1. Double-labelling immunohistochemical study of megakaryocytes in African swine fever
- Author
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M.J. Bautista, Francisco Rodríguez, José Andrés Moreno Pérez, P. J. Wilkinson, M. A. Sierra, and J. Martín de las Mulas
- Subjects
Male ,General Veterinary ,Swine ,Inoculation ,Karyorrhexis ,Virulence ,General Medicine ,Biology ,biology.organism_classification ,African Swine Fever Virus ,Immunohistochemistry ,Virology ,African swine fever virus ,Virus ,medicine.anatomical_structure ,Bone Marrow ,medicine ,Animals ,Female ,Bone marrow ,African Swine Fever ,Megakaryocytes ,Pyknosis - Abstract
Bone marrow samples from pigs infected with the highly virulent Malawi983 or moderately virulent Dominican Republic (DR978) isolates of African swine fever virus were studied by means of a double labelling immunohistochemical technique which stained the major structural protein VP73 of the virus and megakaryocytes simultaneously. In pigs infected with the highly virulent Malawi983 isolate, 2.2 per cent of megakaryocytes were VP73+ five days after inoculation, and at six and seven days 2.5 and 9.5 per cent of megakaryocytes were VP73+. Some infected and uninfected megakaryocytes showed pyknosis and karyorrhexis, particularly at seven days after inoculation. However, in comparison with uninfected pigs, the number of megakaryocytes decreased only at seven days after inoculation. In pigs infected with the moderately virulent DR978 isolate, only 0.2 per cent of megakaryocytes were VP73+ at eight days after inoculation. However, at eight, nine and 10 days after inoculation the total number of megakaryocytes was significantly lower (P
- Published
- 1997
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