Search

Microbial electrosynthesis (MES) has been proposed as a sustainable platform to simultaneously achieve wastewater treatment, renewable energy generation and chemicals production. Currently, the CO2 valorization via MES is restricted by the low production rate, while that via electrochemical reduction is limited by the production of C1 products with high efficiency and selectivity. The electrocatalyst-assisted MES could potentially solve these bottlenecks of both MES and electrochemical reduction technology by increasing the production rate and expanding the product range. Here, four types of metals were evaluated for mixed culture-based, electrocatalyst-assisted MES with the fabrication of electrical-biological hybrid cathodes. Cathodes based on In, Zn, Ti and Cu showed high parallelism at 30 A/m2. However, no parallelism was observed at 50 A/m2, and only Zn experienced a further increase of the maximum acetic acid production rate (1.23 ± 0.02 g/L/d, 313 ± 5 g/m2/d) and titer (9.2 ± 0.1 g/L), with the highest value of the production rate normalized to the project area of the fiber cathodes. Other volatile fatty acids and ethanol were below 0.5 g/L. Moreover, it was the sharp H2 generation, which mainly caused the fluctuation of coulombic efficiency. The application of such Zn-based electrical-biological hybrid system shall provide a more efficient route for CO2 valorization.

Carbon-rich waste materials (solid, liquid, or gaseous) are largely considered to be a burden on society due to the large capital and energy costs for their treatment and disposal. However, solid and liquid organic wastes have inherent energy and value, and similar as waste CO2 gas they can be reused to produce value-added chemicals and materials. There has been a paradigm shift towards developing a closed loop, biorefinery approach for the valorization of these wastes into value-added products, and such an approach enables a more carbon-efficient and circular economy. This review quantitatively analyzes the state-of-the-art of the emerging microbial electrochemical technology (MET) platform and provides critical perspectives on research advancement and technology development. The review offers side-by-side comparison between microbial electrosynthesis (MES) and electro-fermentation (EF) processes in terms of principles, key performance metrics, data analysis, and microorganisms. The study also summarizes all the processes and products that have been developed using MES and EF to date for organic waste and CO2 valorization. It finally identifies the technological and economic potentials and challenges on future system development.

Anaerobic granular sludge (AGS) is a promising technology for organic wastewater treatment and energy recovery. In this study, three different kinds of Fe and Fe oxides nanoparticles (Fe

To investigate the role of granular activated carbon (GAC) on nitrogen removal performance of elemental sulfur-based constructed wetlands (S0-based CWs), three systems were constructed according to the different configurations in the functional layer, namely S-CW (S0 added in the functional layer), CSC-CW (GAC, S0 and GAC placed in layers in the functional layer) and SC-CW (S0 and GAC mixed evenly in the functional layer). In CSC-CW and SC-CW, the volumetric ratio of S0:GAC was 9:1. Three CWs were operated under four different hydraulic retention times (HRTs) ranged from 48 h to 6 h. Over the experiment, total inorganic nitrogen (TIN) removal rates of the three CWs were 3.1 – 23.6 g m−2 d−1, 3.5 – 24.1 g m−2 d−1 and 3.4 – 11.5 g m−2 d−1, respectively; CSC-CW remained high TIN removal efficiency (from 74.7 ± 20.2 % to 93.4 ± 1.9 %) while SC-CW had significant lower values when HRT = 6 h (29.8 ± 30.1 %). Mass balance and high-throughput sequencing analysis revealed that mixotrophic denitrification at the sulfur layer and simultaneous nitrification-denitrification (SND) at the rhizosphere played the major role in N removal from CSC-CW (> 95 %). GAC addition facilitated the growth of Iris pseudacorus with the final fresh weight increased from 33.9 gFW ind−1 to 82.3 gFW ind−1 in CSC-CW and 82.7 gFW ind−1 in SC-CW. This study optimizes the practical application of S0-based CWs amended with GAC for N removal from carbon-limited wastewater.

This study constructed a 1000 L modularized MFC system, the largest volume so far, to treat practical municipal wastewater. This MFC system was operated under two different water flow connections in two municipal wastewater treatment plants (MWTP) for more than one year to test their treating abilities for wastewater with both low (average 80 mg L−1) and high initial COD concentration (average 250 mg L−1). The COD concentration in the effluent from the MFC system remained below 50 mg L−1 with a removal rate of 70–90%, which stably met the level A of the first class in discharge standard of pollutants for MWTP of China. A maximum power density of 125 W m−3 (7.58 W m−2) was generated when the MFC system was fed with artificial wastewater, while it lay in a range of 7–60 W m−3 (0.42–3.64 W m−2) when treating municipal wastewater. The energy recovery of 0.033 ± 0.005 kWh per m3 of municipal wastewater was achieved, with a hydraulic retention time (HRT) of 2 h.

