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Simple Summary: The study focuses on the genetic characteristics of the lumpy skin disease virus (LSDV) from non-vaccinated beef cattle with LSD symptoms that lived in areas that had received live-attenuated vaccines in Lopburi, Thailand. Four skin positive samples through real-time PCR underwent virus isolation on cell cultures, followed by whole genome sequencing. The phylogenetic and mutation analyses were used to compare sequences between four LSDV samples from Lopburi with the LSDV/Thailand/YST/2021 strain (original strain in Thailand). In conclusion, four LSDVs in Lopburi during 2021–2022 were recombinant strains, clade 2.5, same as the LSDV/Thailand/YST/2021 strain. However, the viruses had mutations of open reading frame (ORF) 023 with significant amino acid deletion in two samples from Chai Badan district and of the untranslated region between ORF022 and ORF023 in two samples from Lam Sothi district. Lumpy skin disease (LSD) is a viral infection that impacts the cattle industry. The most efficient approach to prevent disease involves the utilization of live-attenuated LSD vaccines (LAVs), which stands out as the most successful method. However, LAVs might be subjected to changes to their genomes during replication that increase viral infectivity or virulence. The objective of this study was to monitor alterations in the genetic characteristics of the lumpy skin disease virus (LSDV) in beef cattle following the administration of LAVs in Lopburi Province of Central Thailand. A total of four skin samples from LSD cases were collected from non-vaccinated animals that exhibited LSD clinical symptoms from two distinct districts, spanning three subdistricts within the region. The samples of cattle were analyzed using real-time PCR targeting the LSDV074 p32 gene, the virus was isolated, and the entire genome sequences were evaluated through a single nucleotide polymorphisms (SNPs) analysis, and phylogenetic trees were assembled. The investigations revealed that LSDVs from two isolates from Chai Badan district exhibited significant mutations in the open reading frame (ORF) 023 putative protein, while another two isolates from Lam Sonthi district had a change in the untranslated region (UTR). For a result, the most proficient disease diagnosis and control should be evaluated on viral genetics on a regular basis. [ABSTRACT FROM AUTHOR]

Simple Summary: Forty SARS-CoV-2-positive swab samples from 40 animals owned by confirmed SARS-CoV-2-infected owners in Korea in 2021 were analyzed using next-generation sequencing (NGS) to identify the genomic lineage of the viruses. Among the 40 animal samples, eight Pango lineages (B.1.1.7) (Alpha variant), B.1.429 (Epsilon variant), B.1.470, B.1.497, B.1.619.1, B.1.620, AY.69 (Delta variant), and AY.122.5 (Delta variant) were identified. This study provides the first reported cases of six lineages (B.1.470, B.1.497, B.1.620, B.1.619.1, AY.69 (Delta variant), and AY.122.5 (Delta variant)) in cats and dogs. Our results emphasize the importance of monitoring SARS-CoV-2 in pets because they are dynamic hosts of variant Pango lineages of SARS-CoV-2. SARS-CoV-2 infections have caused unprecedented damage worldwide by affecting humans and various animals. The first reported animal infection was observed in a pet dog in Hong Kong in March 2020. 36 countries reported 692 SARS-CoV-2 infections in 25 different animal species by 31 August 2022. Most outbreaks were caused by contact with SARS-CoV-2 infected humans. In South Korea, the first SARS-CoV-2 infection in an animal was reported in a cat in February 2021. As of 31 December 2021, 74 dogs and 42 cats have been confirmed to have SARS-CoV-2 in South Korea. Here, we identified various SARS-CoV-2 genomic lineages in SARS-CoV-2 confirmed cats and dogs. Among the 40 animal samples sequenced for lineage identification, a total of eight Pango lineages (B.1.1.7 (Alpha variant), B.1.429 (Epsilon variant), B.1.470, B.1.497, B.1.619.1, B.1.620, AY.69 (Delta variant), and AY.122.5 (Delta variant)) were identified. The dominant lineages were AY.69 (Delta variant; 37.5%), B.1.497 (35.0%), and B.1.619.1 (12.5%). This study provides the first reported cases of six lineages (B.1.470, B.1.497, B.1.620, B.1.619.1, AY.69 (Delta variant)), and AY.122.5 (Delta variant) in cats and dogs. Our results emphasize the importance of monitoring SARS-CoV-2 in pets because they are dynamic hosts of variant Pango lineages of SARS-CoV-2. [ABSTRACT FROM AUTHOR]