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• A novel BACP technology was devised for p -CNB degradation in groundwater. • The PPR method in BACP notably accelerated cathodic biofilm formation. • The degradation mechanism of p -CNB within BACP technology was systematically elucidated. • Stability, safety, and environmental impact of p -CNB degradation in BACP were systematically assessed. The consistent presence of p -chloronitrobenzene (p -CNB) in groundwater has raised concerns regarding its potential harm. In this study, we developed a biocathode-anode cascade system in a permeable reactive barrier (BACP), integrating biological electrochemical system (BES) with permeable reactive barrier (PRB), to address the degradation of p -CNB in the groundwater. BACP efficiently accelerated the formation of biofilms on both the anode and cathode using the polar periodical reversal method, proving more conducive to biofilm development. Notably, BACP demonstrated a remarkable p -CNB removal efficiency of 94.76 % and a dechlorination efficiency of 64.22 % under a voltage of 0.5 V, surpassing the results achieved through traditional electrochemical and biological treatment processes. Cyclic voltammetric results highlighted the primary contributing factor as the synergistic effect between the bioanode and biocathode. It is speculated that this system primarily relies on bioelectrocatalytic reduction as the predominant process for p -CNB removal, followed by subsequent dechlorination. Furthermore, electrochemical and microbiological tests demonstrated that BACP exhibited optimal electron transfer efficiency and selective microbial enrichment ability under a voltage of 0.3–0.5 V. Additionally, we investigated the operational strategy for initiating BACP in engineering applications. The results showed that directly introducing BACP technology effectively enhanced microbial film formation and pollutant removal performance. [Display omitted] [ABSTRACT FROM AUTHOR]

• Fe(II)/Fe(III) ratio of the added magnetite is key to microbial community impact. • Varied Fe(II) proportion enriched specific nitrogen metabolism genes in Geobacter. • Magnetite influences species selection based on iron metabolic potential. • Magnetite enhances interspecies cooperation among truncated denitrifiers. • Magnetite promotes functionalization and modularization of denitrifying consortia. Conductive materials, such as magnetite, are recognized for their ability to enhance electron transfer and stimulate microbial metabolic activities. This study aimed to elucidate the metabolic potential and species interactions of dominant microbial species within complex communities influenced by magnetite. It indicated that the optimal dosage of magnetite at 4.5 mg/cm², would significantly improve denitrification efficiency and then reduce the time for removing 50 mg/L nitrate by 24.33 %. This enhancement was attributed to the reduced charge transfer resistance and the promoted formation of extracellular polymeric substances (EPS) facilitated by magnetite. Metagenomic analysis revealed that magnetite addition mitigated the competition among truncated denitrifiers for downstream nitrogen species, diminished the contribution of bacteria with complete nitrogen metabolism pathways to denitrification, and fostered a transition towards co-denitrification through interspecies cooperation, consequently leading to decreased nitrite accumulation and increased tolerance to nitrate shock loads. Furthermore, an in-depth study on a key species, Geobacter anodireducens JN93 within the bioelectrochemical system revealed that while magnetite with varying Fe(II) and Fe(III) ratios improved denitrification performance, the metabolic potential of Geobacter sp. varied for different nitrogen metabolism pathways. Collectively, this research provides insights into the microecological effects of magnetite on denitrifying consortia by shifting interspecific interactions via enhanced electron transfer. [Display omitted] [ABSTRACT FROM AUTHOR]

• A novel approach for energy- and catalyst-free activation of peracetic acid (PAA). • Bioelectrochemical activation of PAA yielded •OH at a 3:1 ratio to CH 3 C(O)O•. • •OH, CH 3 C(O)O• reacting with PAA to form CH 3 C(O)OO• contributes 68.1 % SMX removal. • The transformation byproducts of SMX and their toxicities were assessed. • The new activation method validated with 21 typical pharmaceuticals at neutral pH. Peracetic acid (PAA) has received increasing attention as an alternative oxidant for wastewater treatment. However, existing processes for PAA activation to generate reactive species typically require external energy input (e.g., electrically and UV-mediated activation) or catalysts (e.g., Co2+), inevitably increasing treatment costs or introducing potential new contaminants that necessitate additional removal. In this work, we developed a catalyst-free, self-sustaining bioelectrochemical approach within a two-chamber bioelectrochemical system (BES), where a cathode electrode in-situ activates PAA using renewable biogenic electrons generated by anodic exoelectrogens (e.g., Geobacter) degrading biodegradable organic matter (e.g., acetic acid) in wastewater at the anode. This innovative BES-PAA technique achieved 98 % and 81 % removal of 2 µM sulfamethoxazole (SMX) in two hours at pH 2 (cation exchange membrane) and pH 6 (bipolar membrane) using 100 μM PAA without external voltage. Mechanistic studies, including radical quenching, molecular probe validation, electron spin resonance (ESR) experiments, and density functional theory (DFT) calculations, revealed that SMX degradation was driven by reactive species generated via biogenic electron-mediated O O cleavage of PAA, with CH 3 C(O)OO• contributing 68.1 %, •OH of 18.4 %, and CH 3 C(O)O• of 9.4 %, where initial formation of •OH and CH 3 C(O)O• rapidly reacts with PAA to produce CH 3 C(O)OO•. The presence of common water constituents such as anions (e.g., Cl−, NO 3 −, and H 2 PO 4 −) and humic acid (HA) significantly hinders SMX removal via the BES-PAA technique, whereas CO 3 2− and HCO 3 − ions have a comparatively minor impact. Additionally, the study investigated the removal of various pharmaceuticals present in secondary treated municipal wastewater, attributing differences in removal efficiency to the selective action of CH 3 C(O)OO•. This research demonstrates a novel PAA activation method that is ecologically benign, inexpensive, and capable of overcoming catalyst deactivation and secondary pollution issues. [Display omitted] [ABSTRACT FROM AUTHOR]

• Successful transition of electro-bioremediation from laboratory to on-site pilot plant. • Achieved maximum nitrate reduction rate of 0.89 ± 0.09 kg NO 3 - m-3 d-1 at 2.0 h HRT cat. • Collaborative microbial community ensures full NO 3 - reduction to N 2. • Competitive operational cost of 0.4 € m-3 for on-site electro-bioremediation. The potential of nitrate electro-bioremediation has been fully demonstrated at the laboratory scale, although it has not yet been fully implemented due to the challenges associated with scaling-up bioelectrochemical reactors and their on-site operation. This study describes the initial start-up and subsequent stable operation of an electro-bioremediation pilot plant for the treatment of nitrate-contaminated groundwater on-site (Navata site, Spain). The pilot plant was operated under continuous flow mode for 3 months, producing an effluent suitable for drinking water in terms of nitrates and nitrites (<50 mg NO 3 - L-1; 0 mg NO 2 - L-1). A maximum nitrate removal rate of 0.9 ± 0.1 kg NO 3 - m-3 d-1 (efficiency 82 ± 18 %) was achieved at a cathodic hydraulic retention time (HRT cat) of 2.0 h with a competitive energy consumption of 4.3 ± 0.4 kWh kg-1 NO 3 -. Under these conditions, the techno-economic analysis estimated an operational cost of 0.40 € m-3. Simultaneously, microbiological analyses revealed structural heterogeneity in the reactor, with denitrification functionality concentrated predominantly from the centre to the upper section of the reactor. The most abundant groups were Pseudomonadaceae, Rhizobiaceae, Gallionellaceae , and Xanthomonadaceae. In conclusion, this pilot plant represents a significant advancement in implementing this technology on a larger scale, validating its effectiveness in terms of nitrate removal and cost-effectiveness. Moreover, the results validate the electro-bioremediation in a real environment and encourage further investigation of its potential as a water treatment. [Display omitted] [ABSTRACT FROM AUTHOR]

