Search

Experiments were carried out 17 V. foetus strains, out of which 13 had been isolated from cattle, 2 from swine and 2 from sheep. Among them, on the basis of biochemical, morphological and serological studies, was selected one strain, by the aid of which was produced afterwards. Both the antigen and the antiserum showed high qualities as to activity and specificity. The study included the influence of peptone in a nourishing medium, pH of the medium, as well as the quantity of CO2 during the cultivation and conservation of the strain, and on the morphology, activity and specificity of the diagnostic antigen produced out of it. It was established that the most suitable pH of the medium for cultivating the strain used for the production of antigen in diagnosing vibriosis, as well as the production medium itself was pH = 7.0. A substitution of atmospheric air by 15% CO2 during the cultivation of V. foetus strain for production, alongside the other conditions, ensures a high yield in the production of diagnostic antigen and a high serological activity. For the conservation of the production strain in is necessary to keep it at a constant temperature of +22 degrees c. This condition contributes to the conservation of biochemical, morphological and serological qualities and ensures a high yield of the antigen obtained, used in vibriosis.

Obtained were type-specific fluorescent vibrionic antisera for the laboratory identification of Vibrio fetus strains. They produced positive fluorescence only with the homologous strains, and did not react with the heterologous strains. This showed that the sera were strongly specific. Their use is suggested for the differentiation typing of the Vibrio strains.