1. Molecular detection of Orthoflavivirus in wild birds from Yucatan, Mexico.
- Author
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Santiago-Bautista, Erik Gustavo, Hernández-Villegas, Erika Nayelli, Jiménez-Rico, Marco Antonio, García-Hernández, Montserrat Elemi, Sarmiento-Silva, Rosa Elena, Gerardo, Suzán, and Vigueras-Galván, Ana Laura more...
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FLAVIVIRUSES , *PASSERIFORMES , *ZOONOSES - Abstract
Orthoflaviviruses annually infect over 400 million people worldwide. These arboviruses are transmitted by mosquitoes of the Aedes, Culex genera,, some bird species are recognized as reservoirs, mostly of the Passeriformes order. Given Yucatan’s bird richness, it is necessary to emphasize the need to monitor Orthoflavivirus circulation in this taxon from the region. The main aim of this study is to identify Orthoflavivirus presence in wild birds inhabiting a gradient of different land use (Conserved, Diversified, Rural, Urban) in Yucatan, Mexico. The captured birds were classified by species, a cloacal swab was collected. Samples of the species considered as reservoirs or possible reservoirs of Orthoflavivirus were selected, analyzed using a nested RT-PCR for the amplification of a fragment of the NS5 gene, positive results were confirmed by Sanger sequencing. A total of 423 birds were sampled which 218 samples are from reservoirs, possible reservoirs, being the Rural habitat with the greatest abundance of these (88 of 233 birds). So far,134 samples have been analyzed; with two positive results obtained, but continuous monitoring of bird communities is necessary to assess the relationship between land-use changes, the Orthoflavivirus cycle in Yucatan. [ABSTRACT FROM AUTHOR] more...
- Published
- 2024
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