• A single-chamber up-flow anaerobic bioelectrochemical reactor (UBER) was designed. • The UBER realized biogenic S0 recovery from antibiotic production wastewater. • 0.8 V was the optimal voltage of UBER with the highest operational performance. • The bio-anode was proved to lower energy barrier and enhance electron transfer. • C -type cytochromes served as the crucial electron transfer mediator. The high-concentration sulfate (SO 4 2 −) in the antibiotic production wastewater hinders the anerobic methanogenic process and also proposes possible environmental risk. In this study, a novel single-chamber up-flow anaerobic bioelectrochemical reactor (UBER) was designed to realize simultaneous SO 4 2 − removal and elemental sulfur (S0) recovery. With the carbon felt, the cathode was installed underneath and the anode above to meet the different biological niches for sulfate reducing bacteria (SRB) and sulfur oxidizing bacteria (SOB). The bio-anode UBER (B-UBER) demonstrated a much higher average SO 4 2 − removal rate (SRR) of 113.2 ± 5.7 mg SO 4 2 − − S L−1 d−1 coupled with a S0 production rate (SPR) of 54.4 ± 5.8 mg S0-S L−1 d−1 at the optimal voltage of 0.8 V than that in the abio-anode UBER (control reactor) (SRR = 86.6 ± 13.4 mg SO 4 2 − − S L−1 d−1; SPR = 25.5 ± 9.7 mg S0-S L−1 d−1) under long-term operation. A large amount of biogenic S0 (about 72.2 mg g−1 VSS) was recovered in the B-UBER. The bio-anode, dominated by Thiovirga (SOB genus) and Acinetobacter (electrochemically active bacteria genus), exhibited a higher current density, lower overpotential, and lower internal resistance. C -type cytochromes mainly served as the crucial electron transfer mediator for both direct and indirect electron transfer, so that significantly increasing electron transfer capacity and biogenic S0 recovery. The reaction pathways of the sulfur transformation in the B-UBER were hypothesized that SRB utilized acetate as the main electron donor for SO 4 2 − reduction in the cathode zone and SOB transferred electrons to the anode or oxygen to produce biogenic S0 in the anode zone. This study proved a new pathway for biogenic S0 recovery and sulfate removal from sulfate-laden antibiotic production wastewater using a well-designed single-chamber bioelectrochemical reactor. [ABSTRACT FROM AUTHOR]

• Efficient metronidazole (MNZ) removal was realized using S-BES. • High MNZ concentration inhibited sulfur metabolism and decreased SO2–4 removal. • Three degradation pathways of MNZ were discovered. • The synergistic reactions improved the performance of S-BES. The aim of this study was to investigate the removal of metronidazole (MNZ) from seawater using a bioelectrochemical system (BES). Single-chamber BES (i.e., S-BES) and dual-chamber BES (i.e., D-BES) were constructed with carbon brush as the anode and cathode. With the inoculum of sea mud and 2 g/L of glucose as the substrate in seawater, S-BES and D-BES were acclimated to test the MNZ removal. Results showed that S-BES could remove almost 100 % of 200 mg/L MNZ within 120 h and remain stable within 10 cycles of operation (∼50 d) under the applied voltage of 0.8 V. The MNZ removal reached ∼100 % and 60.2 % in the cathodic and anodic chambers of D-BES fed by 100 mg/L MNZ under 0.8 V, respectively. The MNZ concentration of 200 mg/L significantly inhibited the sulfur metabolism, decreased the ratio of live to dead cells in the electrode biofilms, and thus reduced the SO 4 2− removal in the S-BES. The MNZ degradation and S2– oxidation was mainly attributed to the cathodic and anodic biofilms of S-BES, respectively. Three degradation pathways of MNZ were proposed based on the identified intermediates and results of density functional theory calculations. The synergies among different genus species in the bacterial communities of biofilms, and between anodic and cathodic reactions could be responsible for the high performance of S-BES. Results from this study should be not only useful for the MNZ removal but also for effective MNZ inhibition of sulfate-reducing bacteria induced microbiologically influenced corrosion in seawater. [Display omitted] [ABSTRACT FROM AUTHOR]

• Multi-physics simulation guided the design and optimization of BES anodes. • Binary carbon anode was spatially assembled with carbon mesh skeleton and GAC. • Directional electron transfer and enhanced microbe accommodation were synergized. • Binary carbon anode improved wastewater energy conversion with cost-effectiveness. Bioelectrochemical systems (BESs) hold prospects in wastewater energy and resource recovery. Anode optimization is important for simultaneous enhancement of wastewater energy conversion and effluent quality in BESs. In this study, a multi-physics model coupling fluid flow, organic degradation and electrochemical process was constructed to guide the design and optimization of BES anodes. Based on the multi-physics simulation, spatially-assembled binary carbon anodes composed of three-dimensional carbon mesh skeleton and granular activated carbon were proposed and established. The granular activated carbon conducive to microbe accommodation played a vital role in improving effluent water quality, while the carbon mesh skeleton favoring electron collection and transfer could enhance the bioelectricity output. With an average chemical oxygen demand (COD) removal rate of 0.442 kg m−3 d−1, a maximum power density of 20.6 W m−3 was achieved in the optimized composite anode BES, which was 25% and 154% higher than carbon mesh skeleton BES and granular activated carbon BES. Electroactive bacteria were enriched in composite anodes and performed important functions related to microbial metabolism and energy production. The spatially-assembled binary carbon anode with low carbon mesh packing density was more cost-effective with a daily energy output per anode cost of 221 J d−1 RMB−1. This study not only provides a cost-efficient alternative anode to simultaneously improve organic degradation and power generation performance, but also demonstrates the potential of multi-physics simulation in offering theoretical support and prediction for BES configuration design as well as optimization. [Display omitted] [ABSTRACT FROM AUTHOR]

The extracellular electron transfer (EET) efficiency in bioelectrochemical systems has been proven to be dependent on anode potentials. To explore the underlying mechanism, previous studies have mainly focused on EET conduit and bacterial biomass but rarely concerned with the role of extracellular polymeric substances (EPS) surrounding electroactive cells. In this study, the response of Geobacter biofilms to anode potentials was investigated with a special emphasis on the mechanistic role of EPS. The electrochemical activities and cell viabilities of Geobacter soli biofilms were simultaneously attenuated at 0.4 and 0.6 V compared to −0.2 and 0 V. It was found that the biofilms (especially the biofilm region closer to electrode surface) grown at −0.2 and 0 V produced relatively more extracellular redox-active proteins and less extracellular polysaccharides, which conferred higher electron accepting/donating capacities to EPS and consequently facilitated EET. Meanwhile, electrically nonconductive extracellular polysaccharide-dominated interior layers were formed in the biofilms grown at 0.4 and 0.6 V, which limited direct EET but might serve as physical barriers for protecting cells in these biofilms from the increasing stress by poised electrodes. These results demonstrated that the production of EPS under different anode potentials might be finely regulated by cells to keep balance between EET efficiency and cell-protection. This study provides a new insight to investigate the Geobacter biofilms coping with various environments, and is useful for optimizing electrochemical activity of anode biofilms. Image 1 • Highest EET and cell viability observed in biofilms grown at −0.2 and 0 V. • Heterogeneity is elucidated in spatial distribution of both viable cells and EPS. • Electrochemical activity of biofilm is positively related to redox activity of EPS. • Polysaccharide-dominated interior layers of EPS in 0.4 and 0.6 V biofilms hinder EET. [ABSTRACT FROM AUTHOR]

Abstract Carbon-rich waste materials (solid, liquid, or gaseous) are largely considered to be a burden on society due to the large capital and energy costs for their treatment and disposal. However, solid and liquid organic wastes have inherent energy and value, and similar as waste CO 2 gas they can be reused to produce value-added chemicals and materials. There has been a paradigm shift towards developing a closed loop, biorefinery approach for the valorization of these wastes into value-added products, and such an approach enables a more carbon-efficient and circular economy. This review quantitatively analyzes the state-of-the-art of the emerging microbial electrochemical technology (MET) platform and provides critical perspectives on research advancement and technology development. The review offers side-by-side comparison between microbial electrosynthesis (MES) and electro-fermentation (EF) processes in terms of principles, key performance metrics, data analysis, and microorganisms. The study also summarizes all the processes and products that have been developed using MES and EF to date for organic waste and CO 2 valorization. It finally identifies the technological and economic potentials and challenges on future system development. Graphical abstract Image 1 Highlights • Current status summary on CO 2 and organic waste valorization by MET. • A comprehensive and quantitative on this technology platform. • Side-by-side comparison of microbial electrosynthesis and electro-fermentation. [ABSTRACT FROM AUTHOR]

