1. Induction of Immune Responses by Recombinant PH-1 Domain of Infectious Laryngotracheitis Virus Glycoprotein B in Chickens
- Author
-
Faramarzi Samireh, Shahsavandi Shahla, and Ebrahimi Mohammad Majid
- Subjects
Immunology ,Biology ,Antibodies, Viral ,Vaccines, Attenuated ,law.invention ,Immune system ,Herpesvirus 1, Gallid ,Viral Envelope Proteins ,law ,Virology ,medicine ,Animals ,Glycoprotein B ,Adverse effect ,Poultry Diseases ,Infectious laryngotracheitis virus ,Attenuated vaccine ,Respiratory disease ,Immunity ,Viral Vaccines ,Herpesviridae Infections ,medicine.disease ,Ectodomain ,Recombinant DNA ,Molecular Medicine ,Chickens - Abstract
Infectious laryngotracheitis virus (ILTV) is a cause of main respiratory disease of chickens controlled through live attenuated vaccines. To reduce the risk of adverse effects associated with live vaccines, a recombinant vaccine expressing PH-1 domain of viral glycoprotein B was constructed using the pET expression system under isopropylthiogalactoside (IPTG) induction. The potential immunogenicity of recombinant PH-1 (rPH-1) was evaluated in chickens. Eight-week-old specific-pathogen-free chickens were intramuscularly administered two doses of rPH-1, 25 and 50 μg, alone or with a combination of ISA70 adjuvant. The humoral immune responses were determined up to 3 months postvaccination at 2 weeks apart. The T cell proliferation response was determined on day 28 after primary immunization. The vaccinated birds with rPH-1/ISA70 developed higher and constant-specific anti-ILTV enzyme-linked immunosorbent assay (ELISA) antibodies than in those vaccinated with rPH-1 alone. Coinjection of rPH-1 and adjuvant significantly (
- Published
- 2021