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Humic acid (HA) in compost has received widespread attention for its high redox activity, which can mediate the degradation of organic pollution and the passivation of heavy metals in the environment. Hyperthermophilic composting (HTC) can accelerate HA formation. However, few studies have examined whether and how the structures of different organics affect the formation of the HA and HA redox structure at the molecular level in HTC. Detailed molecular information and the redox capacity (electron transfer capacity, ETC) of HA in HTC and thermophilic composting (TC) were characterized using pyrolysis gas chromatography/mass spectrometry and the electrochemical method, respectively. HTC promoted the formation of redox structure, leading to the improvement of the ETC of HA. Aromatics and N-containing compounds were mainly derived from protein components, and the rate at which they were transferred into HA was accelerated in HTC, while the relative abundance of lipids decreased. Partial least squares regression and correlation analysis demonstrated that protein-derived compounds were the key factor determining the HA redox capacity. Finally, partial least squares path modeling suggested that the influence mechanism of protein-derived structures on HA redox capacity might differ in HTC and TC. HTC may promote the relative abundance of N-containing components into the C-skeleton and accelerate the accumulation of the aromatic products, thereby improve the HA redox capacity. These findings provided new insight into how the redox capacity of the HA in compost could be improved and how compost products could be prepared for use in environmental remediation., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Application of thermophilic composting (TC) is limited due to poor efficiency and long composting period. Hyperthermophilic composting (HTC) could effectively overcome this defect. Here, the transformation of humic acid (HA) in both HTC and TC was characterized and compared to investigate the roles of HTC toward accelerating the formation of HA. In HTC, the highest temperature was 96.6 °C, and the hyperthermophilic and thermophilic phases exceed 18 days. The degree of polymerization (DP) in HTC increased to 1.27 on day 27, while it only increased to 1.15 at the end of TC. The elemental composition of the HA in HTC showed higher O atomic content (36.3%) and lower C/N atomic ratio (6.5) compared with TC. These changes indicated that HTC could significantly accelerate oxidized and polycondensed reactions for HA formation, which resulted in the shortening of composting period to 27 days. The maximum fluorescence intensity (F max ) of humic-like components were achieved faster in HTC (F max  = 1649.9) than in TC (F max  = 1316.9), implying that HTC promoted the polycondensation of small molecular components to form HA with larger molecular weight and higher degree of aromatization. Two-dimensional FTIR correlation spectroscopy (2D-FTIR-COS) analysis demonstrated that HTC prevented the HA precursor from condensing before it was deeply oxidized, and increased the content of small molecules rich in carboxyl moieties. Based on the evolution of the molecular structure of HA, the level of oxidation of HA precursors was a key factor to determine the degree of polymerization and the degree of HA humification., (Copyright © 2020 Elsevier Ltd. All rights reserved.)