Abstract For over a century, anaerobic digestion has been a key technology in stabilizing organic waste streams, while at the same time enabling the recovery of energy. The anticipated transition to a bio-based economy will only increase the quantity and diversity of organic waste streams to be treated, and, at the same time, increase the demand for additional and effective resource recovery schemes for nutrients and organic matter. The performance of anaerobic digestion can be supported and enhanced by (bio)electrochemical systems in a wide variety of hybrid technologies. Here, the possible benefits of combining anaerobic digestion with (bio)electrochemical systems were reviewed in terms of (1) process monitoring, control, and stabilization, (2) nutrient recovery, (3) effluent polishing, and (4) biogas upgrading. The interaction between microorganisms and electrodes with respect to niche creation is discussed, and the potential impact of this interaction on process performance is evaluated. The strength of combining anaerobic digestion with (bio)electrochemical technologies resides in the complementary character of both technologies, and this perspective was used to distinguish transient trends from schemes with potential for full-scale application. This is supported by an operational costs assessment, showing that the economic potential of combining anaerobic digestion with a (bio)electrochemical system is highly case-specific, and strongly depends on engineering challenges with respect to full-scale applications. Graphical abstract Image Highlights • Anaerobic digestion and (bio)electrochemical systems are complementary technologies. • Various hybrid technologies can improve the anaerobic digestion process. • Schemes with full-scale potential are distinguished from transient trends. • An operational cost assessment is essential for full-scale application. [ABSTRACT FROM AUTHOR]

A key challenge for osmotic membrane bioreactors (OMBRs) application is reverse solute flux and consequent salt accumulation in the feed side. Herein, a bioelectrochemical system (BES) was employed to drive reverse-fluxed solutes from the feed of an OMBR into a cathode compartment for recovery and subsequent reuse as a draw solute (DS). Compared to an OMBR without BES function, the present OMBR system enhanced water recovery from 925 to 1688 mL and increased the chemical oxygen demand (COD) removal efficiency from 40.2 ± 8.1 to 75.2 ± 3.3%, benefited from its lower anolyte conductivity of 9.0 mS cm −1 than that of the control system (24.1 mS cm −1 ). The CO 2 addition significantly improved the ammonia recovery rate to 93.3–116.7 g N m −3 h −1 (or 248.0–307.4 g N m −2 d −1 ), 12.1–14.5 times higher than that without CO 2 addition. The recovered DS was successfully applied to accomplish water extraction in the reuse test, and such a recovery/reuse process could result in a normalized water recovery of 3870 mL mol DS −1 or a DS usage of 0.26 mol L −1 (of the recovered water). The energy consumption of the system might be compensated by the production of bioenergy, and the net specific energy consumption was estimated to be 0.004–0.112 kWh m −3 wastewater, 0.007–0.179 kWh kg −1 removed COD, or 0.001–0.020 kWh kg −1 recovered NH 4 + -N. Those results have demonstrated that bioelectrochemical processes can be an effective approach for in situ mitigation of reverse-fluxed solute in OMBR and recovering “the lost DS” towards both reuse and reduced operational expense. [ABSTRACT FROM AUTHOR]

Pharmaceutical wastewaters containing antibiotics and high salinity can damage traditional biological treatment and result in the proliferation of antibiotic resistance genes (ARGs). Bioelectrochemical system (BES) is a promising approach for treating pharmaceutical wastewater. However, the fate of ARGs in BES and their correlations with microbial communities and horizontal genes transfer are unknown. In this study, we investigated the response of ARGs to bio-electrochemical treatment of chloramphenicol wastewater and their potential hosts under different salinities. Three ARGs encoding efflux pump ( cmlA , floR and tetC ), one class 1 integron integrase encoding gene ( intI1 ), and sul1 gene (associate with intI1 ) were analyzed. Correlation analysis between microbial community and ARGs revealed that the abundances of potential hosts of ARGs were strongly affected by salinity, which further determined the alteration in ARGs abundances under different salinities. There were no significant correlations between ARGs and intI1 , indicating that horizontal gene transfer was not related to the important changes in ARGs. Moreover, the chloramphenicol removal efficiency was enhanced under a moderate salinity, attributed to the altered microbial community driven by salinity. Therefore, microbial community shift is the major factor for the changes of ARGs and chloramphenicol removal efficiency in BES under different salinities. This study provides new insights on the mechanisms underlying the alteration of ARGs in BES treating high-salinity pharmaceutical wastewater. [ABSTRACT FROM AUTHOR]

Bioelectrochemical systems (BES) can accomplish simultaneous wastewater treatment and resource recovery via interactions between microbes and electrodes. Often deemed as “energy efficient” technologies, BES have not been well evaluated for their energy performance, such as energy production and consumption. In this work, we have conducted a review and analysis of energy balance in BES with parameters like normalized energy recovery, specific energy consumption, and net energy production. Several BES representatives based on their functions were selected for analysis, including direct electricity generation in microbial fuel cells, hydrogen production in microbial electrolysis cells, nitrogen recovery in BES, chemical production in microbial electrosynthesis cells, and desalination in microbial desalination cells. Energy performance was normalized to water volume (kWh m −3 ), organic removal (kWh kg COD −1 ), nitrogen recovery (kWh kg N −1 ), chemical production (kWh kg −1 ), or removed salt during desalination (kWh kg −1 ). The key operating factors such as pumping system (recirculation/feeding pumps) and external power supply were discussed for their effects on energy performance. This is an in-depth analysis of energy performance of various BES and expected to encourage more thinking, analysis, and presentation of energy data towards appropriate research and development of BES technology for resource recovery from wastewater. [ABSTRACT FROM AUTHOR]

Removal of nitrogen, mainly in form of ammonium (NH 4 + ), in wastewater treatment plants (WWTPs) is a highly energy demanding process, mainly due to aeration. It causes costs of about half a million Euros per year in an average European WWTP. Alternative, more economical technologies for the removal of nitrogen compounds from wastewater are required. This study proves the complete anoxic conversion of ammonium (NH 4 + ) to dinitrogen gas (N 2 ) in continuously operated bioelectrochemical systems at the litre-scale. The removal rate is comparable to conventional WWTPs with 35 ± 10 g N m −3 d −1 with low accumulation of NO 2 − , NO 3 − , N 2 O. In contrast to classical aerobic nitrification, the energy consumption is considerable lower (1.16 ± 0.21 kWh kg −1 N, being more than 35 times less than for the conventional wastewater treatment). Biotic and abiotic control experiments confirmed that the anoxic nitrification was an electrochemical biological process mainly performed by Nitrosomonas with hydroxylamine as the main substrate (mid-point potential, E ox = +0.67 ± 0.08 V vs. SHE). This article proves the technical feasibility and reduction of costs for ammonium removal from wastewater, investigates the underlying mechanisms and discusses future engineering needs. [ABSTRACT FROM AUTHOR]

Bioelectrochemical systems can effectively degrade antibiotics, but there is the need to better understand the fate of antibiotic resistance bacteria and antibiotic resistance genes during the bioelectrochemical degradation of antibiotics. In this study, a BES was developed as a platform to investigate the fate of chloramphenicol resistance bacteria (CRB) and the expression of chloramphenicol resistance genes (CRGs) under different operating conditions during chloramphenicol biodegradation. The results indicated that chloramphenicol was effectively removed and chloramphenicol removal efficiency could be improved under less chloramphenicol concentration and more negative cathode potential. Higher chloramphenicol concentration enhanced the enrichment of CRB and expression of CRGs. Furthermore, the abundances of CRB were enhanced under more negative cathode potential, the expression of CRGs under less negative cathode potential were induced. However, both the enrichment of CRB and expression of CRGs could be moderated under a medium cathode potential. This result could provide the scientific reference for research about the fate of antibiotic resistance genes in bioelectrochemical systems. [ABSTRACT FROM AUTHOR]