Bioelectrochemical system (BES) is promising technology to simultaneously treat wastewater and recover energy, and electrode material is important for the system performance. Microbial fuel cell (MFC) is one of typical BES to be applied in wastewater treatment. How to improve the electrode material is significant to improve wastewater treatment, energy recovery and cost effectiveness. In this study, 3D-weaved carbon electrode entity, assembled by multiple pieces of carbon mesh (CM), was proposed to combine all electrode components as entity to facilitate electron conduction and ionic migration, compared with carbon brush (CB) and granular activated carbon (GAC). The result showed that current density and internal resistance of MFC using 3D-weaved CM as horizontally extended inside anode (CM(T)) were 30.9 A m-3 and 4.5 Ω, respectively, with higher output than traditional GAC (22.6 A m-3 and 6.2 Ω). Though GAC had greater electrode filling and surface area for biomass growth, the electron transfer efficiency per unit electrode biomass was only at 0.0019 ± 0.0002 mol g-1 d-1, much lower than CM(T) at 0.0077 ± 0.0009 mol g-1 day-1. Higher ionic migration rate of CM(T) suggested the assisting effect of composite electrode to enhance ionic transportation towards the cathode. Microbial analysis further indicated that 3D-CM electrode network could simultaneously enhance Geobacter abundance and methanogen activity, suggesting the importance of electrode network on electricigens. Furthermore, CM(T) could obtain 10 times higher energy output efficiency than traditional GAC when applied inside anode chamber. This study proved that network construction of anode electrode could promote the electrode performance and cost effectiveness, suggesting the future development of reactor design of bioelectrochemical system.

The application of in situ electrochemical generation of ferrous (Fe(II)) ions for phosphorus (P) removal in wastewater treatment was investigated with attention to the efficacy, kinetics and mechanism. At concentrations typical of municipal wastewater, P could be removed by in situ Fe(II) with removal efficiency higher than achieved on addition of FeSO4 and close to that of FeCl3 under both anoxic and oxic conditions. The generation of alkalinity due to water electrolysis at the cathode created much higher pH conditions compared to FeSO4 dosing thereby resulting in very different pathways of Fe solid phase formation and associated P removal mechanisms. The remarkably similar dependence of P removal on accumulated Fe for all investigated currents, initial P concentrations and DO conditions indicated that kinetic aspects did not play a role in P removal during in situ Fe(II) dosing. Thermodynamic modelling was undertaken to investigate possible solid phase formation pathways under anoxic conditions and these insights were extended to oxic conditions. The exclusive formation of ferrous hydroxide during anoxic in situ Fe(II) dosing implied that P removal occurred via coprecipitation and adsorption. Under oxic conditions, the high pH conditions would have resulted in rapid Fe(II) oxidation and formation of ferric oxyhydroxides with associated coprecipitation and adsorption effecting P removal in a similar pattern to that observed under anoxic conditions. In situ Fe(II) dosing represents a versatile option for chemical P removal with the precise control of Fe dosage to optimize FeP forms for possible P recovery.

Biohythane is alternative fuel to replace fossil fuel for car combustion, and biohythane generation could be potential pathway for energy recovery from wastewater treatment. Microbial electrolysis cell (MEC) is electrochemical technique to convert waste to methane and hydrogen gas for biohythane generation, but the feasibility and stability of MEC needs further investigation to assure sustainable energy recovery. System configuration is paramount factor for electrochemical reaction and mass transfer, and this study was to investigate the configuration impact (single vs dual chamber) of MEC for biohythane generation rate and stability. This study showed that dual-chamber MEC could separate methane and hydrogen gas production in the anode and cathode, and combined both together to produce biohythane. To reduce ohmic resistance for higher current, cation exchange membrane (CEM) was removed from dual-chamber to single-chamber MEC. However, free hydrogen diffusion was allowed in the single chamber since CEM was removed. The diffused hydrogen and substrate towards the cathode would favor the methanogen growth, and thus the hydrogen was consumed to reduce the biohythane generation and energy recovery efficiency (i.e., 7.5 × 10−3 reduced to 5.7 × 10−3 kWh kg−1 degraded COD day−1 after converting dual-chamber to single-chamber MEC). Absolute abundance of methanogen in single-chamber MEC was greatly boosted, as Methanosarcina and Methanobacteriale on the anode surface, increased by 132% and 243%, respectively, while the original dual-chamber MEC could maintain Geobacter growth for high current generation. This is the keystone study to demonstrate the importance of dual-chamber MEC for the feasibility and stability for the biohythane generation, building up the foundation to use electrochemical device to convert the organic waste to the alternative biohythane.

