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Start Over Author "Shahsavandi, Shahla" Journal viral immunology
4 results on '"Shahsavandi, Shahla"'

2. Induction of Immune Responses by Recombinant PH-1 Domain of Infectious Laryngotracheitis Virus Glycoprotein B in Chickens

Author

Faramarzi Samireh, Shahsavandi Shahla, and Ebrahimi Mohammad Majid

Subjects
Immunology, Biology, Antibodies, Viral, Vaccines, Attenuated, law.invention, Immune system, Herpesvirus 1, Gallid, Viral Envelope Proteins, law, Virology, medicine, Animals, Glycoprotein B, Adverse effect, Poultry Diseases, Infectious laryngotracheitis virus, Attenuated vaccine, Respiratory disease, Immunity, Viral Vaccines, Herpesviridae Infections, medicine.disease, Ectodomain, Recombinant DNA, Molecular Medicine, Chickens
Abstract

Infectious laryngotracheitis virus (ILTV) is a cause of main respiratory disease of chickens controlled through live attenuated vaccines. To reduce the risk of adverse effects associated with live vaccines, a recombinant vaccine expressing PH-1 domain of viral glycoprotein B was constructed using the pET expression system under isopropylthiogalactoside (IPTG) induction. The potential immunogenicity of recombinant PH-1 (rPH-1) was evaluated in chickens. Eight-week-old specific-pathogen-free chickens were intramuscularly administered two doses of rPH-1, 25 and 50 μg, alone or with a combination of ISA70 adjuvant. The humoral immune responses were determined up to 3 months postvaccination at 2 weeks apart. The T cell proliferation response was determined on day 28 after primary immunization. The vaccinated birds with rPH-1/ISA70 developed higher and constant-specific anti-ILTV enzyme-linked immunosorbent assay (ELISA) antibodies than in those vaccinated with rPH-1 alone. Coinjection of rPH-1 and adjuvant significantly (

Published
2021

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