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Objective: To study the chemical constituents of Clerodendron philippinum var. simplex., Methods: The ethanol extract of Clerodendron philippinum var. simplex was isolated and purified by extraction, chromatography on silica gel and recrystallization. The structures of compounds isolated were identified by physicochemical properties and spectral analysis., Results: Four compounds were isolated and identified as friedelin (1), 25 (27)-dehydroporiferasterol (2), 22-dehydroclerosteryl acetate (3), uncinatone (4)., Conclusion: Compound 2, 3 are isolated form this genus for the first time, Compounds 4 is isolated from this plant for the first time.

Objective: To investigate the chemical constituents of the roots of Berchemia lineate as a medicinal plant of Yao nationality in China., Methods: Compounds were isolated by various column chromatography and elucidated by physicochemical and spectral analysis., Results: Nine compounds were isolated from the 95% ethanol extract of the roots of Berchemia lineate and their structures were identified as palmitic acid (1), octadecanoic acid (2), beta-sitosterol (3), stigmasterol (4), fernenol (5), chrysophanol (6), physcion (7), floribundiquinone D (8), 2-acetylphyscion(9) respectively., Conclusion: Compounds 1-4,7-9 are isolated from this plant for the first time,and compounds land 2 are firstly isolated from this genus.

Objective: To study the chemical constituents of Yao Medicine Cissus pteroclada., Methods: The compounds were isolated and purified by column chromatography with silica gel, TLC and recrystallization. Their structures were elucidated on the basis of physicochemical properties and spectra analysis., Results: Six compounds were isolated and identified as beta-sitosterol (I), bergenin (II), 11-O-galloylbergenin (III), 11-O-(4-hydroxy benzoyl) bergenin (IV), gallic acid (V), daucosterol (VI)., Conclusion: Compounds III and NIV are obtained from the genus for the first time. All the compounds are isolated from this plant for the first time except the compound II.

Objective: To study the chemical constituents of Phyllodium elegans., Methods: The compounds were isolated and purified by extraction, chromatography on silica gel and recrystallization. Their structures were elucidated on the basis of physicochemical properties and spectra analysis., Results: Three triterpenoids were isolated and identified as lupenone (1), lupeol (2), betulin (3)., Conclusion: Compound 2 is obtained from the genus for the first time, Compounds 1-3 are isolated from this plant for the first time.

Objective: To study the chemical constituents of Blumea laciniata., Methods: The chemical constituents of the ethyl acetate fraction of ethanol extract from Blumea laciniata were isolated with column chromatographic techniques. The structures of the isolated compounds were elucidated by spectroscopic analysis and comparison with their published datas., Results: Five compounds were isolated and identified as: protocatechuic acid (1), chrysoeriol (2), apigenin (3), 4-hydroxy-3,5-dimethoxbenzoic acid (4), scopolet (5)., Conclusion: Compounds 1 - 5 are isolated from this plant for the first time, and also obtained from this genus for the first time.

Objective: To study the chemical constituents of Pithecellobium clypearia., Methods: The compounds were isolated from Pithecellobium clypearia with column chromatography. Their structures were characterized on the basis of their physical and chemical preperties, as well as chromatographic and spectroscopic evidences., Results: A pair of isomeric flavans consisting of 7, 3'-O-di-gallyoltricetiflavan (I) and 7,4'-O-di-gallyoltricetiflavan (II), along with a catechin compound (-)-epigallocatechin-7-O-gallate (III) were isolated from the leaves and twigs of Pithecellobium clypearia., Conclusion: Compound I is a new flavan. Compound III is isolated from Pithecellobium genus for the first time, and its 13C-NMR data is firstly given in the present study.

The chemical constituents of Adina pilulifera, which were traditionlly used in the area of Yao minority in Southern China, were isolated and characterized. Two compounds were obtained from the ethyl acetate fraction of ethanol extract of Adina pilulifera, and one compound was obtained from the n-butanol fraction of ethanol extract of Adina pilulifera. Their structure were characterized by spectroscopic analysis and comparison with published data to be sarracenin (1), 2-methyl-5, 7-dihydroxychromone (2) and morroniside (3). Except compound 2, other two compounds were isolated from this plant for the first time, and they were also obtained from the Adina genus for the first time.

Objective: To detect the cytotoxicity and in vitro antiproliferative effects of the flavones compounds isolated from some Yao herbal medicines, and analyze the basic structure-activity relationship., Methods: The cytotoxicity on normal cells and antiproliferative effect on tumor cells were tested by MTT reduction assay respectively., Results: Among the 6 flavones, the Eriodictyol-7-O-a-D-glucoside, Naringenin-7-O-beta-D-glucoside, quercetin and quercetin-3-O-beta-D-glucoside showed very low cytotoxicity to the two normal cells, Vero and MC cells, while the naringenin was high toxic to both of them. The eriodictyol was no toxic to Vero Cell but could affect MC cell at low concentration. The naringenin exhibited a wide-spectrum antiproliferative effect on all of the 7 tested cancer cell lines especially on the MCF-7 with low IC50 about 50 microg/ml. Among these cancer cells, the MCF-7 and HepG2 were more sensitive to the flavones compounds than the others. Both of them were inhibited by eriodictyol, quercetin and naringenin even at low test concentration. Furthermore, the antiproliferative effects of these compounds were concentration-related., Conclusion: Some of flavones compounds isolated from some Yao herb medicines showed high antiproliferative effect on cancer cells with low cytotoxicity on normal cells.

The chemical constituents of Ardisia chinensis, which traditionally used in the area of Yao minority in Southern China, were analyzed and characterized. Seven compounds were obtained from the ethyl acetate fraction of ethanol extract of Ardisia chinensis. These 7 compounds were characterized by spectroscopic analysis and comparison with published data to be salicylic acid (1), 4-hydroxy-3-methoxy-benzoic acid (2), gallic acid ethyl ester (3), 4-hydroxy-3,5-dimethoxy benzoic acid methyl ester (4), protocatechuic acid (5), gallic acid (6) and catechin (7). All of them were isolated from this plant for the first time, and compound 7 was obtained from this genus for the first time.