1. A novel peptide-based vaccine candidate with protective efficacy against influenza A in a mouse model
- Author
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Maja A. Sommerfelt, Birger Sørensen, Tjarko Meijerhof, José Herrera-Rodriguez, Anke Huckriede, Mats Ökvist, Arnt-Ove Hovden, Hubert G. M. Niesters, Grete Stjernholm, Microbes in Health and Disease (MHD), and Translational Immunology Groningen (TRIGR)
- Subjects
0301 basic medicine ,Influenza vaccine ,Epitopes, T-Lymphocyte ,Hemagglutinin (influenza) ,Mice, Transgenic ,Human leukocyte antigen ,Cell mediated immunity ,IMMUNITY ,Biology ,Epitope ,Mice ,Random Allocation ,03 medical and health sciences ,Immune system ,T-CELL EPITOPES ,Immunity ,Virology ,HLA-A2 Antigen ,Influenza, Human ,Animals ,Humans ,Immunity, Cellular ,Influenza ,Immunity, Humoral ,Mice, Inbred C57BL ,Disease Models, Animal ,Humoral immunity ,Universal vaccine ,030104 developmental biology ,Influenza A virus ,Influenza Vaccines ,SAFETY ,biology.protein ,VIRUS ,Female ,Immunization ,TRIAL ,Antibody ,Peptides ,RESPONSES - Abstract
Current influenza vaccines mainly induce antibody responses to the variable hemagglutinin proteins of the virus strains included in the vaccine. Instead, a broadly protective influenza vaccine should aim at inducing antibody and/or cell -mediated immunity against conserved viral proteins. Vacc-FLU is a peptide based vaccine combining conserved B and T cell epitopes. Peptide selection was done using a proprietary peptide design platform technology focusing on responses to human leukocyte antigen (HLA)-restricted epitopes. Immunization of wild -type mice and mice transgenic for HLA-A2.1 with the peptide mix successfully induced both humoral and cell mediated immune responses. Partial protection from severe weight loss upon challenge was observed in both mouse strains but was stronger and observed at lower vaccine doses in transgenic mice. Our results show that the Vacc-FLU peptide mix is capable of inducing IFNy-producing T cells and antibody-producing B cells which can protect from severe disease symptoms upon infection.
- Published
- 2018
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