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Start Over Author "Lebedev GB" Journal voprosy virusologii
2 results on '"Lebedev GB"'

1. [Correlation between the receptor specificity of pandemic influenza A (H1N1)pdm09 virus strains isolated in 2009-2011 and the structure of the receptor-binding site and the probability of fatal primary viral pneumonia].

Author

L'vov DK, Shchelkanov MIu, Bovin NV, Malyshev NA, Chuchalin AG, Kolobukhina LV, Prilipov AG, Bogdanova VS, Al'khovskiĭ SV, Samokhvalov EI, Fediakina IT, Burtseva EI, Deriabin PG, Zhuravleva MM, Shevchenko ES, Lavrishcheva VV, L'vov DN, Proshina ES, Starikov NS, Morozova TN, Bazarova MV, Grigor'eva TA, Kirillov IM, Shidlovskaia EV, Kelli EI, Malikov VE, Iashkulov KB, Anan'ev VIu, Baranov NI, Gorelikov VN, Tsoi OV, Garbuz IuA, Reznik VI, Ivanov LI, Fedelesh IIu, Ponomarenko RA, Sakharova EA, Lebedev GB, and Maslov AI

Subjects
Amino Acid Substitution, Binding Sites, Hemagglutinins metabolism, Humans, Influenza A Virus, H1N1 Subtype genetics, Influenza A Virus, H1N1 Subtype pathogenicity, Influenza, Human complications, Influenza, Human transmission, Influenza, Human virology, Molecular Mimicry, Pandemics, Pneumonia, Viral etiology, Pneumonia, Viral transmission, Pneumonia, Viral virology, Polymers chemistry, Polymers metabolism, Probability, Receptors, Virus genetics, Receptors, Virus metabolism, Russia epidemiology, Sialoglycoproteins chemistry, Sialoglycoproteins metabolism, Survival Analysis, Viral Proteins metabolism, Hemagglutinins genetics, Influenza A Virus, H1N1 Subtype metabolism, Influenza, Human mortality, Pneumonia, Viral mortality, Receptors, Virus chemistry, Viral Proteins genetics
Abstract

The receptor specificity (RS) of pandemic influenza A(H1N1) pdm09 virus strains deposited into the State Collection of Viruses of the Russian Federation, D. I. Ivanovsky Research Institute of Virology, Ministry of Health and Social Development of Russia, in the 2009-2010 and 2010-2011 epidemic seasons to a panel of 9 sialoglycopolymers (SGP). The strains were divided into 3 groups according to the W(3/6) index proposed by the authors, which was equal to the amount of reactivities to unbranched alpha2-3-SGP to that of reactivities to unbranched alphal-6-SGP: W(3/6) < or = 1.0; 1.0 < W(3/6) < or = 1.5. The W(3/6) < or = 1.5 group showed a predominance of a2-3-RS, attended by the high incidence of fatal primary viral pneumonias (FPVP) (60.0%) and amino acid replacements in the HA1 receptor-binding site (RBS) (80.0%): D222{G, N} and Q223R. The 1.0 < W(3/6) < or = 1.5 group was characterized by mixed alpha2-3/alpha2-6-RS with the incidence of FPVP (29.7%) and amino acid replacements in the HA1 RBS (40.5%) (D222{G, N, V} and Q223), respectively. In the W(3/6) < or = 1.0 group, alpha2-6-RS was prevalent, FPVPs were absent and amino acid replacements in HA1 RBS (D222{G, E}) were seen only in 6.0% of cases. The number of strains with increased specificity to alpha2-3-sialosides increased in the 2010-2011 epidemic season as compared to the previous season. With their further spread among the population, there may be a rise in cases of severe primary viral pneumonias with possible fatal outcomes, which can be, however, accompanied by a decrease in the capacity of mutants to air-dropwise transmission.

Published
2012

2. [A possible association of fatal pneumonia with mutations of pandemic influenza A/H1N1 sw1 virus in the receptor-binding site of the HA1 subunit].

