1. [Expression of cyclooxygenase-2, hMLH1 and hMSH2 proteins, and their relationship with microsatellite instability in gastric carcinoma].
- Author
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Song WQ, Han CL, Chen Y, Zhang YH, Wei JY, and Liu Y
- Subjects
- Adaptor Proteins, Signal Transducing genetics, Cyclooxygenase 2 genetics, Humans, MutL Protein Homolog 1, MutL Proteins, Neoplasm Proteins genetics, Nuclear Proteins genetics, Stomach Neoplasms metabolism, Adaptor Proteins, Signal Transducing biosynthesis, Cyclooxygenase 2 biosynthesis, Microsatellite Repeats genetics, Neoplasm Proteins biosynthesis, Nuclear Proteins biosynthesis, Stomach Neoplasms genetics
- Abstract
Objective: To investigate the expression of cyclooxygenase-2 (COX-2), human mut-l homologue 1 (hMLH1) and human mut-s homologue 2 (hMSH2) proteins in human paired gastric carcinoma (GC) and adjacent normal mucosa, and analyze their relationship with microsatellite instability (MSI)., Methods: The protein expressions were examined by western blotting. Five MSI loci were assessed by PCR., Results: In 30 surgically excised GC tissues, the overexpression rate of COX-2, the low expression rate of hMLH1 and hMSH2 were 66.7%, 40% and 33.3%, respectively. Significant differences were found when compared with those of adjacent normal mucosa (P < 0.05). MSI was detected in 13 GC. The number of MSI-H (MSI-High, > or = 2 loci), MSI-L (MSI-Low, only one locus), and MSS (microsatellite stable) were 9, 4 and 17, respectively. The number of low expression rates of COX-2, hMLH1 and hMSH2 in MSI-H were 6, 8 and 5, respectively. There were significant differences compared to that of MSS (P < 0.05)., Conclusion: The results suggest that microsatellite instability pathway is probably involved in the carcinogenesis of gastric carcinoma, which is frequently accompanied by low expression of hMLH1 and hMSH2, and may be also by low expression of COX-2.
- Published
- 2005