1. Phytase produced on citric byproducts: purification and characterization.
- Author
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Spier, M. R., Fendrich, R. C., Almeida, P. C., Noseda, M., Greiner, R., Konietzny, U., Woiciechowski, A. L., Soccol, V. T., and Soccol, C. R.
- Subjects
PHYTASES ,CITRUS ,PELLETIZING ,CHROMATOGRAPHIC analysis ,PHYSIOLOGICAL effects of temperature ,WASTE products ,ASPERGILLUS niger - Abstract
Citric pulp is an agro-industrial residue from the citrus processing industry with low inorganic phosphorus content applied in animal feed. A new bioprocess was developed to produce and purify a new phytase generated on citric pulp fermentation by Aspergillus niger FS3. The phytase was purified by cationic-exchange, anionic-exchange chromatography and chromatofocusing steps. From SDS-PAGE analysis, the molecular weight of the purified phytase was calculated to be 108 kDa. The phytase had an optimum pH of 5.0-5.5 and an optimum temperature of 60°C. The phytase displayed high affinity for phytate, and the K was 0.52 mM. The purified phytase was sufficiently able to withstand pelleting temperatures, retaining sufficiently high phytate-degrading activity. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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