Flow-electrode capacitive deionization (FCDI), as a novel electro-driven desalination technology, has attracted growing exploration towards brackish water treatment, hypersaline water treatment, and selective resource recovery in recent years. As a flow-electrode-based electrochemical technology, FCDI has similarities with several other electrochemical technologies such as electrochemical flow capacitors and semi-solid fuel cells, whose performance are closely coupled with the characteristics of the flow-electrodes. In this review, we sort out the potentially parallel mechanisms of electrosorption and electrodialysis in the FCDI desalination process, and make clear the importance of the flowable capacitive electrodes. We then adopt an equivalent circuit model to distinguish the resistances to ion transport and electron transport within the electrodes, and clarify the importance of electronic conductivity on the system performance based on a series of electrochemical tests. Furthermore, we discuss the effects of electrode selection and flow circulation patterns on system performance (energy consumption, salt removal rate), review the current treatment targets and system performance, and then provide an outlook on the research directions in the field to support further applications of FCDI.

A major challenge for devising an electrochemically active biofilm (EAB)-based biosensor for real-time water quality early-warning is the formation of EAB that requires several days to weeks. Besides the onerous and time-consuming preparation process, the naturally formed EABs are intensively concerned as they can hardly deliver repeatable electrical signals even at identical experimental conditions. To address these concerns, this study employed sodium alginate as immobilization agent to encapsulate Shewanella oneidensis MR-1 and prepared EAB for devising a biosensor in a short period of less than 1 h. The artificial EAB were found capable of delivering highly consistent electrical signals with each other when fed with the same samples. Morphology and bioelectrochemical properties of the artificial EAB were investigated to provide interpretations for these findings. Different concentrations of bacteria and alginate in forming the EAB were investigated for their effects on the biosensor's sensitivity. Results suggested that lower concentration of bacteria would be beneficial until it increased to 0.06 (OD660). Concentration of sodium alginate affected the sensitivity as well and 1% was found an optimum amount to serve in the formation of EAB. A long-term operation of the biosensor with artificial EAB for 110 h was performed. Clear warning signals for incoming toxicants were observed over random signal fluctuations. All results suggested that the artificial EAB electrode would support a rapid devised and highly sensitivity biosensor.

A novel stacked microbial fuel cell (MFC) which had a total volume of 72 L with granular activated carbon (GAC) packed bed electrodes was constructed and verified to present remarkable power generation and COD removal performance due to its advantageous design of stack and electrode configuration. During the fed-batch operation period, a power density of 50.9 ± 1.7 W/m(3) and a COD removal efficiency of 97% were achieved within 48 h. Because of the differences among MFC modules in the stack, reversal current occurred in parallel circuit connection with high external resistances (>100 Ω). This reversal current consequently reduced the electrochemical performance of some MFC modules and led to a lower power density in parallel circuit connection than that in independent circuit connection. While increasing the influent COD concentrations from 200 to 800 mg/L at hydraulic retention time of 1.25 h in continuous operation mode, the power density of stacked MFC increased from 25.6 ± 2.5 to 42.1 ± 1.2 W/m(3) and the COD removal rates increased from 1.3 to 5.2 kg COD/(m(3) d). This study demonstrated that this novel MFC stack configuration coupling with GAC packed bed electrode could be a feasible strategy to effectively scale up MFC systems.

A new design of membrane capacitive deionization (MCDI) cell was constructed by packing the cell's flow chamber with granular activated carbon (GAC). The GAC packed-MCDI (GAC-MCDI) delivered higher (1.2-2.5 times) desalination rates than the regular MCDI at all test NaCl concentrations (∼ 100-1000 mg/L). The greatest performance enhancement by packed GAC was observed when treating saline water with an initial NaCl concentration of 100 mg/L. Several different GAC materials were tested and they all exhibited similar enhancement effects. Comparatively, packing the MCDI's flow chamber with glass beads (GB; non-conductive) and graphite granules (GG; conductive but with lower specific surface area than GAC) resulted in inferior desalination performance. Electrochemical impedance spectroscopy (EIS) analysis showed that the GAC-MCDI had considerably smaller internal resistance than the regular MCDI (∼ 19.2 ± 1.2 Ω versus ∼ 1222 ± 15 Ω at 100 mg/L NaCl). The packed GAC also decreased the ionic resistance across the flow chamber (∼ 1.49 ± 0.05 Ω versus ∼ 1130 ± 12 Ω at 100 mg/L NaCl). The electric double layer (EDL) formed on the GAC surface was considered to store salt ions during electrosorption, and facilitate the ion transport in the flow chamber because of the higher ion conductivity in the EDLs than in the bulk solution, thereby enhancing the MCDI's desalination rate.

