively. These results support Deg-CALU and Rhm-SUB as candidate protective antigens for the PRM control., This work was partially supported by the European Cooperation in Science and Technology (COST), Improving current understanding and research for sustainable control of the poultry red mite Dermanyssus gallinae – COREMI project (Action FA1404). JFLB was supported by Ministerio de Ciencia, Innovación y Universidades (Spain), Doctorado Industrial contract (DI-14-06917) and Sabiotec SA. MV was supported by the University of Castilla- La Mancha (Spain).
- Published
- 2019
3. Reduction in Oviposition of Poultry Red Mite (Dermanyssus gallinae) in Hens Vaccinated with Recombinant Akirin
- Author
-
Daniel R. G. Price, José de la Fuente, Ursula Höfle, Francesca Nunn, Alasdair J. Nisbet, Eduardo Prado, Marta Sanchez-Sanchez, Marinela Contreras, José Francisco Lima-Barbero, Margarita Villar, Kathryn Bartley, Ministerio de Ciencia, Innovación y Universidades (España), European Commission, Universidad de Castilla La Mancha, Lima-Barbero, José Francisco [0000-0002-2694-0215], Contreras, Marinela [0000-0002-1505-5624], Höfle, Ursula [0000-0002-6868-079X], Villar, Margarita [0000-0003-4172-9079], Fuente, José de la [0000-0001-7383-9649], Lima-Barbero, José Francisco, Contreras, Marinela, Höfle, Ursula, Villar, Margarita, and Fuente, José de la
- Subjects
0301 basic medicine ,Veterinary medicine ,food.ingredient ,Poultry red mite ,Dermanyssus gallinae ,viruses ,030231 tropical medicine ,Immunology ,lcsh:Medicine ,Tick ,03 medical and health sciences ,0302 clinical medicine ,food ,vaccine ,hemic and lymphatic diseases ,parasitic diseases ,Drug Discovery ,Mite ,Pharmacology (medical) ,Dermanyssus ,Subolesin ,poultry red mite ,Pharmacology ,biology ,Acaricide ,business.industry ,lcsh:R ,Poultry farming ,biology.organism_classification ,tick ,Vaccination ,030104 developmental biology ,Infectious Diseases ,Akirin ,biology.protein ,Antibody ,business ,Vaccine ,control - Abstract
The poultry red mite (PRM), Dermanyssus gallinae, is a hematophagous ectoparasite of birds with worldwide distribution that causes economic losses in the egg-production sector of the poultry industry. Traditional control methods, mainly based on acaricides, have been only partially successful, and new vaccine-based interventions are required for the control of PRM. Vaccination with insect Akirin (AKR) and its homolog in ticks, Subolesin (SUB), have shown protective efficacy for the control of ectoparasite infestations and pathogen infection/transmission. The aim of this study was the identification of the akr gene from D. gallinae (Deg-akr), the production of the recombinant Deg-AKR protein, and evaluation of its efficacy as a vaccine candidate for the control of PRM. The anti-Deg-AKR serum IgY antibodies in hen sera and egg yolk were higher in vaccinated than control animals throughout the experiment. The results demonstrated the efficacy of the vaccination with Deg-AKR for the control of PRM by reducing mite oviposition by 42% following feeding on vaccinated hens. A negative correlation between the levels of serum anti-Deg-AKR IgY and mite oviposition was obtained. These results support Deg-AKR as a candidate protective antigen for the control of PRM population growth., This work was partially supported by COST Action FA 1404 (COREMI) (https://www.coremi.eu/home.html). JFLB was supported by Doctorado Industrial contract DI-14-06917 from Ministerio de Ciencia, Innovación y Universidades (Spain) and Sabiotec S.A. MV was supported by the Research Plan of the University of Castilla-La Mancha (Spain).
- Published
- 2019
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.