• Simultaneous denitrification and heavy metals recovery were investigated in BES. • 500 mg/L NO 3 −-N decreased redox activities and power generation of BES. • The NO 3 −-N removal efficiency was above 90% for 100 and 250 mg/L concentrations. • BES inoculated with Castellaniella performed denitrification and metals recovery. Although Castellaniella species are crucial for denitrification, there is no report on their capacity to carry out denitrification and anode respiration simultaneously in a bioelectrochemical system (BES). Herein, the ability of a mixed inoculum of electricigenic Castellaniella species to perform simultaneous denitrification and anode respiration coupled with cathodic metals recovery was investigated in a BES. Results showed that 500 mg/L NO 3 −-N significantly decreased power generation, whereas 100 and 250 mg/L NO 3 −-N had a lesser impact. The single-chamber MFCs (SCMFCs) fed with 100 and 250 mg/L NO 3 −-N concentrations achieved a removal efficiency higher than 90% in all cycles. In contrast, the removal efficiency in the SCMFCs declined dramatically at 500 mg/L NO 3 −-N, which might be attributable to decreased microbial viability as revealed by SEM and CLSM. EPS protein content and enzymatic activities of the biofilms decreased significantly at this concentration. Cyclic voltammetry results revealed that the 500 mg/L NO 3 −-N concentration decreased the redox activities of anodic biofilms, while electrochemical impedance spectroscopy showed that the internal resistance of the SCMFCs at this concentration increased significantly. In addition, BES inoculated with the Castellaniella species was able to simultaneously perform heterotrophic anodic denitrification and cathodic metals recovery from real wastewater. The BES attained Cu2+, Hg2+, Pb2+, and Zn2+ removal efficiencies of 99.86 ± 0.10%, 99.98 ± 0.014%, 99.98 ± 0.01%, and 99.17 ± 0.30%, respectively, from the real wastewater. Cu2+ was bio-electrochemically reduced to Cu0 and Cu 2 O, whereas Hg0 and HgO constituted the Hg species recovered via bioelectrochemical reduction and chemical deposition, respectively. Furthermore, Pb2+ and Zn2+ were bio-electrochemically reduced to Pb0 and Zn0, respectively. Over 89% of NO 3 −-N was removed from the BES anolyte during the recovery of the metals. This research reveals promising denitrifying exoelectrogens for enhanced power generation, NO 3 −-N removal, and heavy metals recovery in BES. [Display omitted] [ABSTRACT FROM AUTHOR]

A major challenge of employing bioelectrochemical system (BES) for reductively degrading recalcitrant contaminants in industrial wastewater is lacking sufficient electron donors. In this work, domestic wastewater (DW) was demonstrated to efficiently drive BES for implementing the decolorization of azo dye, acid orange 7 (AO7). Side benefit was the simultaneous treatment of DW. Decolorization efficiency in BES fed with DW (R DW ) was found to be comparable with that either fed with glucose (R Glu ) or acetate (R Ac ). Much lower reductant usage ratio was observed in R DW . As a result, when the ratio of electron donors to azo dye decreased to 4.4 mol COD mol −1 AO7, DE of R DW kept over 90% while DEs of R Ac and R Glu were significantly dropped due to the insufficient electrons donation. Besides serving as electron donor, DW was proved to also provide some conductivity and buffer capacity. Accordingly, DE of R DW was less deteriorated when fully removing the external buffer slats. This study comprehensively revealed the feasibility and superiority of DW as a cost-effective electron donor source in BES and brings this technology closer to the practice. [ABSTRACT FROM AUTHOR]

Bacterial extracellular electron transfer is one of the main bottlenecks in determining the efficiency of bioelectrochemical systems. Here, we report a photobioanode that combines carbon material with a photocatalyst (α-Fe 2 O 3 ), utilizing visible light to accelerate biofilm formation and extracellular electron transfer in bioelectrochemical systems. Cyclic voltammetric studies of this photobioanode revealed active electron transfer at the anode/biofilm interface. The charge-transfer resistance of the anode/biofilm was ca. 46.6 Ω, which is half that of the unmodified anode. In addition, the results of confocal laser scanning microscopy and bacterial community analysis indicate that the photobioanode and light can accelerate biofilm formation and enrich exoelectrogens. When equipped in photo-bioelectrochemical systems, the start-up time was shortened from about 2.5 days to 1.1 days. The maximum current density of photo-bioelectrochemical systems was almost twice that of a control bioelectrochemical system. In addition, the current density of the photo-bio-electrochemical cell (PBEC) showed almost no decrease after being subjected to 40 d of illumination. This photobioanode is therefore a cost-effective, energy-clean, environment-friendly anode with high electrocatalytic activity and long-term stability, which has broad prospects in various processes, including wastewater treatment, bioelectricity generation, bioelectricity synthesis, and hydrogen production. [ABSTRACT FROM AUTHOR]

Anaerobic digestion at low temperature is an attractive technology especially in moderate climates, however, low temperature results in low microbial activity and low rates of methane formation. This study investigated if bioelectrochemical systems (BESs) can enhance methane production from organic matter in low-temperature anaerobic digestion (AD). A bioelectrochemical reactor was operated with granular activated carbon as electrodes at 10 °C. Our results showed that bioelectrochemical systems can enhance CH 4 yield, accelerate CH 4 production rate and increase acetate removal efficiency at 10 °C. The highest CH 4 yield of 31 mg CH 4 -COD/g VSS was achieved in the combined BES-AD system at a cathode potential of −0.90 V (Ag/AgCl), which was 5.3–6.6 times higher than that in the AD reactor at 10 °C. CH 4 production rate achieved in the combined BES-AD system at 10 °C was only slightly lower than that in the AD reactor at 30 °C. The presence of an external circuit between the acetate-oxidizing bioanode and methane-producing cathode provided an alternative pathway from acetate via electrons to methane, potentially via hydrogen. This alternative pathway seems to result in higher CH 4 production rates at low temperature compared with traditional methanogenesis from acetate. Integration of BES with AD could therefore be an attractive alternative strategy to enhance the performance of anaerobic digestion in cold areas. [ABSTRACT FROM AUTHOR]

• Previous reviews mainly focused on the functions and applications of models. • Previous reviews usually neglected the strategies used to build a model. • Physiochemical principles and mathematical methods in models are analyzed in this review. Bioelectrochemical systems (BESs) are promising devices for wastewater treatment and bio-energy production. Since various processes are interacted and affect the overall performance of the device, the development of theoretical modeling is an efficient approach to understand the fundamental mechanisms that govern the performance of the BES. This review aims to summarize the physiochemical principle and mathematical method in BES models, which is of great importance for the establishment of an accurate model while has received little attention in previous reviews. In this review, we begin with a classification of existing models including bioelectrochemical models, electronic models, and machine learning models. Subsequently, physiochemical principles and mathematical methods in models are discussed from two aspects: one is the description of methodology how to build a framework for models, and the other is to further review additional methods that can enrich model functions. Finally, the advantages/disadvantages, extended applications, and perspectives of models are discussed. It is expected that this review can provide a viewpoint from methodologies to understand BES models. [Display omitted] [ABSTRACT FROM AUTHOR]

• Anode potentials favored the dominance of Electrogens in acetate competition • Anode potentials suppressed methanogenesis without additional chemical input • Electrogenesis-induced pH decrease assists suppressing methanogenesis • Anode potentials appears to stimulate sulfate reduction Methane emissions from the sewer system are considered to be a non-negligible source of aggravating the greenhouse effect. Meanwhile, the sewer system has long been plagued by sulfide-induced corrosion problems. This study explored the possibility of using a bioelectrochemical system to intensify the competition between electroactive bacteria, methanogens and sulfate-reducing bacteria, thereby reducing the production of methane and sulfide. Dual-chamber bioelectrochemical reactors were constructed and operated in fed-batch mode with the coexistence of Electroactive bacteria, Methanogenic archaea and Sulfate-reducing bacteria. Acetate was supplied as the sole carbon source. The results indicated that electrogenesis induced by the anode potentials of -0.42 V and -0.2 V (vs. Ag/AgCl) had advantages over methanogenesis and sulfidogenesis in consuming acetate. The stimulated electrogenesis by anode potentials resulted in a decrease in pH. Methane production was suppressed in the reactors with anode potentials of -0.42 and -0.2 V compared to open circuit controls. In contrast to methane, the capacity for sulfide production was facilitated in the reactors with the anode potentials of -0.42 V and -0.2 V compared to open circuit controls. 16s rRNA gene analysis showed that Geobacter was the most abundant genus on the anode biofilm in the anode potential-controlled reactor, while acetoclastic methanogens dominated in open circuit controls. Methanosaeta and Methanosarcina were the most abundant methanogens in open circuit controls. Collectively, our study demonstrates that the use of electrodes with anode potential control can help to control methane emissions, but could not yet prevent sulfide production, which requires further research. [Display omitted] [ABSTRACT FROM AUTHOR]

