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This paper deals with the thermal comfort assessment in two business offices in the same building, located in Belgrade, Serbia. They are approximately the same size, on the same floor, but on the opposite sides of the building. Office 1 is South-West orientated and Office 2 is North-East oriented, with one occupant in each office. One occupant is male and the other one is female, so the gender difference in thermal sensation votes (TSV) was taken into the consideration. During the researched period, the following thermal comfort parameters were measured: air temperature, relative humidity, radiant temperature, and air velocity. The input value for the occupants’ activity was 1.2 met, which corresponds to the sedentary office activity. The clothing level was adopted for the summer period, which corresponds to the value of 0.6 clo. The measurements were performed two times per day, during one working week. The assessment was performed for the spring period, when heating and cooling devices were turned off. The results of measurements showed that South West orientated office had higher air temperatures, approximately 0.5 to 1.5°C, in comparison with Office 2 which was North East orientated. The female occupant was satisfied with higher air temperatures in the office, voting as neutral, while the male occupant had the impression of a neutral environment for a set of parameters with a lower air temperature.