1. Molecular Profiling Reveals Prognostically Significant Subtypes of Canine Lymphoma
- Author
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Kerstin Lindblad-Toh, Brian D. Husbands, Timothy O'Brien, William C. Kisseberth, Matthew Breen, Lawrence Hunter, Tzu L. Phang, Daisuke Ito, Jaime F. Modiano, Kristine Burgess, Michael S. Henson, Victor E. Valli, Aaron L. Sarver, Aric M. Frantz, Antonella Borgatti, Anis Karimpour-Fard, and Milcah C. Scott
- Subjects
Male ,Pathology ,medicine.medical_specialty ,Lymphoma, B-Cell ,Genome-wide association study ,Biology ,Lymphoma, T-Cell ,Disease-Free Survival ,Article ,Immunophenotyping ,Cohort Studies ,Dogs ,immune system diseases ,hemic and lymphatic diseases ,Gene expression ,Biomarkers, Tumor ,medicine ,Animals ,Dog Diseases ,RNA, Neoplasm ,Gene ,Oligonucleotide Array Sequence Analysis ,Canine Lymphoma ,General Veterinary ,Gene Expression Profiling ,Not Otherwise Specified ,Computational Biology ,Prognosis ,medicine.disease ,Lymphoma ,Gene Expression Regulation, Neoplastic ,Gene expression profiling ,Female ,Genome-Wide Association Study - Abstract
We performed genomewide gene expression analysis of 35 samples representing 6 common histologic subtypes of canine lymphoma and bioinformatics analyses to define their molecular characteristics. Three major groups were defined on the basis of gene expression profiles: (1) low-grade T-cell lymphoma, composed entirely by T-zone lymphoma; (2) high-grade T-cell lymphoma, consisting of lymphoblastic T-cell lymphoma and peripheral T-cell lymphoma not otherwise specified; and (3) B-cell lymphoma, consisting of marginal B-cell lymphoma, diffuse large B-cell lymphoma, and Burkitt lymphoma. Interspecies comparative analyses of gene expression profiles also showed that marginal B-cell lymphoma and diffuse large B-cell lymphoma in dogs and humans might represent a continuum of disease with similar drivers. The classification of these diverse tumors into 3 subgroups was prognostically significant, as the groups were directly correlated with event-free survival. Finally, we developed a benchtop diagnostic test based on expression of 4 genes that can robustly classify canine lymphomas into one of these 3 subgroups, enabling a direct clinical application for our results.
- Published
- 2012