This study examined the effects of the microbial electrolysis cell (MEC) reactions on anaerobic digestion of waste activated sludge from municipal wastewater treatment under ambient temperature conditions (22–23 °C). Two lab-scale digesters, a control anaerobic digester and an electrically-assisted digester (EAD – equipped with a MEC bioanode and cathode) were operated under three solids retention times (SRT = 7, 10 and 14 days) at 22.5 ± 0.5 °C. A numerical model was also built by including the MEC electrode reactions in Anaerobic Digestion Model No.1. In experiments, the EAD showed reduced concentration of acetic acid, propionic acid, n-butyric acid and iso-butyric acid. This improved performance of the EAD is thought to be achieved by direct oxidation of the short-chain fatty acids at the bioanode as well as indirect contribution of low acetic acid concentration to enhancing beta-oxidation. The VSS and COD removal was consistently higher in the EAD by 5–10% compared to the control digester for all SRT conditions at 22.5 ± 0.5 °C. When compared to mathematical model results, this additional COD removal in the EAD was equivalent to that which would be achieved with conventional digesters at mesophilic temperatures. The magnitude of electric current in the EAD was governed by the organic loading rate while conductivity and acetic acid concentration showed negligible effects on current generation. Very high methane content (∼95%) in the biogas from both the EAD and control digester implies that the waste activated sludge contained large amounts of lipids and other complex polymeric substances compared to primary sludge. [ABSTRACT FROM AUTHOR]

Ammonia and sulfate, which are prevalent pollutants in agricultural and industrial wastewaters, can cause serious inhibition in several biological treatment processes, such as anaerobic digestion. In this study, a novel bioelectrochemical approach termed bipolar bioelectrodialysis was developed to recover ammonia and sulfate from waste streams and thereby counteracting their toxicity during anaerobic digestion. Furthermore, hydrogen production and wastewater treatment were also accomplished. At an applied voltage of 1.2 V, nitrogen and sulfate fluxes of 5.1 g NH 4 + -N/m 2 /d and 18.9 g SO 4 2 − /m 2 /d were obtained, resulting in a Coulombic and current efficiencies of 23.6% and 77.4%, respectively. Meanwhile, H 2 production of 0.29 L/L/d was achieved. Gas recirculation at the cathode increased the nitrogen and sulfate fluxes by 2.3 times. The applied voltage, initial (NH 4 ) 2 SO 4 concentrations and coexistence of other ions were affecting the system performance. The energy balance revealed that net energy (≥16.8 kWh/kg-N recovered or ≥4.8 kWh/kg-H 2 SO 4 recovered) was produced at all the applied voltages (0.8–1.4 V). Furthermore, the applicability of bipolar bioelectrodialysis was successfully demonstrated with cattle manure. The results provide new possibilities for development of cost-effective technologies, capable of waste resources recovery and renewable energy production. [ABSTRACT FROM AUTHOR]

Volatile fatty acids (VFA) are organic compounds of great importance for various industries and environmental processes. Fermentation and anaerobic digestion of organic wastes are promising alternative technologies for VFA production. However, one of the major challenges is development of sustainable downstream technologies for VFA recovery. In this study, an innovative microbial bipolar electrodialysis cell (MBEDC) was developed to meet the challenge of waste-derived VFA recovery, produce hydrogen and alkali, and potentially treat wastewater. The MBEDC was operated in fed-batch mode. At an applied voltage of 1.2 V, a VFA recovery efficiency of 98.3%, H 2 of 18.4 mL and alkali production presented as pH of 12.64 were obtained using synthetic fermentation broth. The applied voltage, initial VFA concentrations and composition were affecting the VFA recovery. The energy balance revealed that net energy (5.20–6.86 kWh/kg-VFA recovered) was produced at all the applied voltages (0.8–1.4 V). The coexistence of other anionic species had no negative effect on VFA transportation. The VFA concentration was increased 2.96 times after three consecutive batches. Furthermore, the applicability of MBEDC was successfully verified with digestate. These results demonstrate for the first time the possibility of a new method for waste-derived VFA recovery and valuable products production that uses wastewater as fuel and bacteria as catalyst. [ABSTRACT FROM AUTHOR]

The use of synthetic wastewater containing carbon sources of different complexity (glycerol, milk and starch) was evaluated in single chamber microbial electrolysis cell (MEC) for hydrogen production. The growth of an anodic syntrophic consortium between fermentative and anode respiring bacteria was operationally enhanced and increased the opportunities of these complex substrates to be treated with this technology. During inoculation, current intensities achieved in single chamber microbial fuel cells were 50, 62.5, and 9 A m −3 for glycerol, milk and starch respectively. Both current intensities and coulombic efficiencies were higher than other values reported in previous works. The simultaneous degradation of the three complex substrates favored power production and COD removal. After three months in MEC operation, hydrogen production was only sustained with milk as a single substrate and with the simultaneous degradation of the three substrates. The later had the best results in terms of current intensity (150 A m −3 ), hydrogen production (0.94 m 3 m −3 d −1 ) and cathodic gas recovery (91%) at an applied voltage of 0.8 V. Glycerol and starch as substrates in MEC could not avoid the complete proliferation of hydrogen scavengers, even under low hydrogen retention time conditions induced by continuous nitrogen sparging. [ABSTRACT FROM AUTHOR]

• The effect of Ni2+ on the performance of anodic EABs was investigated. • Ni2+ decreased microbial viability and resulted in a shift in microbial community. • Ni2+ decreased biocatalytic activity and power generation of BES. • Ni2+ resulted in a decrease in Geobacter and an increase in Desulfovibrio. • Ni2+ decreased the expression of extracellular electron transfer-related genes. The impact of nickel (Ni2+) on the performance of anodic electroactive biofilms (EABs) in the bioelectrochemical system (BES) was investigated in this study. Although it has been reported that Ni2+ influences microorganisms in a number of ways, it is unknown how its presence in the anode of a BES affects extracellular electron transfer (EET) of EABs, microbial viability, and the bacterial community. Results revealed that the addition of Ni2+ decreased power output from 673.24 ± 12.40 mW/m2 at 0 mg/L to 179.26 ± 9.05 mW/m2 at 80 mg/L. The metal and chemical oxygen demand removal efficiencies of the microbial fuel cells (MFCs) declined as Ni2+ concentration increased, which could be attributed to decreased microbial viability as revealed by SEM and CLSM. FTIR analysis revealed the involvement of various microbial biofilm functional groups, including hydroxyl, amides, methyl, amine, and carboxyl, in the uptake of Ni2+. The presence of Ni2+ on the anodic biofilms was confirmed by SEM-EDS and XPS analyses. CV demonstrated that the electron transfer performance of the anodic biofilms was negatively correlated with the various Ni2+ concentrations. EIS showed that the internal resistance of the MFCs increased with increasing Ni2+ concentration, resulting in a decrease in power output. High-throughput sequencing results revealed a decrease in Geobacter and an increase in Desulfovibrio in response to Ni2+ concentrations of 10, 20, 40, and 80 mg/L. Furthermore, the various Ni2+ concentrations decreased the expression of EET-related genes. The Ni2+-fed MFCs had a higher abundance of the nikR gene than the control group, which was important for Ni2+ resistance. This work advances our understanding of Ni2+ inhibition on EABs, as well as the concurrent removal of organic matter and Ni2+ from wastewater. [Display omitted] [ABSTRACT FROM AUTHOR]

Metal contaminated wastewater posts great health and environmental concerns, but it also provides opportunities for precious metal recovery, which may potentially make treatment processes more cost-effective and sustainable. Conventional metal recovery technologies include physical, chemical and biological methods, but they are generally energy and chemical intensive. The recent development of bioelectrochemical technology provides a new approach for efficient metal recovery, because it offers a flexible platform for both oxidation and reduction reaction oriented processes. While dozens of recent studies demonstrated the feasibility of the bioelectrochemical metal recovery concept, the mechanisms have been different and confusing. This study provides a review that summarizes and discusses the different fundamental mechanisms of metal conversion, with the aim of facilitating the scientific understanding and technology development. While the general approach of bioelectrochemical metal recovery is using metals as the electron acceptor in the cathode chamber and organic waste as the electron donor in the anode chamber, there are so far four mechanisms that have been reported: (1) direct metal recovery using abiotic cathodes; (2) metal recovery using abiotic cathodes supplemented by external power sources; (3) metal conversion using bio-cathodes; and (4) metal conversion using bio-cathodes supplemented by external power sources. [ABSTRACT FROM AUTHOR]

