Search

Present investigation was undertaken on three buffalo bulls (Murrah) to study the leakage of phosphatases during cryopreservation. A total of 18 collections from three bulls (six collections per bull) were utilized for the study. After initial evaluation, each semen sample was diluted in two different extenders viz. Tris egg yolk extender which acted as control and Soya-milk extender as experimental. At the equilibration and post-thaw stages leakage of phosphatases viz. alkaline phosphatase (AKP) and acid phosphatase (ACP) were studied. There was a significant difference in the levels of AKP and ACP at post thaw stages in between the extenders. The leakage of phosphatases was found to increase from pre-freeze to post-freeze stage in both the extenders with better results in Tris egg yolk. [ABSTRACT FROM AUTHOR]

A study was undertaken to assess the efficacy of cysteine hydrochloride concentrations on post-thaw progressive motility, live sperm, acrosome integrity and sperm abnormalities following freezing and thawing in Jersey bulls semen. Semen was collected from 3 purebred Jersey bulls twice a week and 18 collections (6 collections per bull) were utilized in this study. After collection and primary evaluation, samples were divided into four fractions and were diluted in egg yolk tris glycerol extender with addition of cysteine @ 10, 12 or 15 mM (CYS-10, CYS-12 and CYS-15, respectively) and without cysteine (EYTG-C) which acted as control. Filling and sealing of diluted semen samples in PVA straws, equilibration at 4°C for 4 h, freezing in programmable bio-freezer and plunging of straws into liquid nitrogen were followed as per standard procedures. After thawing at 37°C for 30 sec, semen characteristics such as progressive motility, live sperm percentage and acrosome integrity were significantly higher whereas sperm abnormalities were significantly lower in samples containing cysteine @ 10 mM over the control and other dilutors. The results revealed that addition of cysteine @ 10 mM as an additive to semen in tris egg yolk extender, significantly improves the post-thaw semen characteristics, whereas adverse effect of cysteine was evident in its higher concentrations i.e. 12 mM and 15 mM. [ABSTRACT FROM AUTHOR]