1. [Subcellular localization of severe fever with thrombocytopenia syndrome virus in macrophages].
- Author
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Han Y, Li YX, Jin C, Li C, Qu J, Zhang FS, Zhang QF, Liang MF, and Qiu PH
- Subjects
- Cell Line, Tumor, Endoplasmic Reticulum virology, Golgi Apparatus virology, Humans, Bunyaviridae isolation & purification, Fever virology, Macrophages virology, Thrombocytopenia virology
- Abstract
Objective: To study the subcellular localization of severe fever with thrombocytopenia syndrome virus (SFTSV) in macrophages and understand the replication and assembly mechanism of SFTSV in host cells., Methods: Using two types of human macrophage cell lines THP-1 and U937, the study analyzed the intracellular colocalization of SFTSV with Golgi apparatus and endoplasmic reticulum by immunefluorescence staining and confocal microscopy., Results: SFTSV infected macrophage cell lines THP-1 and U937. Immunofluorescence staining showed that the SFTSV nuclear protein colocalized with Golgi apparatus and closely surrounded by endoplasmic reticulum in the perinuclear region., Conclusion: The results suggested that Golgi complex and endoplasmic reticulum are probably the sites for formation and maturation of SFTSV viral particles.
- Published
- 2013