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Miconazole-tolerant strains of Malassezia pachydermatis generated by culture in medium containing miconazole I Malassezia i species, which can be lipophilic or lipid-dependent, are part of the resident skin flora of animals, and also are associated with skin diseases in dogs and cats.[[1]] Canine I Malassezia i dermatitis often is treated by topical therapies, such as shampoos containing 2% miconazole (MCZ) and 2% chlorhexidine, or other topical products containing MCZ.[[1]] Miconazole is a member of the azole family of antifungal agents and works by decreasing the ability of fungi to produce ergosterol, an important part of its cell membrane. The antifungal activities of MCZ and tacrolimus, alone and in combination, were tested in the MCZ-tolerant strains cultured in SDB supplemented with 1% Tween 80 after four days of incubation at 32°C. The MCZ-tolerant strains exhibited MICs of 40 mg/L for MCZ alone and 32 mg/L for tacrolimus alone; the MCZ MIC to 40 mg/L addition of tacrolimus, yielding an MCZ FICI value of 2 (calculation; 40/ 40 + 32/ 32 = 2). [Extracted from the article]

We have reported on a terbinafine (TERB)-resistant I Microsporum canis i strain (designated 47C) from a cat with dermatophytosis in China that had a minimum inhibitory concentration (MIC) of >32 mg/L.[1] Ravuconazole (RVCZ) is a broadspectrum antifungal azole drug which exhibits excellent activity against dermatophytes.[2] We report herein the I in vitro i susceptibility of clinical isolates of dermatophytes and the TERB-resistant strain of I M. canis i to RVCZ, itraconazole (ITZ) and TERB. Minimal inhibitory concentration (MIC) of antifungal drugs against the tested dermatophyte species. [Extracted from the article]

One of the mechanisms of eosinophil infiltration is its induction by chemoattractants such as regulated upon activation, normal T-expressed and secreted (RANTES) which is a cysteine–cysteine chemokine that mediates chemotaxis and activation of eosinophils in humans and mice. Skin lesions of feline eosinophilic plaque are characterized by a predominant infiltration of eosinophils. The mechanism(s) of eosinophilic infiltration in the skin and/or mucosa of cats is unknown. It is possible that RANTES is involved. To investigate the presence of RANTES in the skin of cats with eosinophilic plaques and nonaffected skin, we cloned and sequenced the full-length feline RANTES cDNA gene, in order to determine whether it is present in the skin of cats with eosinophilic plaques and/or if it is present in normal adjacent skin. We were able to document the the expression of RANTES mRNAs in skin with feline eosinophilic plaque as well as in normal cat skin. The full-length cDNA sequence of the RANTES gene (742 bp) contained a single open reading frame of 276 bp encoding a protein of 92 amino acids. The amino acid sequence of feline RANTES shared 67 and 74% sequence identity with that of bovine and mouse RANTES genes, respectively. RT–PCR analysis on RANTES mRNA in the skin of cats with eosinophilic plaque revealed that its expression was higher in the eosinophilic plaque skin lesions than in the normal skin. The result suggested that RANTES might play a role to induce eosinophil infiltration in feline eosinophilic plaque lesions. [ABSTRACT FROM AUTHOR]