We demonstrated the ability of a bio-anode to fix dinitrogen (N 2 ), and confirmed that diazotrophs can be used to treat N-deficient wastewater in a bioelectrochemical system (BES). A two-compartment BES was fed with an N-deficient medium containing glucose for >200 days. The average glucose and COD removal at an anodic potential of +200 mV vs. Ag/AgCl was 100% and 76%, respectively. Glucose removal occurred via fermentation under open circuit (OC), with acetate as the key byproduct. Closing circuit remarkably reduced acetate accumulation, suggesting the biofilm could oxidise acetate under N-deficient conditions. Nitrogen fixation required an anode and glucose; removing either reduced N 2 fixation significantly. This suggests that diazotroph utilised glucose directly at the anode or indirectly through syntrophic interaction of an N 2 -fixing fermenter and an anodophile. The enriched biofilm was dominated (68%) by the genus Clostridium , members of which are known to be electrochemically active and capable of fixing N 2 . [ABSTRACT FROM AUTHOR]

Abstract: p-Fluoronitrobenzene (p-FNB) tends to accumulate in industrial effluents because of its recalcitrant properties. Approaches to the removal of p-FNB always encounter conflicts between treatment efficiency and economic efficiency. A bioelectrochemical system (BES) was established to facilitate the removal and mineralization of p-FNB. The treatment cost was reduced by using inexpensive electrode materials and reducing the electrical energy used. p-FNB was effectively removed using the BES, and the reaction rate was higher than the sum of the rates of two control systems, i.e., a biological system (BS) and an electrocatalytic system (ECS), by a maximum of 62.9% under a voltage of 1.4 V. The voltage is a crucial kinetic factor for the BES performance; as the voltage increased from 0 to 1.4 V, the reaction rate constants for p-FNB removal and defluorination increased from 0.0520 to 0.1811 h−1 and 0 to 0.0107 h−1. The synergistic effect of multistrains gave a TOC removal efficiency in the BES of about 34.05%, yet the removal efficiencies were low for the two control. The defluorination reaction rate was significantly slower than the p-FNB removal rate, which indicated that defluorination lagged p-FNB removal, and p-FNB transformation to p-fluoroaniline (p-FA) was the fastest step. The electrochemical assistance provided electrons and accelerated the electron transfer rate in the microbial reduction of p-FNB to p-FA. In this study, the critical voltage for defluorination in the BES was 0.8 V, which was approximately 0.2 V lower than that in the ECS. The decrease in the critical voltage for defluorination was based on the production of p-FA, which is more electrocatalytically activated. These results demonstrate the mechanism of efficient p-FNB removal and mineralization in a BES. [Copyright &y& Elsevier]

Abstract: Anaerobic digestion (AD) is a well-established technology for energy recovery from organic waste streams. Several studies noted that inserting a bioelectrochemical system (BES) inside an anaerobic digester can increase biogas output, however the mechanism behind this was not explored and primary controls were not executed. Here, we evaluated whether a BES could stabilize AD of molasses. Lab-scale digesters were operated in the presence or absence of electrodes, in open (no applied potential) and closed circuit conditions. In the control reactors without electrodes methane production decreased to 50% of the initial rate, while it remained stable in the reactors with electrodes, indicating a stabilizing effect. After 91 days of operation, the now colonized electrodes were introduced in the failing AD reactors to evaluate their remediating capacity. This resulted in an immediate increase in CH4 production and VFA removal. Although a current was generated in the BES operated in closed circuit, no direct effect of applied potential nor current was observed. A high abundance of Methanosaeta was detected on the electrodes, however irrespective of the applied cell potential. This study demonstrated that, in addition to other studies reporting only an increase in methane production, a BES can also remediate AD systems that exhibited process failure. However, the lack of difference between current driven and open circuit systems indicates that the key impact is through biomass retention, rather than electrochemical interaction with the electrodes. [Copyright &y& Elsevier]

Abstract: Electrochemical treatment of nitrate (NO3 −), nitrite (NO2 −) and mixtures of nitrate and nitrite was evaluated with microbial catalysts on a cathode in three different bioelectrochemical denitrification systems (BEDS). The removal rates and removal percentage of nitrogen (N) compounds varied during biotic and abiotic operations. The biotic cathode using NO3 −-N as an electron acceptor showed enhanced removal percentages (88%) compared to the operation with NO2 −-N (85%). The simultaneous reduction of NO3 −-N and NO2 −-N occurred in the operation with a mixture of N compounds. The bacterial diversity from the initial inoculum (return sludge) changed at the end of bioelectrochemical denitrification operation after 55 days. The microbial community composition was different depending on the type of electron acceptor. BEDS operation with NO3 −-N and NO2 −-N was enriched with Proteobacteria and Firmicutes respectively. BEDS with a mixture of N electron acceptors showed enrichment with Proteobacteria. There was no clear, distinct microbial community between the cathode biofilm and suspended biomass. [Copyright &y& Elsevier]

• pCNB inhibited the ammonia oxidation, plant and microbial growth in CW. • BES improved the tolerance of CW to pCNB by highly efficient degradation of pCNB. • ECW could significantly enhance TN and nitrate removal compared with CW. • Low voltage enhanced electroactive pCNB degradation bacteria growth in ECW. Constructed wetlands (CWs) integrated with the bioelectrochemical system (BES-CW) to stimulate bio-refractory compounds removal holds particular promise, owing to its inherent greater scale and well-recognized environmentally benign wastewater advanced purification technology. However, the knowledge regarding the feasibility and removal mechanisms, particularly the potential negative effects of biorefractory compounds on nitrogen removal performance for the CWs is far insufficient. This study performed a critical assessment by using BES-CW (ECW) and conventional CW (CW) to investigate the effects of p-Chloronitrobenzene (pCNB) on nitrogen transformations in CWs. The results showed that low concentration (1 mg·L−1) of pCNB would inhibit the ammonia oxidation in CWs, while ECW could improve its tolerance to pCNB to a certain level (8 mg·L−1) due to the high pCNB degradation efficiencies (2.5 times higher than CWs), accordingly, much higher TN and nitrate removal efficiencies were observed in ECWs, 81.71% - 96.82% (TN) higher than CWs, further leading to a lower N 2 O emission from ECWs than CWs. The main intermediate of pCNB degradation was p-Chloroaniline (pCAN) and the genera Geobacter and Propionimicrobium were consider to be the responsible pCNB degradation bacteria in the present study. However, too high concentration (20 mg·L−1) of pCNB would have a huge impact on ECW and CW, especially microbial biomass. Nevertheless, ECW could improve the 1.87 times higher microbial biomass than CW on the substrate. Accordingly, considerably higher functional gene abundance was observed in ECW. Therefore, the introduction of BES has great potential to ensure CW stability when treating industrial wastewater containing bio-refractory compounds. [Display omitted] [ABSTRACT FROM AUTHOR]

Abstract: Under autotrophic conditions, we investigated the effects of different current densities on bioelectrochemical denitrification (BED). In this study, nitrate consumption and nitrous oxide (N2O) production, microbial diversity and population dynamics, and denitrification pathway gene expressions were explored in continuous flow BED reactors at different current densities (0.2, 1, 5, 10 and 20 A/m2). We found that, under the autotrophic conditions, N2O accumulation was increased with increase in current density. The maximum rate of denitrification was 1.65 –N (g/NCCm3.h), and approximately 70% of the reduced N was accumulated as N2O. After each current density was applied, pyrosequencing of the expressed 16S rRNA genes amplified from the cathodic biofilms revealed that that 16 genera were active and in common at all currents, and that eight of those showed a statistically significant correlation with particular current densities. The relative expression of napA and narG was highest, whereas nosZ was low relative to its level in the inoculum suggesting that this could have contributed the high N2O accumulation. Kinetic analysis of nitrate reduction and N2O accumulation followed Michaelis–Menten kinetics. The V max for nitrate consumption and N2O accumulation were similar, however the K m values determined as A/m2 were not. This study provides better understanding of the community and kinetics of a current-fed, autotrophic, cathodic biofilm for evaluating its potential for scale-up and for N2O recovery. [Copyright &y& Elsevier]