Author

L'vov DK, Burtseva EI, Prilipov AG, Bogdanova VS, Shchelkanov MIu, Bovin NV, Samokhvalov EI, Fediakina IT, Deriabin PG, Kolobukhina LV, Shtyria IuA, Shevchenko ES, Malyshev NA, Merkulova LN, Bazarova MV, Maslov AI, Ishchenko NM, Iskhakova EA, Al'khovskiĭ SV, Grebennikova TV, Sadykova GK, L'vov DN, Zhuravleva MM, Iamnikova SS, Shliapnikova OV, Poglazov AB, Trushakova SV, Lavrishcheva VV, Aristova VA, Proshina ES, Vereshchagin NN, Kuz'michev AG, Iashkulov KB, Dzhambinov SD, Bushkieva BTs, Eliseeva SM, Bystrakov SI, Sokolova IA, Dzhaparidzhe NI, Ledenev IuA, Rosolovskiĭ AP, Gareev RV, Boldyreva VV, Anan'ev VIu, Baranov NI, Gorelikov VN, Garbuz IuA, Reznik VI, Ivanov LI, Zdanovskaia NN, Sergeeva NM, Podolianko IA, Elovskiĭ OV, Gromova MA, Kalaeva EE, Grigor'ev SN, Eremeeva IuV, Dovgal' MV, Fedelesh IIu, Sakharova EA, Burtnik VI, Avdoshina LN, Shapiro NP, Maslov DV, Ianovich VA, Ott VA, and Lebedev GB

Subjects
Binding Sites genetics, Female, Hemagglutinin Glycoproteins, Influenza Virus metabolism, Humans, Influenza A Virus, H1N1 Subtype pathogenicity, Influenza, Human mortality, Lung virology, Male, Pneumonia, Viral mortality, Polymerase Chain Reaction, Pregnancy, Pregnancy Complications, Infectious mortality, Protein Subunits metabolism, Receptors, Virus metabolism, Russia epidemiology, Sequence Analysis, Protein, Virulence, Disease Outbreaks, Hemagglutinin Glycoproteins, Influenza Virus genetics, Influenza A Virus, H1N1 Subtype genetics, Influenza, Human epidemiology, Influenza, Human virology, Pneumonia, Viral epidemiology, Pneumonia, Viral virology, Pregnancy Complications, Infectious epidemiology, Pregnancy Complications, Infectious virology, Protein Subunits genetics
Abstract

The paper gives the results of sequence analysis of 150 positive samples in real-time RT-PCR, including 47 autopsy materials from patients (including 10 pregnant women), who died from fatal pneumonia mainly in November-December 2009, in whom the lifetime etiological diagnosis had not been made and hence no early etiotropic therapy performed. 70% of the primary materials from the deceased patients were found to have pandemic influenza A(H1N1) v mutants in the lung tissue with D222G (15%), D222N (15%), D222E (2%) substitutions, as well as a mixture of mutants (38%). Nasopharyngeal lavages from 3 Chukotka deceased patients exhibited only consensus (nonmutant) D222 virus variants; there was a mixture of consensus and mutant virus variants in the trachea and a mixture of mutant ones in the lung. Preliminary data from the study of the interaction of the hemagglutinin of two strains having D222G and D222N mutations with 9 oligosaccharides imitating the variants of cell receptors for influenza A virus suggest that there is a double receptor specificity for alpha2'-3' and alpha2'-6'-sialosides with a preponderance of alpha2'-3'-specificity. Further spread of the mutants that have acquired a high virulence and preserved their capacity for the respiratory route of human infection may lead to the situation similar to that seen in the 1918-1919 pandemic. Another scenario for evolution of the virus is to preserve its receptor specificity for alpha2'-3'-sialosides and high virulence with losses of alpha2'-6' specificity and capacity for aerosol transmission, by damping the pandemic.

Published
2010

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