The knowledge about membrane biofouling evolution in full-scale membrane bioreactor (MBR) applications is quite lacking, notwithstanding a few lab-scale investigations. For the first time, this study elaborated the effect of online NaOCl cleaning on the dynamic development of membrane biofilm microbiota during long-term operation of a large-scale MBR for municipal wastewater treatment (40,000 m3/d). Four times of membrane autopsies were conducted during 160 days operation to scrutinize the microbial community and concomitant organic foulants. The transmembrane pressure difference (TMP) development revealed limited effect of 30 min online NaOCl cleaning on long-term biofouling removal. NaOCl not only altered the structure of biofilm communities but also increased the richness and evenness on early fouling stages. Meanwhile, network analysis revealed the keystone taxa f_Comamonadaceae that played key roles in stabilizing community structure and developing anti-cleaning and irreversible fouling propensity of the biofilm. NaOCl cleaning also impacted the evolving of keystone taxa by intensifying the competition between the dominated taxa f_Moraxellaceae and other species during early fouling stages. Furthermore, the succession of the biofilm microbiota synchronously accelerated the TMP increase and the accumulation of organic foulants including polysaccharides, aromatic proteins and soluble microbial products during biofilm maturation. These identified key stubborn foulants shed light on limitations of current online NaOCl cleaning and provide guidance to optimize the efficiency of online chemical cleaning protocols in full-scale MBR operations.

This study employed a titanium mesh-membrane assembly (MMA) as the current collector in flow-electrode capacitive deionization (FCDI) device (designated as M-FCDI), and obtained a much reduced charge transport distance as compared to traditional FCDI with plate-shaped current collectors located far from the exchange membrane. The average salt removal rate of M-FCDI was greatly improved by 76% under 10 wt% carbon content than the control experiment with graphite plate as current collector, and the charge efficiency remained over 75% even under low carbon loading. This improvement was attributed to the reduced resistance as revealed by electrochemical impedance spectroscopy tests. Further investigation on FCDI's performance with different specifications of titanium meshes showed that the implementation of MMA could provide a larger effective electron transfer area, which would lead to better desalting performance.

Reducing the energy consumption of membrane bioreactors (MBRs) is highly important for their wider application in wastewater treatment engineering. Of particular significance is reducing aeration in aerobic tanks to reduce the overall energy consumption. This study proposed an in situ ammonia-N-based feedback control strategy for aeration in aerobic tanks; this was tested via model simulation and through a large-scale (50,000 m(3)/d) engineering application. A full-scale MBR model was developed based on the activated sludge model (ASM) and was calibrated to the actual MBR. The aeration control strategy took the form of a two-step cascaded proportion-integration (PI) feedback algorithm. Algorithmic parameters were optimized via model simulation. The strategy achieved real-time adjustment of aeration amounts based on feedback from effluent quality (i.e., ammonia-N). The effectiveness of the strategy was evaluated through both the model platform and the full-scale engineering application. In the former, the aeration flow rate was reduced by 15-20%. In the engineering application, the aeration flow rate was reduced by 20%, and overall specific energy consumption correspondingly reduced by 4% to 0.45 kWh/m(3)-effluent, using the present practice of regulating the angle of guide vanes of fixed-frequency blowers. Potential energy savings are expected to be higher for MBRs with variable-frequency blowers. This study indicated that the ammonia-N-based aeration control strategy holds promise for application in full-scale MBRs.

Large-scale membrane bioreactors (MBRs) have been widely used for the municipal wastewater treatment, whose performance relies on microbial communities of activated sludge. Nevertheless, microbial functional structures in MBRs remain little understood. To gain insight into functional genes and their steering environmental factors, we adopted GeoChip, a high-throughput microarray-based tool, to examine microbial genes in four large-scale, in-operation MBRs located in Beijing, China. The results revealed substantial microbial gene heterogeneity (43.7-85.1% overlaps) among different MBRs. Mantel tests indicated that microbial nutrient cycling genes were significantly (P 0.05) correlated to influent COD, [Formula: see text] -N, TP or sulfate, which signified the importance of microbial mediation of wastewater constituent removal. In addition, functional genes shared by all four MBRs contained a large number of genes involved in antibiotics resistance, metal resistance and organic remediation, suggesting that they were required for degradation or resistance to toxic compounds in wastewater. The linkages between microbial functional structures and environmental variables were also unveiled by the finding of hydraulic retention time, influent COD, [Formula: see text] -N, mixed liquid temperature and humic substances as major factors shaping microbial communities. Together, the results presented demonstrate the utility of GeoChip-based microarray approach in examining microbial communities of wastewater treatment plants and provide insights into the forces driving important processes of element cycling.