1. A novel human monoclonal antibody potently neutralizes human adenovirus serotype 7 by primarily targeting the adenovirus hexon protein.
- Author
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Lu, Jiansheng, Wang, Rong, Huang, Ying, Yu, Yunzhou, Zhou, Xiaowei, Huang, Peitang, and Yang, Zhixin
- Subjects
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ADENOVIRUSES , *MONOCLONAL antibodies , *RESPIRATORY diseases , *RECOMBINANT proteins , *PLASMA cells , *BLOOD cells , *CARRIER proteins - Abstract
Human adenovirus serotype 7 (HAdV-7), belonging to species B, has caused severe lower respiratory tract diseases and even deaths recently. However, no adenovirus vaccine or therapeutic is available thus far. In this study, a HAdV-7-specific human monoclonal antibody (HMAb), 3-3E, isolated from single plasma cells obtained from the peripheral blood mononuclear cells of HAdV-7-infected patients showed potent HAdV-7 neutralization activity. The results showed HMAb 3-3E only binds to the hexon protein of intact HAdV-7 or the recombinant hexon protein and it does not bind to other intact virion particles. This could mean the antibody recognizes a conformational epitope of the hexon protein. Further, HMAb 3-3E potently neutralized HAdV-7 in vitro at low concentrations. In vivo studies showed HMAb 3-3E protected from HAdV-7 infection in a murine model. Therefore, HMAb 3-3E is promising as a safe and effective prophylactic and therapeutic treatment for HAdV-7 infection. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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