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Objective: To study the protective effect of Saussurea involucrata alcohol extract on liver mitochondria in mice under hypoxia condition., Methods: The hypoxia mice model was established, the BALB/c mice were randomly divided into four groups:normal group ,hypoxia model group, positive control group and Saussurea involucrata alcohol extract group. Mice were put into low pressure oxygen chamber and decompressed, adapted to hypobaric hypoxia environment of simulated altitude of 8,000 m for 12 h, and then recovered to normal altitude. The mice were sacrificed and the liver mitochondria was isolated, the mitochondrial membrane potential and the activity of malate dehydrogenase, aconitase, α-ketoglutarate dehydrogenase, pyruvate dehydrogenase and mitochondrial complex I, II and V were measured., Results: Compared with hypoxia model group, Saussurea involucrata alcohol extract protected mitochondrial membrane potential, sustained the activities of aconitase, α-ketoglutarate dehydrogenase, pyruvate dehydrogenase, and mitochondrial complex I, II and V under hypoxia condition., Conclusion: Saussurea involucrata alcohol extract can protect the liver mitochondrial function in mice under hypoxia condtion.

Objective: To investigate the chemical constituents with anti-hypoxia activity from Saussurea involucrata., Methods: The chemical constituents, isolated and purified by column chromatography from Saussurea involucrata, were identified by several spectroscopic methods. The anti-hypoxic activities of these compounds were examined using the normobaric hypoxic model of mice., Results: Twelve compounds were isolated from petroleum ether extract of Saussurea involucrata and identified as n-octacosane (1), 1-undecanol (2), heptadecan-l-ol(3), heptacosan-1-ol(4), myristicin (5), apiol(6), β-sitosterol(7), lupeol(8), moslosooflavone (9), mosloflavone (10), negletein(11), and 5, 6-dihydroxy-7, 8-dimethoxyflavone(12)., Conclusion: All compounds except 7 and 8 are isolated from this plant for the first time. Compound 1, 5 and 8 - 12 can significantly prolong the survival time of hypoxic mice.

Objective: To estimate the effect of ethanol extract from Saussurea involucrata (EES) on biochemical indicators of simulated high-altitude hypoxia induced mice and its mechanism., Methods: The oxidative stress indicator( MDA content, SOD activity) and metabolism parameters (LD content, LDH activity, ATP content, Na+ -K+ -ATPase and Ca2+ -Mg2+ -ATPase activity) in both brain and heart of the simulated high-altitude hypoxia induced mice were detected., Results: Compared with the model group, the ESS group could significantly increase the activity of SOD and LDH and decrease the content of MDA and LD in both brain and heart, the content of ATP and the activity of Na+ -K -ATPase and Ca2+ -Mg2+ -ATPase were also elevated., Conclusion: The results demonstrate that EES can increase the antioxidant ability, decrease the injury of free radical and ease the disfunction of energy metabolism caused by hypoxia.

Objective: To study the chemical constituents of Abelmoschus esculentus., Methods: The chemical constituents were isolated and purified by chromatography on silica gel and recrystallization. The chemical structures were elucidated on the basis of physicochemical properties and spectral date., Results: Twelve compounds were isolated and identified as 9,19-23 (Z)-cycloart-23-en-3beta, 25-diol(1), ergost-7,22-diene-3beta,5alpha,6beta-triol(2),5alpha, 6alpha-epoxyergost-8(14), 22-dien-3beta, 7alpha-diol(3), 5alpha,8alpha-epidioxyergost-22-en-3beta-ol(4), stigmast-5-ene-3beta,7alpha-diol(5), stigmast-5, 22-diene-3beta,7alpha-diol(6), stigmast-4-en-3beta,6beta-diol(7), stigmast-4,22-diene-3beta,6beta-diol(8), stigmast-4-ene-3, 6-dione(9), stigmast-4-ene-3-one (10),beta-sitosterol(11), daucosterol(12)., Conclusion: Compounds 1-10 are obtained from the plant for the first time and also from the genu for the first time.