1. In Vitro and In Vivo Characterization of a New Strain of Mosquito Flavivirus Derived from Culicoides.
- Author
-
Huang, Yi, Zhang, Hongqing, Li, Xiaodan, Zhao, Lu, Cai, Dirui, Wang, Shunlong, Ren, Nanjie, Ma, Haixia, Huang, Doudou, Wang, Fei, Yuan, Zhiming, Zhang, Bo, and Xia, Han
- Subjects
- *
CULICOIDES , *FLAVIVIRUSES , *VIRUS cloning , *AEDES aegypti , *VACCINE development , *MOSQUITOES , *ELECTRON microscopy , *CELL culture - Abstract
Mosquito-specific flaviviruses comprise a group of insect-specific viruses with a single positive RNA, which can affect the duplication of mosquito-borne viruses and the life growth of mosquitoes, and which have the potential to be developed as a vaccine platform for mosquito-borne viruses. In this study, a strain of mosquito flavivirus (MFV) YN15-283-02 was detected in Culicoides collected from Yunnan, China. The isolation of the purified MFV YN15-283-02 from cell culture failed, and the virus was then rescued by an infectious clone. To study the biological features of MFV YN15-283-02 in vitro and in vivo, electron microscopy, phylogenetic tree, and viral growth kinetic analyses were performed in both cell lines and mosquitoes. The rescued MFV (rMFV) YN15-283-02 duplicated and reached a peak in C6/36 cells at 6 d.p.i. with approximately 2 × 106 RNA copies/μL (RNA to cell ratio of 0.1), but without displaying a cytopathic effect. In addition, the infection rate for the rMFV in Ae.aegypti show a low level in both larvae (≤15%) and adult mosquitoes (≤12%). [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF