1. Regional mutations in CHIKV-ECSA genomes and detection of other viruses in the serum of acute febrile patients by a metagenomic approach in Mato Grosso, Central-Western Brazil, 2018.
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Mendes dos Santos, Marcelo Adriano, Dias, Lucas Silva, Ramirez Pavon, Janeth Aracely, Viniski, Ana Elisa, Campos Souza, Claudio Luís, Pepato, Marco Andrey, Correa de Azevedo, Vergínia, Teixeira Nunes, Marcio Roberto, and Slhessarenko, Renata Dezengrini
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METAGENOMICS , *SHOTGUN sequencing , *TORQUE teno virus , *GENOMES , *VIRAL genomes , *ARBOVIRUS diseases , *PARVOVIRUS B19 - Abstract
Mato Grosso (MT) State is part of central western Brazil and has a tropical permissive environment that favors arbovirus outbreaks. A metagenomic approach was used to identify viral genomes in seven pools of serum from patients (n=65) with acute febrile disease. Seven chikungunya virus (CHIKV) genomes were determined, showing four amino acid changes found only in CHIKV genomes obtained in MT since 2018: nsP2:T31I, nsP3: A388V, E3:T201I and E3:H57R, in addition to other mutations in E1, nsP2 and nsP4. Six parvovirus B19 (B19V) genotype I genomes (4771–5131 nt) showed four aa alterations (NS1:N473D, R579Q; VP1:I716T; and 11 kDa:V44A) compared to most similar B19V from the USA. Coinfection between CHIKV and B19V was evidenced in 22/65 (33.8%) patients by RT‒PCR and PCR, respectively. Other viruses found in these pools include human pegivirus C , torque teno virus 3, an unclassified TTV and torque teno mini virus. Metagenomics represents a useful approach to detect viruses in the serum of acute febrile patients suspected of arbovirus disease. Metagenomics represent a useful tool to identify viral genomes present in a sample. CHIKV ECSA genomes present regional aa changes: nsP2:T31I, nsP3: A388V, E3:T201I and E3:H57R. B19V genotype 1 was prevalent in patients with arbovirus-suspected disease. This is the first description of Human Pegivirus C in patients from Mato Grosso. Three anelloviruses were detected in the serum of patients, mainly in children. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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