1. Establishing an accurate and sensitive in vitro drug screening system for human adenovirus infection with human corneal cells.
- Author
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Sasano, Mina, Hayashi, Hironori, Kawaji, Kumi, Usui, Emiko, and Kodama, Eiich N.
- Subjects
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ADENOVIRUSES , *MEDICAL screening , *EYE infections , *COMMUNICABLE diseases , *CORNEA , *HUMAN adenoviruses - Abstract
Epidemic keratoconjunctivitis (EKC) is a hazardous and highly contagious disease, with the potential to cause epidemic outbreaks in hospitals and other community settings. There are currently no approved drugs for human adenovirus (HAdV), the causative agent of EKC. To establish a novel drug screening system for ocular HAdV infections, we employed CRL11516, a non-cancerous but immortalized human corneal epithelial cell line. Brincidoforvir and 3′-deoxy-3′-fluorothymidine inhibit replication of HAdV species C type 1 (C1), C2, E4, and C6 to the same extent. This alternative assay system may allow for the evaluation of anti-HAdV activity and cell cytotoxicity of compounds within 2 days and without the need of the rabbit eye infection model. • A human epithelial cell line, CRL-11516, support infection of human adenoviruses. • Potency of anti-adenoviral agents can be evaluated within 2 days. • The assay system is a useful model of adenoviral keratoconjunctivitis in vitro. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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