Abstract: The effect of nitro-substituent on mononitrophenol (o-nitrophenol (ONP), m-nitrophenol (MNP) and p-nitrophenol (PNP)) reduction in a bioelectrochemical system (BES) was investigated in this study. The results show that the removal of all three nitrophenols was significantly enhanced with more negative cathode potential and shortened hydraulic retention time in the BESs. Moreover, the reduction of the three nitrophenols followed in the order of ONP > MNP > PNP in the BESs. Both quantum chemical calculation using density function theory and cyclic voltammetry analysis confirmed the reductive sequence of the three nitrophenols. In addition, the acute toxicity of nitrophenol effluent significantly decreased while its biodegradability was enhanced after treatment in the BES. Therefore, the BES technology offers bright prospects for efficient treatment of nitrophenol-containing wastewater. [Copyright &y& Elsevier]

Abstract: Biochemical oxygen demand (BOD) is a measure of biodegradable compounds in water and is, for example, a common parameter to design and assess the performance of wastewater treatment plants. The conventional method to measure BOD is time consuming (5 or 7 days) and requires trained personnel. Bioelectrochemical BOD sensors designed as microbial fuel cells (MFCs), which are systems where bacteria convert organic matter into an electrical current, have emerged as an alternative to the conventional technique. In this study, a new type of bioelectrochemical BOD sensor with features that overcome some of the limitations of current MFC-type designs was developed: (1) An external voltage was applied to overcome internal resistances and allow bacteria to generate current at their full capacity, and (2) the ion exchange membrane was omitted to avoid pH shifts that would otherwise limit the applicability of the sensor for wastewaters with low alkalinity. The sensor was calibrated with an aerated nutrient medium containing acetate as the BOD source. Linear correlation (R 2 = 0.97) with charge was obtained for BOD concentrations ranging from 32 to 1280 mg/L in a reaction time of 20 h. Lowering the reaction time to 5 h resulted in lowering the measurable BOD concentration range to 320 mg/L (R 2 = 0.99). Propionate, glucose, and ethanol could also be analyzed by the sensor that was acclimated to acetate. The study demonstrates a way to design more robust and simple bioelectrochemical BOD sensors that do not suffer from the usual limitations of MFCs (high internal resistance and pH shifts). [Copyright &y& Elsevier]

• G. sulfurreducens can synchronize current generation and biological nitrogen fixation. • Biological nitrogen fixation does not affect coulombic efficiency of MFC. • Anode respiration supports the biological nitrogen fixation of G. sulfurreducens. • Biological nitrogen fixation promotes the anode respiration of G. sulfurreducens. • Inoculation of G. sulfurreducens promotes N-deficient wastewater treatment. The present nitrogen fixation industry is usually energy-intensive and environmentally detrimental. Therefore, it is appealing to find alternatives. Here, we achieved both a synchronized biological nitrogen fixation and electric energy production by using Geobacter sulfurreducens in a microbial electrochemical system. The results showed that G. sulfurreducens was able to fix nitrogen depending on anode respiration, producing a maximum current density of 0.17 ± 0.015 mA cm−2 and a nitrogen-fixing activity of ca. 0.78 μmol C 2 H 4 mg protein−1 h−1, thereby achieving a net total nitrogen-fixing rate of ca. 5.6 mg L−1 day−1. Specifically, nitrogen fixation did not impair coulombic efficiency. Transcriptomic and metabolic analyses demonstrated that anode respiration provided sufficient energy to drive nitrogen fixation, and in turn nitrogen fixation promoted anode respiration of the cell by increasing acetate catabolism but reducing acetate anabolism. Furthermore, we showed that G. sulfurreducens could be supplied in a bioelectrochemical system for N-deficient wastewater treatment to relieve N-deficiency stress contributing to the formation of an electroactive biofilm, thereby simultaneously achieving nitrogen fixation, current generation and dissoluble organic carbon removal. Our study revealed a synergistic effect between biological nitrogen fixation and current generation by G. sulfurreducens , providing a green nitrogen fixation alternative through shifting the nitrogen fixation field from energy consumption to energy production and having implications for N-deficient wastewater treatment. [Display omitted] [ABSTRACT FROM AUTHOR]

• Biohythane production from MEC led to positive energy recovery • Dual-chamber MEC could stabilize biohythane generation and composition • Methanogen in single-chamber MEC consumed hydrogen to unbalance composition • CEM was key part to maintain hydrogen production for stable biohythane composition • Geobacter and methanogens were key species to affect biohythane composition Biohythane is alternative fuel to replace fossil fuel for car combustion, and biohythane generation could be potential pathway for energy recovery from wastewater treatment. Microbial electrolysis cell (MEC) is electrochemical technique to convert waste to methane and hydrogen gas for biohythane generation, but the feasibility and stability of MEC needs further investigation to assure sustainable energy recovery. System configuration is paramount factor for electrochemical reaction and mass transfer, and this study was to investigate the configuration impact (single vs dual chamber) of MEC for biohythane generation rate and stability. This study showed that dual-chamber MEC could separate methane and hydrogen gas production in the anode and cathode, and combined both together to produce biohythane. To reduce ohmic resistance for higher current, cation exchange membrane (CEM) was removed from dual-chamber to single-chamber MEC. However, free hydrogen diffusion was allowed in the single chamber since CEM was removed. The diffused hydrogen and substrate towards the cathode would favor the methanogen growth, and thus the hydrogen was consumed to reduce the biohythane generation and energy recovery efficiency (i.e., 7.5 × 10−3 reduced to 5.7 × 10−3 kWh kg−1 degraded COD day−1 after converting dual-chamber to single-chamber MEC). Absolute abundance of methanogen in single-chamber MEC was greatly boosted, as Methanosarcina and Methanobacteriale on the anode surface, increased by 132% and 243%, respectively, while the original dual-chamber MEC could maintain Geobacter growth for high current generation. This is the keystone study to demonstrate the importance of dual-chamber MEC for the feasibility and stability for the biohythane generation, building up the foundation to use electrochemical device to convert the organic waste to the alternative biohythane. [Display omitted] [ABSTRACT FROM AUTHOR]

• Sustainable technology for efficient treatment of nitrate and arsenite. • Bioelectrochemical NO3− reduction to N 2 with coulombic efficiency close to 100%. • Denitrifying biocathode formed by Sideroxydans sp. resistant to 5 mg As (III) L−1. • Arsenite oxidation catalytically and linked to denitrification by Achromobacter sp. The coexistence of different pollutants in groundwater is a common threat. Sustainable and resilient technologies are required for their treatment. The present study aims to evaluate microbial electrochemical technologies (METs) for treating groundwater contaminated with nitrate (NO 3 −) while containing arsenic (in form of arsenite (As(III)) as a co-contaminant. The treatment was based on the combination of nitrate reduction to dinitrogen gas and arsenite oxidation to arsenate (exhibiting less toxicity, solubility, and mobility), which can be removed more easily in further post-treatment. We operated a bioelectrochemical reactor at continuous-flow mode with synthetic contaminated groundwater (33 mg N-NO 3 − L−1 and 5 mg As(III) L−1) identifying the key operational conditions. Different hydraulic retention times (HRT) were evaluated, reaching a maximum nitrate reduction rate of 519 g N-NO 3 − m3 Net Cathodic Compartment d−1 at HRT of 2.3 h with a cathodic coulombic efficiency of around 100 %. Simultaneously, arsenic oxidation was complete at all HRT tested down to 1.6 h reaching an oxidation rate of up to 90 g As(III) m−3 Net Reactor Volume d -1. Electrochemical and microbiological characterization of single granules suggested that arsenite at 5 mg L−1 did not have an inhibitory effect on a denitrifying biocathode mainly represented by Sideroxydans sp. Although the coexistence of abiotic and biotic arsenic oxidation pathways was shown to be likely, microbial arsenite oxidation linked to denitrification by Achromobacter sp. was the most probable pathway. This research paves the ground towards a real application for treating groundwater with widespread pollutants. Image, graphical abstract [ABSTRACT FROM AUTHOR]

