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In order to study the influence of natural mordant on dyeing performance of gallnut wool fabric, plant dyestuffs of gallnut was extracted by decoction method, and cold mineral water of ferrosiliceous bicarbonate hydrochloric acid was used as natural mordant. The single factor experiments of related influencing factors were carried out, and the changing rules of influencing factors on color characteristic were analyzed and summarized. The results showed that the dyeing process, the mass fraction of natural mordant, the pH value of dye solution and the number of dyeing times had certain effects on the color characteristic value, among which the dyeing process > the mass fraction of natural mordant > the number of times of dyeing > the pH value of dye solution. The optimum process of gallnut wool fabric co-mordant dyeing with natural mordant is as follows: bath ratio 1 :30, dyeing time 30 min, mordant dyeing time 15 min, dyeing temperature 80 °C, dyeing liquid mass fraction 100% ( pH value 6 during pre-heating), natural mordant mass fraction 100%. By increasing the number of dyeing, the color depth can be increased, and the optimal number of dyeing is 5 times. [ABSTRACT FROM AUTHOR]