1. Biofilm formation by a biotechnologically important tropical marine yeast isolate, Yarrowia lipolytica NCIM 3589.
- Author
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Dusane DH, Nancharaiah YV, Venugopalan VP, Kumar AR, and Zinjarde SS
- Subjects
- Biofilms drug effects, Carbon pharmacology, Hydrogen-Ion Concentration drug effects, Hydrophobic and Hydrophilic Interactions, Plankton drug effects, Salinity, Yarrowia cytology, Yarrowia growth & development, Yarrowia physiology, Biofilms growth & development, Biotechnology, Seawater microbiology, Tropical Climate, Yarrowia isolation & purification
- Abstract
Biofilm formation by Yarrowia lipolytica, a biotechnologically important fungus in microtitre plates, on glass slide surfaces and in flow cell was investigated. In microtitre plates, there was a short lag phase of adhesion followed by a period of rapid biofilm growth. The fungus formed extensive biofilms on glass slides, whereas in flow-cells a multicellular, three-dimensional microcolony structure was observed. The isolate formed biofilms in seawater and in fresh water media at neutral pH when grown in microtitre plates. The carbon sources differentially affected formation of biofilms in microtitre plates. Lactic acid, erythritol, glycerol, glucose and edible oils supported the formation of biofilms, while alkanes resulted in sub-optimal biofilm development. A variation in the morphology of the fungus was observed with different carbon sources. The results point to the possible existence of highly structured biofilms in varied ecological niches from where the yeast is isolated., (IWA Publishing 2008.)
- Published
- 2008
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