• High COD removal from flowback water producing low S2− in a potential-controlled BES. • Anode potential shaped a cryptical sulfur cycle with thiosulfate formation. • S 2 O 3 2− and SO 3 2− were preferentially biooxidized at −0.1 V Vs. SHE. • Anode with high potential likely shunted electrons and inhibited sulfite reduction. • Enriched bacteria and genes responsible for the novel sulfur cycle were identified. The flowback water (FW) from shale gas exploitation can be effectively treated by bioelectrochemical technology, but sulfide overproduction remains to be addressed. Herein, sulfate-reducing bacteria (SRB) meditated microbial fuel cells (MFCs) with anodic potential control were used. COD removal gradually increased to 67.4 ± 5.1% in electrode-potential-control (EPC) MFCs and 78.9 ± 2.4% in the MFC with open circuit (OC-MFC). However, in EPC MFCs sulfate removal stabilized at much lower levels (no more than 19.9 ± 1.9%) along with much lower sulfide concentrations, but in OC-MFC it increased and finally stabilized at 59.9 ± 0.1%. Partial sulfur reuse in EPC MFCs was indicated by the current production. Notably, thiosulfate was specially detected under low potentials and effectively oxidized in EPC MFCs, especially under -0.1 V vs. SHE, which probably related to the sulfur reuse. Metagenomics analysis showed that the anode with -0.1 and -0.2 V likely shunted electrons from cytochromes that used for reducing DsrC-S0 trisulfide and thus contributed to producing thiosulfate and decreasing sulfide production. Meanwhile, the anode with -0.1 V specially accumulated sulfur-oxidizing system (Sox) genes regarding thiosulfate and sulfite oxidation to sulfate, which concurred to the effective thiosulfate oxidation and also indicated the possible direct sulfite oxidation to sulfate during the sulfur cycling. But the anode of -0.2 V highly accumulated genes for thiosulfate and sulfite reduction. Both anodes also distinctly accumulated genes regarding thiosulfate oxidation to tetrathionate and sulfide oxidation to sulfur or polysulfide. Further, sulfur-oxidizing bacteria were specially enriched in EPC MFCs and likely contributed to thiosulfate and sulfite oxidation. Thus, we suggested that the higher electrode potential (e.g. -0.1 V) can shape a cryptic sulfur cycling, in which sulfate was first reduced to sulfite, and then reoxidized to sulfate by forming thiosulfate as an important intermediate or by direct sulfite oxidation. The results provide new sights on the bioelectrochemical treatment of wastewater containing complex organics and sulfate. Image, graphical abstract [ABSTRACT FROM AUTHOR]

The practical applications of membrane-based water treatment techniques are constrained by the problem of membrane fouling. Various studies have revealed that interactions between extracellular polymeric substances (EPS) and the membrane surface determine the extent of irreversible fouling. Herein, we describe a novel bioelectrochemical system (BES) integrated with an ultrafiltration (UF) membrane in order to provide an enhanced antifouling property. It was found that the integrated BES membrane system had a superior performance compared to a conventional (control) UF system, as manifested by a much lower development of transmembrane pressure. The BES significantly reduced microbial viability in the membrane tank and the imposed electrode potential contributed to the degradation of biopolymers, which favored the alleviation of membrane fouling. Notably, the electron transfer between the acclimated microorganisms and the conductive membrane in the BES integrated system exhibited an increasing trend with the operation time, indicating a gradual increase in microbial electrical activity. Correspondingly, the accumulation of extracellular polymeric substances (EPS) on the membrane surface of the BES integrated system showed a substantial decrease compared to the control system, which could be attributed to a series of synergistic effects induced by the BES integration. The differences in the microbial diversity between the control and the BES integrated system revealed the microbial selectivity of the poised potential. Specifically, microbial strains with relatively high EPS production, like the genus of Zoogloea and Methyloversatilis , were reduced significantly in the BES integrated system, while the expression of the electroactive bacteria was promoted, which facilitated extracellular electron transfer (EET) and therefore the bioelectrochemical reactions. Overall, this study has presented a feasible and promising new approach for membrane fouling mitigation during the process of water treatment. Image 1 • A BES integrated UF system was developed to alleviate membrane fouling. • e - transfer and microbial electrical activity can be enhanced by BES integration. • BES integration contributes to low EPS accumulation on membrane surface. • BES integration promotes the expression of specific electroactive bacteria. [ABSTRACT FROM AUTHOR]

Bioelectrochemical system (BES) is promising technology to simultaneously treat wastewater and recover energy, and electrode material is important for the system performance. Microbial fuel cell (MFC) is one of typical BES to be applied in wastewater treatment. How to improve the electrode material is significant to improve wastewater treatment, energy recovery and cost effectiveness. In this study, 3D-weaved carbon electrode entity, assembled by multiple pieces of carbon mesh (CM), was proposed to combine all electrode components as entity to facilitate electron conduction and ionic migration, compared with carbon brush (CB) and granular activated carbon (GAC). The result showed that current density and internal resistance of MFC using 3D-weaved CM as horizontally extended inside anode (CM(T)) were 30.9 A m-3 and 4.5 Ω, respectively, with higher output than traditional GAC (22.6 A m-3 and 6.2 Ω). Though GAC had greater electrode filling and surface area for biomass growth, the electron transfer efficiency per unit electrode biomass was only at 0.0019 ± 0.0002 mol g-1 d-1, much lower than CM(T) at 0.0077 ± 0.0009 mol g-1 day-1. Higher ionic migration rate of CM(T) suggested the assisting effect of composite electrode to enhance ionic transportation towards the cathode. Microbial analysis further indicated that 3D-CM electrode network could simultaneously enhance Geobacter abundance and methanogen activity, suggesting the importance of electrode network on electricigens. Furthermore, CM(T) could obtain 10 times higher energy output efficiency than traditional GAC when applied inside anode chamber. This study proved that network construction of anode electrode could promote the electrode performance and cost effectiveness, suggesting the future development of reactor design of bioelectrochemical system. Image 1 • Anode configuration impacted biomass growth and electron conduction. • 3D carbon mesh constructed as electron network entity for conducting facility. • Electrode entity construction greatly benefited electron generation efficiency. • 3D carbon mesh stimulated microbial activity and Geobacter growth. • Massive cost optimization achieved by 3D carbon mesh. [ABSTRACT FROM AUTHOR]

Bioelectrochemical systems (BES) can provide an energy efficient way to recover nutrients from wastewaters. However, the electron donors available in wastewater are often not sufficient to recover the total amount of nutrients. This work investigates hydrogen (H 2) as an additional substrate for bioanodes. This hydrogen can be produced in the fermentation of complex organic waste or could be recycled from the cathode. Understanding how to influence the competition of electroactive microorganisms (EAM) with methanogens for H 2 gas from different sources is key to successful application of H 2 as additional electron donor in bioelectrochemical nutrient recovery. Ethanol (EtOH) was used as model compound for complex wastewaters since it is fermented into both acetate and H 2. EtOH was efficiently converted into electricity (e−) by a syntrophic biofilm. Total recovered charge from 1 mM EtOH was 20% higher than for the same amount of acetate. This means that H 2 from EtOH fermentation was converted by EAM into electricity. Low EtOH concentrations (1 mM) led to higher conversion efficiencies into electricity than higher concentrations (5 and 10 mM). Thermodynamic calculations show this correlates with a higher energy gain for electrogens compared to methanogens at low H 2 concentrations. Cumulatively adding 1 mM EtOH without medium exchange (14 times in 14 days) resulted in stable conversion of H 2 to e− (67%–77% e−) rather than methane. With H 2 gas as electron donor, 68 ± 2% H 2 was converted into e− with no carbon source added, and still 53 ± 5% to e− when 50 mM bicarbonate was provided. These results show that under the provided conditions, electrogens can outcompete methanogens for H 2 as additional electron donor in MECs for nutrient recovery. Image 1 • Competition of electroactive microorganisms with methanogens over H 2 conversion was studied. • H 2 from EtOH was efficiently converted to electricity at low substrate concentrations. • EtOH grown biofilms showed competitive equality with methanogens for H 2 gas conversion. [ABSTRACT FROM AUTHOR]